Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F84 I1 R1	23	34.8	1446234	89.3%	1291486	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	74.1 / NA	27	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/16); total (11/16)

TTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGA  >  NC_000913/754307‑754574

ttCGCGGGAGGCGGTTGAGGTGAGGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGtt                                                                                                                               <  2:74689/143‑1 (MQ=255)

       gATGCGGCTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCttt                                                                                                                        <  1:553484/143‑1 (MQ=255)

            ggTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCACCTCGGAGGCTGGGTTTTACTTTTGTaa                                                                                                                   >  2:623017/1‑142 (MQ=255)

                     gaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAttt                                                                                                          <  1:242955/143‑1 (MQ=255)

                       gcgtggggTCAAAGGTGCATGGACCTGTTGAACCGAGAGTACGGATATCAATAACATCTTGACCGAGGGGGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                        <  1:717313/138‑1 (MQ=255)

                       agCCTGGGTCACAGGTGACCGCACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGGACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                        <  1:97215/143‑1 (MQ=255)

                                cAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                               >  2:398707/1‑143 (MQ=255)

                                 aaaGTTGAACCGACCTTTTGAACCGTGAGTACGGATATCAATAACATCTTGTCCCAGCGTGCCTTTCAGCACATCCTGTTCCACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCCCCATTTAAGGTTTCCTTa                                                                                              <  2:592276/143‑1 (MQ=255)

                                             acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTTTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                  <  1:464735/143‑1 (MQ=255)

                                             acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGc                                                                                  <  1:143659/143‑1 (MQ=255)

                                               cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGATCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTTTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                <  1:176445/143‑1 (MQ=255)

                                               cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCTTTGAGGGTGGGATTTGCTTTTGTAACAGCCATTTAAGGTCTCCTTAGCTCCTTATTGCGt                                                                                >  1:289050/1‑143 (MQ=255)

                                               cTTTTGAACCGAGAGTACGGATATCAATAACATCCTGACACAGCGTGCCTTTCAGCACATCCAGTTCAACGGCTGTATCCCCGTTGCGAGCGGGTTTTGCTTTTCTCTCAGCCATTTAAGCTCGCCTTAGCGCCTTATTACgc                                                                                >  2:29433/1‑142 (MQ=255)

                                                               aCGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACtt                                                                >  1:616380/1‑143 (MQ=255)

                                                                 ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCTATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                              <  1:656081/143‑1 (MQ=255)

                                                                   atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGGTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTCGCGGAACTTAGAt                                                            >  2:556376/1‑143 (MQ=255)

                                                                      tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAtgtg                                                         >  2:366588/1‑143 (MQ=255)

                                                                       cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCATAGCGCCTTATTTCGTAAGACTGCCGGAACTTAAATTTGc                                                        >  1:23541/1‑143 (MQ=255)

                                                                                cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcaca                                               >  2:558722/1‑143 (MQ=255)

                                                                                              ccTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTa                                 >  2:277667/1‑143 (MQ=255)

                                                                                               cTTTCAGCACCGCCCGTTCAACAGCTGTATCCCCGTTGAGGCTCGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                <  2:59483/143‑1 (MQ=255)

                                                                                                               ttCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGc                >  1:155929/1‑143 (MQ=255)

                                                                                                                   aCAGCTGTCGCCCCGTTGAGGGTTGGTTTTGCTCTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCTGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc            <  2:392228/143‑1 (MQ=255)

                                                                                                                    cAGCTGTATCGCCGTTGCGGGTGGGGTTTGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgcc           <  1:574622/143‑1 (MQ=255)

                                                                                                                           atacgcGTTGGGGGCGGGTTTTGTTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt    <  1:711255/138‑1 (MQ=255)

                                                                                                                            tCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgtg   <  1:398707/143‑1 (MQ=255)

                                                                                                                             ccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGa  <  1:558722/143‑1 (MQ=255)

TTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGA  >  NC_000913/754307‑754574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A