Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F66 I1 R1	21	40.5	1611144	86.0%	1385583	142.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	94.7%	80.5 / ‑4.8	38	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/16); minor base T (0/2); total (20/18)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

CGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754309‑754584

cGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGTACATCCAGTTAAACAGCTGTATACCAGTTGAGGGTGGGttat                                                                                                                                       >  2:39984/1‑141 (MQ=255)

      aTGCGGTTGAAGTGAAGCCTGTGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                 <  1:13070/143‑1 (MQ=255)

            ttGAAGTGAAGCCTTGGTCGAAGGTGAACACACCTTTTGAACCGAGAGTCCGGATATCTATAACAGCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                           <  2:88615/143‑1 (MQ=255)

            ttGAAGTGAAGCCTGTGTCAAAGGTGATCACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                           <  1:383503/143‑1 (MQ=255)

             ttaagtgaagCCTGGGGCAGAGGTGAACACAGCTGTTGGACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAg                                                                                                                          <  2:30631/141‑1 (MQ=255)

             tgaagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAg                                                                                                                          >  1:409200/1‑143 (MQ=255)

                  tgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTATCAGCACATCCAGCTCAAGAACTGTATCGCCGCTACTGCTGGGTCTTGCTCTTGTATCatcgctc                                                                                                                     >  2:719319/1‑137 (MQ=255)

                      gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACCTCCAGTTCAGCAGCTGTATTCCCAATGAAAGTGGATATAAAATTTGTATCAGACATTTAAg                                                                                                                 >  1:15104/1‑143 (MQ=255)

                                         acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                              <  1:743764/143‑1 (MQ=255)

                                         acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCATTAACGACTGAtt                                                                                              >  1:320729/1‑143 (MQ=255)

                                         acacCTTTTGAACCGAGAGGACGGATATCAATAACATCTTGACCCAGTGTGCTTTTTAGCACATCTAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                              <  1:340048/143‑1 (MQ=255)

                                            ccTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGTGGGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCg                                                                                           <  2:552540/143‑1 (MQ=255)

                                                     ccGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTGGTATCAGCCATTTCAGGTCTCCTTCGCGCCTTATTGCGTAAAACTga                                                                                  >  1:31716/1‑142 (MQ=255)

                                                      tgagaGTACGGATATCAATATCATGTTGACCCAGCGTGCATTTCAGCACATCGAGTTCAACAGCTGTAGCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGcc                                                                                 <  1:765351/142‑1 (MQ=255)

                                                          agTACGGATATTAATATCATCTTGACCCAGCGTGCCTGTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                             <  1:4267/143‑1 (MQ=255)

                                                               ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGATCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                        >  1:60556/1‑143 (MQ=255)

                                                               ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCATATATATAAGACATTTAAGGTCTCATTAGAGCCTTATTGCGTAAGAGTACCGGAACTTaa                                                                        >  1:600597/1‑143 (MQ=255)

                                                                  tatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGATCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAtt                                                                     >  2:535725/1‑143 (MQ=255)

                                                                   atCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGGCTCCTTAGCGCCTTATTGACTAATACTGCCGGAACTCAAAtca                                                                    >  2:370546/1‑141 (MQ=255)

                                                                      aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGcc                                                                 >  2:89382/1‑143 (MQ=255)

                                                                          aCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg                                                             <  1:71389/143‑1 (MQ=255)

                                                                           cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGCACTTAAATTTGCCTTCGc                                                            >  1:528365/1‑143 (MQ=255)

                                                                                   cccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCTGAACTTAAATTTGCCTTCGCACaacaac                                                    >  2:364659/1‑143 (MQ=255)

                                                                                       gCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTgg                                                <  2:527536/143‑1 (MQ=255)

                                                                                            gcTTGCAGCAGATCCAGTTCAGCAGCTGTATCCTCGTTGATGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTa                                           <  2:771424/142‑1 (MQ=255)

                                                                                             ctctgtgCACATCCAGTTCATAAGCTGTCTCCACGTTCACGTTGTGTCTTGCTTTTGTATCAGCCATTTAAAGTATTCTTCCCGCCTTAGTGCGTAAGACTGCCGGAACTTATATTTGCCTTCGCACATCAACCTGGCTTTAc                                          <  2:254427/137‑1 (MQ=255)

                                                                                             cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCAATTTACGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCAGGAACTTAAATTTGCCTTCCCACATCACCCTGGCatttc                                          >  2:265566/1‑141 (MQ=255)

                                                                                                      caTCCAGTTCAATAGCTGTATCCCCGTTTAGGCTGTTTTTTGCTTTTGTATCATCTATTTAAGTTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                                 <  1:754057/143‑1 (MQ=255)

                                                                                                      caTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                                 <  1:305207/143‑1 (MQ=255)

                                                                                                       aTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTGGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTa                                >  2:785950/1‑143 (MQ=255)

                                                                                                        tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCGCATCAACCTGGCTTTACCCGTTTTTTAt                               >  1:79296/1‑143 (MQ=255)

                                                                                                                 aCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgc                      <  1:762100/143‑1 (MQ=255)

                                                                                                                 aCAGCTGTATCCCCGTTGAACGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGACTTATTGCATAAGACTGCCGGAACTTAAATTTGCCTTCGCACACCAACCCAGCTCTACCCCTTTGTGATTTTGcgcgt                      >  2:122463/1‑142 (MQ=255)

                                                                                                                   aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTATCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTGTACCCGAATTTTATTTGTCTctccg                    >  2:183681/1‑143 (MQ=255)

                                                                                                                     cTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGGCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGGACTTAAATTTGCCTTCGCACATCACCCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGct                  <  2:87088/143‑1 (MQ=255)

                                                                                                                      tGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGctc                 <  1:107427/143‑1 (MQ=255)

                                                                                                                                    gggtgggtTTTGCTTTTTTATCAGTCATTTAAGGTCTCCTTAGCGCCTTAAAGCGGAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGa   <  1:98100/143‑1 (MQ=255)

                                                                                                                                     ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCCCATCCACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  >  1:787344/1‑143 (MQ=255)

                                                                                                                                     ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGAACATAGCCCGTGCGTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  >  1:579204/1‑143 (MQ=255)

CGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754309‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A