Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F119 I0 R1	76	56.4	2152892	90.0%	1937602	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	150.1 / NA	55	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/28); total (27/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754310‑754584

gCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTg                                                                                                                                      >  1:543933/1‑143 (MQ=255)

 cAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGc                                                                                                                                     <  1:894408/143‑1 (MQ=255)

  aGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCt                                                                                                                                    <  2:56234/143‑1 (MQ=255)

  aGGATGCGGTTGAAGCGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGGTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCt                                                                                                                                    <  2:907711/143‑1 (MQ=255)

   ggATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTTGGTTTTGcat                                                                                                                                   >  1:501570/1‑141 (MQ=255)

         gtgtcaagtgcagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGACATCCATAACATCGTGACCCAGCGCGCCTTTCAGCACATCCAGTTCAACAGCTGGATCCCCGTTGAGGGTGGGTTTTGCTTTTGTAt                                                                                                                             <  2:816867/138‑1 (MQ=255)

                gtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTCTGTATCAGCCAt                                                                                                                      >  1:279228/1‑143 (MQ=255)

                    agccgtggTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGAGATCAATCACATCTTGACCCAGCGTGCCTTTCAGCACATCTCGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                                  <  2:734566/137‑1 (MQ=255)

                       cTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGt                                                                                                               >  1:974789/1‑143 (MQ=255)

                       cTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGt                                                                                                               >  1:45436/1‑143 (MQ=255)

                              aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                        >  1:516407/1‑143 (MQ=255)

                              aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTGCCCGCACATCCAGTTCAACAGCTGTATCCCCGTTGGGGGTGGGGTTCGCTGGCGCATCAGCACTTCAAGGTCTCCATa                                                                                                        >  2:867702/1‑143 (MQ=255)

                                aGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgc                                                                                                      >  1:636509/1‑143 (MQ=255)

                                        acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTATTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                              <  2:66273/143‑1 (MQ=255)

                                            cTTTTGAGCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGCGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                          <  2:515834/143‑1 (MQ=255)

                                             ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGGGGGTCGGTCTTGCTGTTTTATCAGCCACTTAAGGTCTCCTTCGCGCCTTATTGCGtc                                                                                         >  1:39730/1‑142 (MQ=255)

                                              ttttAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        <  1:508702/143‑1 (MQ=255)

                                                tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                      <  2:107404/143‑1 (MQ=255)

                                                  agccGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTACGGTCTCCTTAGCGCCTTATTGCGTAAGACt                                                                                    >  2:768916/3‑143 (MQ=255)

                                                   aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTg                                                                                   >  2:584600/1‑143 (MQ=255)

                                                   aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTCTGCTGTTGTATCACCCATTTCAGGTCTCCGTGTCGTCTTGTTGCCTAacaccg                                                                                   >  2:857800/1‑141 (MQ=255)

                                                     cGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCCTTGAGGGTGGGTTTCGCTTTTGTTTCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCCTACGACTGcc                                                                                 >  2:121203/1‑143 (MQ=255)

                                                       agagTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCgg                                                                               >  2:389524/1‑143 (MQ=255)

                                                                  attaaTAACAGCTTGTCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                    <  2:543933/140‑1 (MQ=255)

                                                                    cactAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCTCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                                  <  2:371124/140‑1 (MQ=255)

                                                                       tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                               >  1:973561/1‑143 (MQ=255)

                                                                        aaCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCttt                                                              >  1:115838/1‑142 (MQ=255)

                                                                          cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCTTTGAGGGTGGTTTTTTCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGc                                                            <  1:620486/143‑1 (MQ=255)

                                                                                gACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTACATTTGCCTTCGCACATcc                                                      >  2:205350/1‑142 (MQ=255)

                                                                                   ccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCCAATCAAcc                                                   >  2:100917/1‑143 (MQ=255)

                                                                                   ccAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAAcc                                                   >  1:758441/1‑143 (MQ=255)

                                                                                             tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAca                                         >  2:28846/1‑142 (MQ=255)

                                                                                              ttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAccc                                        >  1:573841/1‑143 (MQ=255)

                                                                                               tCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACCTAAATTTGCCTTCGCACATCAACCCGGCTTTACCCg                                       >  2:22082/1‑143 (MQ=255)

                                                                                                  gCACAGCCAGTTCAACAGCTGTATTCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                    <  1:932803/143‑1 (MQ=255)

                                                                                                    acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGACGGAACTTAAATTTGCCTCCGCACATAAACCTAGCTTTACCCGttttt                                  >  2:324774/1‑143 (MQ=255)

                                                                                                    acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTCAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttttt                                  >  1:881908/1‑143 (MQ=255)

                                                                                                     caTCCAGTTCAACAGCTGTATCCTCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttttt                                 <  2:616978/143‑1 (MQ=255)

                                                                                                      aTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTa                                <  1:584600/143‑1 (MQ=255)

                                                                                                           ggtCAACAGCTGTATCCCCGTGGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg                           <  2:420484/141‑1 (MQ=255)

                                                                                                               aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCCTTTTTTATTTGGCTcc                       >  2:649017/1‑142 (MQ=255)

                                                                                                               aaCAGCTGTACCCCCGTTGAGGGTGGGTTTTGCTCTAGTGTCAGCCGGGTAAGGTCTCCTTAGCGCCGTATTGCGTAAGACTGCCCGACCTTAATTTTGCCTTCGCACTTCATCCTGGCTTTACCCGTTTTTTATTTGGCTcg                       <  1:955897/143‑1 (MQ=255)

                                                                                                                  aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                    >  2:567072/1‑143 (MQ=255)

                                                                                                                      gTATCCCCGTGGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGctct                <  1:95519/143‑1 (MQ=255)

                                                                                                                       tATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGACATCGGAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtg               <  1:601874/143‑1 (MQ=255)

                                                                                                                        tgccccGTTGAGGGGGGTTTGTGTTGTTGGAGCAGCCATGTGAGGTCTCCTTAGCGCCTTAGTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  2:279228/141‑1 (MQ=255)

                                                                                                                        aTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  2:187116/143‑1 (MQ=255)

                                                                                                                        aTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  1:891925/143‑1 (MQ=255)

                                                                                                                            ccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCACACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa          >  1:294346/1‑143 (MQ=255)

                                                                                                                                tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTATATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  2:330696/143‑1 (MQ=255)

                                                                                                                                tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  2:45436/143‑1 (MQ=255)

                                                                                                                                tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg      <  1:542988/143‑1 (MQ=255)

                                                                                                                                tGAGGGTCGGTTTTGCTTTTGTGTCTGCCATTTAGGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAGCTTATATTTGCCTGCGCACATCAACCTGGCTTTCCACGTTTTTAATGTTTCTCGCCGCTCTGTGAAAGAgg      <  2:130755/143‑1 (MQ=255)

                                                                                                                                 gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg     <  1:795487/143‑1 (MQ=255)

                                                                                                                                    ggTGGGTTGTGCTTTTGTATCAGCCGTTTAAGGTCTCCGTAGCGCCCTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACAGCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  <  2:637444/143‑1 (MQ=255)

GCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754310‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A