Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F124 I0 R1	44	29.6	1159012	90.6%	1050064	142.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	111.7 / NA	38	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/18); total (20/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAA  >  NC_000913/754311‑754585

cAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTCCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTCGAGGGTGGGCTTTGc                                                                                                                                       >  1:242429/1‑143 (MQ=255)

 aagctGCGGTTGAAGCGAAGTCTGGGGCACAGGCGAACACACCCCACCCACCACTATCACGACTACCAAAAGCACCCTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCt                                                                                                                                      <  2:562598/139‑1 (MQ=255)

     ttcGGTTGAAGTCAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTg                                                                                                                                  <  1:537949/141‑1 (MQ=255)

       cGGTTTACGTGAAGCCTGAGTCAATGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTAGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTa                                                                                                                                <  1:58498/143‑1 (MQ=255)

         gTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTCACGGCGGGTTTCGCTTTTGTagc                                                                                                                              >  2:486061/1‑141 (MQ=255)

          ttGATGTGAAGTCTGTGTCAAAGATGAACACACCTTTTGAAACGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCa                                                                                                                             <  1:513112/143‑1 (MQ=255)

             aagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGATGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGcc                                                                                                                          >  1:184578/1‑143 (MQ=255)

                   agCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGCAAGGGTAAAAATAACAGCTTGACCAAGATTGCCTTTCAGTATATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                                    <  1:243290/143‑1 (MQ=255)

                    gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                   <  1:170842/143‑1 (MQ=255)

                       tGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCATGCCTTTCAGCACATCCAGTTCAACAGCTGTATACCCGTTGAGGGCGGGTTTTGCTTTCGCATCAGCCGTTTAAGGtc                                                                                                                >  2:65326/1‑143 (MQ=255)

                                   gAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCt                                                                                                    <  1:462474/143‑1 (MQ=255)

                                       acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                                <  2:139333/143‑1 (MQ=255)

                                         acCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCCTATTGc                                                                                              >  1:542853/1‑143 (MQ=255)

                                          ccTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTGTGCTTTTGTATCAGCCATTTAAGGTCTCCCTCGCGCCTTATTgag                                                                                             >  2:18911/1‑141 (MQ=255)

                                           cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                            >  1:267463/1‑143 (MQ=255)

                                               tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                        <  2:267463/143‑1 (MQ=255)

                                                   ccGAGAGTACGGATATCACTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                                    <  1:486061/143‑1 (MQ=255)

                                                    cGAGAGAACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATACCCGTTGAGGGGGGGTTTTGCTTTTGTAGCAGCCAATTCAGGTCTCGTTAGCGCCTTGTTGAGTAAGACTGc‑c                                                                                  >  2:294752/1‑143 (MQ=255)

                                                              gATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGGGTAAGACTGC‑CGGAGCTTaaa                                                                        >  1:154153/1‑143 (MQ=255)

                                                                      tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCtt                                                                <  1:386154/143‑1 (MQ=255)

                                                                          aTCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAGGACTGC‑CGGAACTTAACTATGCCTACgac                                                            >  2:116803/1‑141 (MQ=255)

                                                                            cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGcaca                                                          <  1:459204/143‑1 (MQ=255)

                                                                              tGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATc                                                        <  1:221049/143‑1 (MQ=255)

                                                                                           cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGCATGCACTGCA‑TAAAATTTGCCTTCGCACATCCACCTGGCTTCAc                                           >  2:406663/1‑143 (MQ=255)

                                                                                            tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTGAATTTGCCTTCGCACATCAACCTGGCTTTAcg                                          >  1:23700/1‑142 (MQ=255)

                                                                                             ttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAccc                                         >  1:466356/1‑143 (MQ=255)

                                                                                                  cacaTCCAGTTCGACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttt                                    <  2:360896/143‑1 (MQ=255)

                                                                                                      tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGCAACTTAAATTTGCCTTTGCACATCAACCTGGCCTTACCCGTTTTTTAt                                >  1:207600/1‑143 (MQ=255)

                                                                                                         aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTg                             >  2:452826/1‑143 (MQ=255)

                                                                                                                  gCTGTGTCCCCCGTGAGGGTTGGTTTTGACGTTTTTTGAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CTGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccgc                    <  2:5389/143‑1 (MQ=255)

                                                                                                                   cTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGct                   >  2:470681/1‑143 (MQ=255)

                                                                                                                       ttCCCCGTTGAGGGTGGGTTTTGCTTGTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCGACCTGGCTTTTCCTGTGTTTTATTTGGCTCGCCGCTCtgt               <  2:206548/142‑1 (MQ=255)

                                                                                                                            cGTTGAGGGTGGGTTTTGCTTTTGTATAAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑AGGAACTTAAATTTGCCTTCGCACGTCAACCTGGCTTTCCACGTTTTATATTTTGCTAGCCGCTCTGTGAAag          >  1:172468/1‑143 (MQ=255)

                                                                                                                             gTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga         <  2:516013/143‑1 (MQ=255)

                                                                                                                             gTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATGTAAGGTCTCCTTAGCGCCTTAATGAGTAAAACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga         <  1:214811/143‑1 (MQ=255)

                                                                                                                                gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg      >  1:344516/1‑143 (MQ=255)

                                                                                                                                gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg      >  1:344948/1‑143 (MQ=255)

                                                                                                                                    gTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATCTTGCTCGCCGCTCTTTGCAAGAGGGGcaa  >  2:206582/1‑143 (MQ=255)

CAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGC‑CGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAA  >  NC_000913/754311‑754585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A