Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F21 I1 R1	19	60.0	2284426	90.7%	2071974	142.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	97.9%	135.4 / NA	47	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (19/27); total (20/27)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAA  >  NC_000913/754315‑754585

aggCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                  <  2:1037182/141‑1 (MQ=255)

    ggTCGAAGTCAAGCCTGGGTCAAAGGTCAACACACCTTTTGAACCGAGAGTACGGATATCAATCACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTGTTGCTTTTGTAt                                                                                                                              <  1:19693/143‑1 (MQ=255)

        taagtgaagCCTGGGTCAAAGGTGAACATACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGc                                                                                                                          <  1:867416/142‑1 (MQ=255)

                gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                  >  2:705255/1‑143 (MQ=255)

                gCCTGGGTCAAAGGTGAACACACCTGTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                                  <  1:1010940/143‑1 (MQ=255)

                 ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGGTGAGGGTGAGTGTTGCTTTTGTATCAGCCATTTAAgg                                                                                                                 >  1:1024449/1‑143 (MQ=255)

                  cTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCATTTCAGCACATCCAGCTCACCCGCTGTATCCCCGTTGCTGGCAGCTCTGGGTTTTGTATCCGCCATTTTAGGt                                                                                                                >  2:332039/1‑143 (MQ=255)

                      gTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTcc                                                                                                            >  1:625495/1‑143 (MQ=255)

                       tCACAGGTGAACACACCTTTTCAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCt                                                                                                           <  2:477541/143‑1 (MQ=255)

                             gTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTTTATCCCCGTTGAGGGTTGGTTTTGCTTTTGTATCAGCCCTTTAGAGTCTCCTTAgcgc                                                                                                     >  2:607255/1‑143 (MQ=255)

                                aaCACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCtt                                                                                                  <  1:740494/143‑1 (MQ=255)

                                     acCTTTTGAACCGAGAGTACGGATATCAATAATATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCGCCTTTTAAGGTCTCCTTGGAGCTTTATTgg                                                                                             >  2:354482/1‑142 (MQ=255)

                                       cTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCATTTAACATTGAGTTGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGt                                                                                           <  1:524296/143‑1 (MQ=255)

                                        ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTa                                                                                          <  2:777137/143‑1 (MQ=255)

                                                    aggaCGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                              <  1:792644/140‑1 (MQ=255)

                                                          gATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaaa                                                                        <  2:203384/143‑1 (MQ=255)

                                                             atCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                     <  1:58228/143‑1 (MQ=255)

                                                                aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTACATTTGcc                                                                  >  2:543795/1‑143 (MQ=255)

                                                                 aTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTACGTTTGCCt                                                                 >  1:182014/1‑143 (MQ=255)

                                                                  tAACCTCTTGACCCAGCGTGCCTTTCAGCATATCCAGTTCAATAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                                <  2:945304/143‑1 (MQ=255)

                                                                    gcgccTTGGCCCAGCGTGCCTTTCGGCACCTCCCGTTCAACAGCGGTAGCCCCGTTGAGGGTGGGTTTTGCTTTTGTAGCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg                                                              <  1:449393/139‑1 (MQ=255)

                                                                            ccccGGCGTGCCTTTCAGCACATCTAGTTCAGCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                                                      <  2:945502/142‑1 (MQ=255)

                                                                                  cGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTCTTGCTTTTGTATCAGCCATTTAAGTTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGc                                                <  1:1019704/143‑1 (MQ=255)

                                                                                      ccTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTa                                            >  2:430073/1‑143 (MQ=255)

                                                                                      ccTTTAAGCACATCCCGTTCCTCAGCTGTCTTCTCGTTGAGTGTGGTTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTACGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTa                                            <  2:89769/143‑1 (MQ=255)

                                                                                         ttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCCTCGCACCTCCACCTGGCTTCAccc                                         >  2:452969/1‑143 (MQ=255)

                                                                                            aGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTCCTTTTGTATCAGCCATTTTAGGTCTCCTCAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACATGATTTTAACAGtt                                      >  1:61228/1‑143 (MQ=255)

                                                                                             gCACATCCAGTTCAACAGCTGTAGCCCCGTTGAGGGTGGGTTTTGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCGTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                     <  1:752216/143‑1 (MQ=255)

                                                                                              cacaTCCAGTTCAACAGCTGTATCCCCGTAGAGGGTGGGGTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtttc                                    >  2:406265/1‑142 (MQ=255)

                                                                                                   ccAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCGCATCACCCGGGCTTTACCCCTTTCGTAtt                               >  2:941762/1‑143 (MQ=255)

                                                                                                    cAGTTCAACAGCTGTATCCCCGTTTAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                              <  1:354482/143‑1 (MQ=255)

                                                                                                    cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                              <  1:705255/143‑1 (MQ=255)

                                                                                                    cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                              <  1:430073/143‑1 (MQ=255)

                                                                                                    cAGTTCAACAGCCGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTCAGCGGCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                              <  1:581892/143‑1 (MQ=255)

                                                                                                             aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgccg                     >  1:249997/1‑143 (MQ=255)

                                                                                                               cggaaaccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGct                   >  1:165192/7‑143 (MQ=255)

                                                                                                               cTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGATTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTTGTTCGCCGct                   >  2:670443/1‑143 (MQ=255)

                                                                                                                    tCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGACTCTTGTGTTGCTCGCCGCTCtggg              >  2:276221/1‑141 (MQ=255)

                                                                                                                        cGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaacg          >  1:809377/1‑141 (MQ=255)

                                                                                                                         gTTGAGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga         <  1:104345/143‑1 (MQ=255)

                                                                                                                         gTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga         <  1:101592/143‑1 (MQ=255)

                                                                                                                          ttGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTCTACCCGTTTTTTATTTTGCTCGCTGCTCTGTGAAagac        >  2:976788/1‑142 (MQ=255)

                                                                                                                          gtGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGTTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTTGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAagag        <  1:1095245/142‑1 (MQ=255)

                                                                                                                            gAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCGTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg      <  1:137618/143‑1 (MQ=255)

                                                                                                                            gAGGGTGGGTTTTCCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCTTAGTGCGTAAGACTGCCGGGACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg      <  2:940850/143‑1 (MQ=255)

                                                                                                                             aGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGTCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgggg     <  1:712724/143‑1 (MQ=255)

                                                                                                                                gTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaaa  >  2:916831/1‑143 (MQ=255)

ATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAAA  >  NC_000913/754315‑754585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A