Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F18 I1 R1	19	60.0	2279412	90.4%	2060588	142.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	97.5%	111.6 / NA	40	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (27/12); minor base C (1/0); total (28/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754313‑754584

gcttgaggTTGAAGTGAATCCTGGGTCAAAGGTTAACTCTCCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACAGCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtt                                                                                                                                   <  1:242107/137‑1 (MQ=255)

           aagtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCGcc                                                                                                                        >  2:22430/1‑143 (MQ=255)

              tgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACGTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCAtt                                                                                                                     >  1:509397/1‑143 (MQ=255)

              tgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAtt                                                                                                                     >  1:596625/1‑143 (MQ=255)

                aagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCGTGCATCACACCCAGTTCAACAGCTGTCTCTCCGTAGAGGGTGGCTTTTGTATTTGTGTCACACATTTa                                                                                                                   >  2:456445/1‑143 (MQ=255)

                  gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCCACAGCTGTATCCCCGTGGAGGGAGGGTTTTGATCTTGTACCCGCCATTCAAg                                                                                                                 >  2:496840/1‑143 (MQ=255)

                  gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGCTGCGGGTGGGTCTTACTTTTGTATCCGCCATCTAAg                                                                                                                 >  2:496295/1‑143 (MQ=255)

                    cTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGGTTTGCTTTTGTATCAGCCATTTAAGGt                                                                                                               >  1:336710/1‑143 (MQ=255)

                       ggTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCCGCGTGCCATTCAGCACATCCAGTTCAACAGCTGTGTCCCCGCAGAGGGTGCGTTTTGCTTACGTATCAGCCATATAAggacac                                                                                                            >  2:369752/1‑139 (MQ=255)

                               gTGATCACAGCTTTTTAGCCTGGAGAACAGATAGCAATACCTTCATGACCCAGCGTGCCTGTCAGCACATCCAGGTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAgcgc                                                                                                    <  1:151335/143‑1 (MQ=255)

                                          ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGGGGCGGGTGGGTTTTGCTTTTGTGTCAGCCATTTAAGGTCTCCTTAGCGCCCTCTTggtta                                                                                         >  1:453519/1‑139 (MQ=255)

                                                 ccGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGc                                                                                  >  1:908913/1‑143 (MQ=255)

                                                  cGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTCTTGCGTAAGACTGcc                                                                                 >  2:1067705/1‑143 (MQ=255)

                                                      agTCCGGATATCAATAACATCCTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGaa                                                                             <  1:223615/143‑1 (MQ=255)

                                                       gTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCGCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAc                                                                            >  1:872658/1‑143 (MQ=255)

                                                       gTACGGAGATGAATAACATCTCGACCCAGCGTGCCTTTCAGGACATCTAGTTCATCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCGTCTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAc                                                                            <  2:450609/143‑1 (MQ=255)

                                                           ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAGCATaa                                                                        >  2:146560/1‑143 (MQ=255)

                                                           ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACCGCCGCACCTTaa                                                                        >  2:642835/1‑143 (MQ=255)

                                                           ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTGTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                        <  1:402750/143‑1 (MQ=255)

                                                            gagatCAATAACAGCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaaa                                                                       <  2:812141/140‑1 (MQ=255)

                                                            gATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaaa                                                                       <  2:510074/143‑1 (MQ=255)

                                                             atatCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAt                                                                      <  1:630221/143‑1 (MQ=255)

                                                                tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTCTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTCTTTCGTTACACTGCCGGATCTTCAAttct                                                                   >  2:972476/1‑141 (MQ=255)

                                                                  aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTATCTTTGcc                                                                 >  1:763459/1‑143 (MQ=255)

                                                                  aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTccc                                                                 >  2:738104/1‑143 (MQ=255)

                                                                   aTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCt                                                                >  1:997907/1‑143 (MQ=255)

                                                                     agtaTCTTGTGCCAACGTGCCTTTAAGCAAATCTAGTTCAACAGCTATATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                              <  1:490910/140‑1 (MQ=255)

                                                                       cATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGCCTGCCGGCACTTAAATTTGCCTTCGc                                                            >  2:647343/1‑143 (MQ=255)

                                                                         tCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcac                                                          >  2:831825/1‑143 (MQ=255)

                                                                           ttGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTCCCTTCGcacct                                                        >  2:446704/1‑141 (MQ=255)

                                                                                         gtttGAGCACATCCAGTTCAACTGCTGTGTCACCGTTGAGTGTGGGTTTTGTTTTTGTATTAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCTGGAACTTAAATTTGCCTTCGTACATCAACCTGGCTTTAc                                          <  2:224840/142‑1 (MQ=255)

                                                                                              aGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTCAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTCTGCCCGtt                                     >  1:619520/1‑143 (MQ=255)

                                                                                               gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                    >  1:151410/1‑143 (MQ=255)

                                                                                               gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                    >  1:151514/1‑143 (MQ=255)

                                                                                                 acaTCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCCttttt                                  >  2:423918/1‑143 (MQ=255)

                                                                                                    tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCCACCTGGCTGTACCCGTGTTTTag                               >  1:179883/1‑142 (MQ=255)

                                                                                                         ttCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCCCATCAACCTGGCTTTACCCGTTTTTTATTTGGc                          >  2:717504/1‑143 (MQ=255)

                                                                                                                      tCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTATACCCGTTTTTTATTTGGCTCGCCGCTCtgtg             <  2:594237/143‑1 (MQ=255)

                                                                                                                            ttGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCACGTCCCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAagag       <  1:369732/143‑1 (MQ=255)

                                                                                                                                 ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTACGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGATAGAGGGGaa  >  1:1119119/1‑143 (MQ=255)

GGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754313‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A