Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F59 I0 R1	60	41.1	1578944	90.5%	1428944	142.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	754,446	A→G	100%	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	100.0%	118.5 / NA	40	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/16); total (24/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754306‑754584

gttCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACTCCTTTTGAACCGAGAGTACGGACATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAgggtgggt                                                                                                                                          <  2:435842/142‑1 (MQ=255)

aTTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAgggtgggt                                                                                                                                          <  2:435839/143‑1 (MQ=255)

       ggATGCGGTTGAAGTGAAGCCTGGGGCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtt                                                                                                                                   <  2:299195/143‑1 (MQ=255)

         cggCGGGTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATACCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                 <  1:220260/141‑1 (MQ=255)

         aTGCGGTTGAAGTGGAGTCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACTTCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                                 <  2:268884/143‑1 (MQ=255)

                          ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTCTCAGCCATTTAAgg                                                                                                                >  2:572399/1‑143 (MQ=255)

                          ccTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCAGTTCAgg                                                                                                                >  1:717427/1‑143 (MQ=255)

                                 cAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                         >  1:383651/1‑143 (MQ=255)

                                 cAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                         <  2:621986/143‑1 (MQ=255)

                                  aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTGCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCGTTTCAGGTCTCCTTa                                                                                                        >  2:391577/1‑143 (MQ=255)

                                   aaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAg                                                                                                       >  1:675329/1‑143 (MQ=255)

                                     ggTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACCTCCAGTTCACGAGCTGTCTCCTCGTTGAGGGCGGGTCTTGCCTTTGTACCAGGCCTTTGAGGGAGCCTTAgtc                                                                                                     >  2:725625/1‑141 (MQ=255)

                                     ggTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATGCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCGTTTGTATCAGCCATTTCACGTCCACTTAtcg                                                                                                     >  2:562788/1‑143 (MQ=255)

                                       tGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGcc                                                                                                   <  1:95221/143‑1 (MQ=255)

                                            acgccTTTCGAACCGAGAGTACGCACGTCAACAACAGCCTGCCCCACCGTGCCTTTCAGCACATCCAGTTCGACACCTGTATCCCCGTTGAGGGTGGGTTTTGCTTCTGTATCAGCCATTTAAGGGCTCCTTAGCGCCTTAtt                                                                                              <  2:115646/140‑1 (MQ=255)

                                            acacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                              <  1:77089/143‑1 (MQ=255)

                                                  tttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTaa                                                                                        >  1:153824/1‑143 (MQ=255)

                                                          gagaGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCGGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCg                                                                                <  2:331085/143‑1 (MQ=255)

                                                                  ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGGGGGTTTTGCTTTTTTATCAGCCATTTAAGGTCTCCTTAGCGCCAAATTGCGTAAGACTGACGGAACTaaa                                                                        >  1:684745/1‑143 (MQ=255)

                                                                       tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTg                                                                   <  2:736435/143‑1 (MQ=255)

                                                                        cAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                                  <  1:762409/143‑1 (MQ=255)

                                                                         aaTAACATCTTGACCCAGCGTGCCTTTCAGCACCTCCAGTTCAACCGCTGTATCCCCGTTGAGGGCGGGTTTCGCTCTTGTATCAGCCGTTTAAGGTCTCCTTAGCGCCTTCTTGCGTACGACCGCCCGAACTCACATCTGcc                                                                 >  2:55336/1‑143 (MQ=255)

                                                                         aaTAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGAGTGCCGGAACTTAAATTTGcc                                                                 >  1:453678/1‑143 (MQ=255)

                                                                           tAACATCTCGCCCCAGCGTGTCTTTCACCACACTCAGTTCCACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGCATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                               <  2:422161/143‑1 (MQ=255)

                                                                            aaCAGCTTTACCCAGAGTGCCTTGCAGCACACCCAGCTCAACAGCTGTAGCCGCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                              <  1:481866/143‑1 (MQ=255)

                                                                                 cTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGGCGCCTTAGCGCCTTTTTGCGTAAGACTGCCGGAGATTAAGGTTTCCTTCGcgca                                                         >  1:544206/1‑143 (MQ=255)

                                                                                  ttGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGGGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACAt                                                        >  2:653575/1‑143 (MQ=255)

                                                                                  ttGACACAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGcacgt                                                        >  2:136141/1‑141 (MQ=255)

                                                                                     aCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCaa                                                     >  1:770820/1‑143 (MQ=255)

                                                                                             tGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCtt                                             >  1:373072/1‑143 (MQ=255)

                                                                                              gCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGccgt                                            >  1:262904/1‑140 (MQ=255)

                                                                                                   tCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCTTCGCACATCAACCCGGCTTTACCCg                                       >  2:516601/1‑143 (MQ=255)

                                                                                                          aTCCAGTTCAACAGGTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCGCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTa                                <  1:682568/143‑1 (MQ=255)

                                                                                                           tCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACCTCAACCTGCCTTTACCCGTTTTTTac                               >  2:373651/1‑142 (MQ=255)

                                                                                                            ccAGTTCAACAGCTGTATCCCCGTTGAGGGTGGATATTGATTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTCTTTAtt                              >  2:770071/1‑143 (MQ=255)

                                                                                                              aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCATTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACATGGCTTTACCCGTTTTTTATTTg                            >  1:28137/1‑143 (MQ=255)

                                                                                                                   aaCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcg                       <  1:747139/143‑1 (MQ=255)

                                                                                                                            gtCCCGGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAGCGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCATCCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt              <  2:764933/142‑1 (MQ=255)

                                                                                                                              ccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGa            >  1:174498/1‑143 (MQ=255)

                                                                                                                                     gAGGGTGGCTTCGGCTTTTGTCTCATCCATTTAAGCTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAggg     <  1:559510/143‑1 (MQ=255)

                                                                                                                                      aGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTCCCCGTTTTCTATTTGGCTCGCCGCTCTGTGAAAGAgggg    >  1:368642/1‑143 (MQ=255)

                                                                                                                                        ggTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGaa  >  1:732421/1‑143 (MQ=255)

ATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGGGGAA  >  NC_000913/754306‑754584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A