Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F2 I0 R1 193 6708.0 8620653 98.5% 8491343 136.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,020,229 T→G 12.9% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,020,2290TG12.9% 316.0 / 9.9 175V133G (GTC→GGC) yjbBNa/Pi cotransporter family protein
Reads supporting (aligned to +/- strand):  ref base T (47/99);  new base G (22/5);  total (71/104)
Fisher's exact test for biased strand distribution p-value = 2.31e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTG  >  NZ_CP022686/3020090‑3020366
                                                                                                                                            |                                                                                                                                         
gggggTGCAGATGTCGGGACGGCGATGATGGCGCGTATTCTCCCCTTCTATATATCCTGGCTGTGACCGTTACTTCTTTTTATCGGCGTGTTTTTTTTCCTAGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  7:976043/138‑1 (MQ=255)
cTGGGTGCATATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCATCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  1:824976/140‑1 (MQ=255)
cTGGGTGCAGATGTCGTGCCGGCGCTGATGGCGAGTATTCTCACCTTCGATTTATCCTGGCTGTCACAGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCTGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  4:1026828/140‑1 (MQ=255)
cTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  8:45226/140‑1 (MQ=255)
cTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  1:187333/140‑1 (MQ=255)
cTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  5:236205/140‑1 (MQ=255)
cTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  8:378934/140‑1 (MQ=255)
cTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  2:987394/140‑1 (MQ=255)
cTGGGTGCAGATGGCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  2:705654/140‑1 (MQ=255)
cTGGGTGAAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTGCGATTTATCCTGGCGGTCACGGTTACTTATTTTTATCGGCGTGATTTTTTACCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGt                                                                                                                                           <  1:714419/140‑1 (MQ=255)
 tGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCAACGTTTCTTATTTTTATCTGCGTCTTTTTTTTCCTCAGACGGACAAAGTCACGCCCCCGGCAACTGGG‑CCGCGTc                                                                                                                                          >  8:298633/1‑140 (MQ=255)
  gggTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACGAGGTCGCCAAAAGTCAACCGCCGGGGCACAGGG‑CCgcggcg                                                                                                                                         >  1:559240/1‑140 (MQ=255)
      gCAGATGTCGGGACGGCGCTGATGGCGCGTATTATCACCTTCGATTTATCCTTGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCTGTAt                                                                                                                                     <  4:790849/140‑1 (MQ=255)
        aGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTGCTCGGGCGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTAGGTAttt                                                                                                                                   >  3:450817/1‑139 (MQ=255)
        aGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTG‑GATTTTTACTCGGGCGCAAACAGTCACCCGCCGGGCACCTGGG‑CCGCGTGGGTAtttg                                                                                                                                  >  7:212073/1‑138 (MQ=255)
             tCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCCAACAGTCACGCGCCGGGCCACTGGG‑CCGCGGGGGTATTGGTGtt                                                                                                                              >  2:666102/1‑140 (MQ=255)
             tCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACACGGGCGCAAACAGTCCCGCGCCGGGCAACTGGG‑GCGCGGCGGGATTTGTCtt                                                                                                                              >  1:1055823/1‑140 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTGCACGGCCGCAAACAGTCACCCGCCGGGCAACAGGG‑GCGCGTCGGGTTTGGTCTTgg                                                                                                                            >  7:163411/1‑140 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACCGTCACGCGCCGGGCAACTGGG‑CCGGGTCGGTATTGGTCTTgg                                                                                                                            >  2:761631/1‑140 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGAATTTTTTGCTCCGTCGCCAACACACACGCCCCGGGCCACAGGG‑CCGCGGGGGTATTGGTCGTgg                                                                                                                            >  5:948722/1‑140 (MQ=255)
                ggACGGCGCTGATGGCGCGTGTTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTggg                                                                                                                           <  4:132920/140‑1 (MQ=255)
                ggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCAGCGTTACTTATTTTTATCGGCGTGTTTATTTTCCTCGTACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTggg                                                                                                                           <  7:878318/140‑1 (MQ=255)
                ggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTggg                                                                                                                           <  1:696720/140‑1 (MQ=255)
                      cgcTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTGCTCGGGCGCAAACAGTCACGCGCCGGGCCTCTGGG‑GCGCGTCGGGTTTGGGGTTgggggggtt                                                                                                                     >  1:350396/1‑134 (MQ=255)
                      cgcTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTCATCGGACCCAAACAGTAACGCGCCGGGCAACTGGG‑CCGCGGCGGGTTTTGTATTGGGCAGAtt                                                                                                                     >  7:484155/1‑140 (MQ=255)
                      cgcTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGGATTGGTCTTGGGCTGAtt                                                                                                                     >  4:107986/1‑140 (MQ=255)
                      cgcTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTAGGACGCAAACAGTCACGCGCCGGGCCACTGGG‑CCGCGTGGGGTTTGGTGTTGGGCTGttt                                                                                                                     >  3:827086/1‑140 (MQ=255)
                          gATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTAc                                                                                                                 <  6:988608/140‑1 (MQ=255)
                          gATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTAc                                                                                                                 <  3:307326/140‑1 (MQ=255)
                           aTGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACt                                                                                                                <  5:983846/140‑1 (MQ=255)
                           aTGGCGCGTATTCTCACCTTAGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACt                                                                                                                <  5:672790/140‑1 (MQ=255)
                             ggCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTgg                                                                                                              <  3:673016/140‑1 (MQ=255)
                               cgcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGgcg                                                                                                            <  4:894336/140‑1 (MQ=255)
                               cgcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGgcg                                                                                                            <  3:107986/140‑1 (MQ=255)
                               cgcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGgcg                                                                                                            <  4:841640/140‑1 (MQ=255)
                                gcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGgcgc                                                                                                           <  2:340290/140‑1 (MQ=255)
                                 cgTATTCTCACCTTCGATTTATACTGGCTGTCACCGTTACTTATTTTTATCCGCGTGATTATTTTCAACGGCCGCAAACACTCCCGCGCCGGGCCCCTGGGCCCGCG‑CGGTTATGGGTGTGGGGGGCTTTTACTGgcgcg                                                                                                          >  5:917620/1‑139 (MQ=255)
                                    aTTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTTGGCTGATTTTACTGGCGCTGGa                                                                                                       <  4:22151/140‑1 (MQ=255)
                                    aTTCTCACCTTAGATTTATCCTGGCTGTCTCCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCAGGTCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGa                                                                                                       <  8:138904/140‑1 (MQ=255)
                                      tatcACCTTCGATTTATCCTGGCTGTCATCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGt                                                                                                     <  3:3450/138‑1 (MQ=255)
                                        tcACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTg                                                                                                   <  2:593023/140‑1 (MQ=255)
                                         cACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGa                                                                                                  <  2:44002/140‑1 (MQ=255)
                                         cACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGa                                                                                                  <  6:295580/140‑1 (MQ=255)
                                         aaCCTTCGATTTATCCTGGATGTCACAGTTACTTTTTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGa                                                                                                  <  8:748314/139‑1 (MQ=255)
                                          aCCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGGAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGCTTTTACTGGGGCGGGAGGTGAt                                                                                                 >  3:881352/1‑140 (MQ=255)
                                          aCCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTGCTCGGCCGCAAACAGTCACGCGCCCGGCAACTGGG‑CCGCGTGGGTATTTGTGGTGGGCGGATTTTAAGTGCGCTGGTGTTTAt                                                                                                 >  7:71150/1‑140 (MQ=255)
                                          aCCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGCCGCAAACCGTCACGCGCCCGGCAACTGGG‑CCGCGGCGGGATTGGTCTTGGGCTGATTTTACTGGCGCTGGTGTTGAt                                                                                                 >  5:881971/1‑140 (MQ=255)
                                          aCCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGAt                                                                                                 >  2:523486/1‑140 (MQ=255)
                                                gATTTATGCTGGCTGTCACCGTTACTTATTTTTTTCGTCGGGATTTTTTTCCTCGTACGCAAACGCTCACGCGCCTGGCAACTGGG‑CCGCGTCGGTATTGGTCTTTGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCa                                                                                           <  1:90759/140‑1 (MQ=255)
                                                gATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCa                                                                                           <  2:601547/140‑1 (MQ=255)
                                                gATTTATCCTGGCGGGCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCa                                                                                           <  1:858901/140‑1 (MQ=255)
                                                gATTTATCCTGGCAGTCACCGTTACTTATTTTTCTCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCa                                                                                           <  2:1029300/140‑1 (MQ=255)
                                                  tttATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAgg                                                                                         <  5:176947/140‑1 (MQ=255)
                                                        cTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTaa                                                                                   <  7:711232/140‑1 (MQ=255)
                                                            cTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGcc                                                                               <  6:812254/140‑1 (MQ=255)
                                                                cACCGTTACTTATTTTTATCGGCGTGATTTTTTTAATCGGACGCAAACAGTAACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTTGTGTTGGGGTGATTTTTCTGGGGCTGGTTTTGATTGTGCAGGCCGTAACGCCGAtg                                                                           >  2:361835/1‑139 (MQ=255)
                                                                 aCCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCa                                                                          <  1:523486/140‑1 (MQ=255)
                                                                   ctttACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACt                                                                        <  2:387022/138‑1 (MQ=255)
                                                                   cGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACt                                                                        <  8:803691/140‑1 (MQ=255)
                                                                       aCTTATTTTTATCGGCGTGATTTTTTTCCTAGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTTTTGGTCTTGGGGTGATTTTTATGGGGCGGGAGTTGATTTTTCAGGCCGTAAAGCCGCTCACACAgg                                                                    >  2:949011/1‑140 (MQ=255)
                                                                       aCTTATTTTTATCGGCGTGATTTTTTTCCGCGGACGCAAACAGTAACGCGCCGGGCAACTGGG‑CCGCGTGCGGATTGGTGTTGGGGTTGATTTACTGGAGAATGATTTTATTGTGCAGGCCCTAAAGACGAAAACTAAgg                                                                    >  2:228676/1‑140 (MQ=255)
                                                                           ttttttATCGGGGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTAGTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                <  3:605198/139‑1 (MQ=255)
                                                                           attattATCGCCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCTGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGTATGTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                <  5:600616/140‑1 (MQ=255)
                                                                           aTTTTTATCTGAGTGATTTTTTTCCTCGGACGCAAACCGTCACGCGCCGGGCAACGGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                <  3:635987/140‑1 (MQ=255)
                                                                           aTTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                <  2:1118725/140‑1 (MQ=255)
                                                                                tATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGGCGGTATTGGTGTTGGGGTGAATTTTATGGGGCTGGAGTTGTTTGTGCCTGCCGTAAAGCCCATCACACAGGCAAACGGCg                                                           >  8:891333/1‑140 (MQ=255)
                                                                                   cGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTc                                                        <  4:419343/140‑1 (MQ=255)
                                                                                      cGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAgg                                                     <  1:396808/140‑1 (MQ=255)
                                                                                              ttttCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCttt                                             <  6:60461/140‑1 (MQ=255)
                                                                                                    tCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCg                                       <  3:718724/140‑1 (MQ=255)
                                                                                                    tCGGACGCAAACAGGCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGGCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCg                                       <  7:240118/140‑1 (MQ=255)
                                                                                                        aCGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGa                                   <  3:184596/140‑1 (MQ=255)
                                                                                                            aaaCAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTgg                               <  5:492844/140‑1 (MQ=255)
                                                                                                            aaaCAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTgg                               <  3:151765/140‑1 (MQ=255)
                                                                                                             aaCAGTCACGTGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGc                              <  5:118267/140‑1 (MQ=255)
                                                                                                                 gTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACa                          <  8:949685/140‑1 (MQ=255)
                                                                                                                  tCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGAATGCCGTAACGCCGATAACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACAt                         >  7:1007710/1‑140 (MQ=255)
                                                                                                                    aCGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACAttt                       >  8:720938/1‑140 (MQ=255)
                                                                                                                       cgcCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATGTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCCAACGGCGTTCAGGTGGTATTTGCCTCGCTGACTGGGGACATTTTgg                    >  5:419265/1‑139 (MQ=255)
                                                                                                                          ggggCTACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTgg                 <  4:606982/139‑1 (MQ=255)
                                                                                                                          cGGGCAGCGGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTgg                 <  2:108537/140‑1 (MQ=255)
                                                                                                                          cGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTgg                 >  6:419104/1‑140 (MQ=255)
                                                                                                                          cGGGCAACTGGG‑CCGCGTCGGTATTGCTCAAGGGCAGCTTTTACTGGCGCAGGTGATGATTGAGCAGCCCGTAACCCCCATCACACAGGCAAACGGCGTTCAGGGGATCATTGCCTCGCTGACTGGCGACAATTTGCTgg                 >  2:161402/1‑140 (MQ=255)
                                                                                                                            ggCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGAt               >  8:401679/1‑140 (MQ=255)
                                                                                                                            ggCAACTGGG‑CCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGAt               <  1:1047378/140‑1 (MQ=255)
                                                                                                                            ggCAACTGGG‑CCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGAt               >  4:182756/1‑140 (MQ=255)
                                                                                                                            ggCAACTGGG‑CCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCGGGAGTTGATTGTGCCGGCCGTAACGCCGGGCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGGTGACTGGGGACATTTTGCTGGAt               >  2:464486/1‑140 (MQ=255)
                                                                                                                               atcTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCTCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg            <  3:507603/138‑1 (MQ=255)
                                                                                                                               aaCTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg            <  5:80031/140‑1 (MQ=255)
                                                                                                                               aaCTGGG‑CCGCGTCGGTATTGGGCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg            <  7:124336/140‑1 (MQ=255)
                                                                                                                                 cTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCt          >  6:644453/1‑140 (MQ=255)
                                                                                                                                   ggg‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGa        <  7:393955/140‑1 (MQ=255)
                                                                                                                                   ggg‑CCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGa        <  8:342441/140‑1 (MQ=255)
                                                                                                                                    gg‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGAt       <  2:1070400/140‑1 (MQ=255)
                                                                                                                                    gg‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGAt       <  1:668591/140‑1 (MQ=255)
                                                                                                                                    gg‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGAt       <  6:948722/140‑1 (MQ=255)
                                                                                                                                          cgTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGa                                                                                                  >  4:330461/1‑44 (MQ=255)
                                                                                                                                          cgTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTTCTGGATGCGCTGATTGGTg  >  5:698745/1‑140 (MQ=255)
                                                                                                                                          cgTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTg  <  6:281633/140‑1 (MQ=255)
                                                                                                                                          cgTCGGTATTGGTCGTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACCGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTg  >  7:614375/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                         
CTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGTCACGCGCCGGGCAACTGGG‑CCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATTGTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTG  >  NZ_CP022686/3020090‑3020366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A