Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I0 R1 235 6960.4 9135780 98.4% 8989607 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 3,020,229 T→G 15.9% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226863,020,2290TG15.9% 282.5 / 10.3 178V133G (GTC→GGC) yjbBNa/Pi cotransporter family protein
Reads supporting (aligned to +/- strand):  ref base T (40/105);  new base G (25/8);  total (65/113)
Fisher's exact test for biased strand distribution p-value = 4.62e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

CTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTGCG  >  NZ_CP022686/3020090‑3020368
                                                                                                                                            |                                                                                                                                            
cggggTGCAGATGTCGGGCCGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTATCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGt                                                                                                                                              <  7:875180/138‑1 (MQ=255)
cTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGt                                                                                                                                              <  2:408389/140‑1 (MQ=255)
cTGGGTGCAGATGGCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGt                                                                                                                                              <  1:492906/140‑1 (MQ=255)
cTGGGTGCAGATGGCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGt                                                                                                                                              <  8:141552/140‑1 (MQ=255)
cTGGGGGCAGATGGCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGt                                                                                                                                              <  5:642095/140‑1 (MQ=255)
  gggTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCGAGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCg                                                                                                                                            >  2:543143/1‑140 (MQ=255)
   ggTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGTTTTTTTTAATCGGCCGCAAACAGG‑CCAGCGCCGGGCAAAAGTGCCGCGTggg                                                                                                                                           >  7:565125/1‑140 (MQ=255)
   ggTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCgg                                                                                                                                           >  8:962211/1‑140 (MQ=255)
   ggTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCgg                                                                                                                                           >  5:722015/1‑140 (MQ=255)
      gctgttGTTGGGACGGCGCTGATTGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCTTTACTTATTTTTATCGGTGTGATTTTTTTCCTAGGACGCAAACATT‑CACGCGCCGGTCAACTGGGCCGCGTCTGt                                                                                                                                          <  4:1133181/133‑1 (MQ=255)
      gctgATGTGGGGACGGCGCTGATGTCGAGTATTCTCCAGTTAGATTTATACTGGCTGTCACCGTTACTTTTTTTTATCGTCGTGATTTTTTTCCTCGGACGCAAACCGT‑CACGCGCCGGGCTACTGGGCCGCGTCgtt                                                                                                                                          <  3:205070/135‑3 (MQ=255)
      gCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTAt                                                                                                                                        <  1:685372/140‑1 (MQ=255)
      gCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTAt                                                                                                                                        <  7:321168/140‑1 (MQ=255)
        aGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTGCTCGGGCGCAAACAGT‑CCCGCGGCGGGGCACAGGGGCCCGGCGGGATTg                                                                                                                                      >  1:423451/1‑140 (MQ=255)
        aGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATACTGGCTGTCCACGGTACTTATTTTTATCGGCGTGTTTTTTTTACTCGGTCGCAAAACCT‑AACGCTCCGGGCAACTGGTCCGCGTGGGTAttt                                                                                                                                      >  2:376687/1‑139 (MQ=255)
          aTGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGt                                                                                                                                    >  8:1017189/1‑140 (MQ=255)
           tGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTc                                                                                                                                   <  7:239795/140‑1 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCCAACAGT‑CACCCGCCGGGCACCTGGGGCGGGGCGGGTTTGGtggtgg                                                                                                                               >  6:225377/1‑135 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACACT‑CAAGCCCCGGGCAACTGGGCCGCGGCGCTATTGGGGTTgg                                                                                                                               >  2:801185/1‑140 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTCGGACGCAAAAAGT‑CAAGCGCCGGGCCACAGGGCCGCGTGGGGATTGGTCTTgg                                                                                                                               >  5:397306/1‑140 (MQ=255)
               gggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGAATTTTTTACTCGGACGCAAACCAT‑CACGCGCCGCGCAACTGGGCCCCGTCGGtcttggtcttgg                                                                                                                               >  6:908192/1‑140 (MQ=255)
                ggACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGTCGTTATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTggg                                                                                                                              <  2:404649/140‑1 (MQ=255)
                 gACGGCGCTGATGGCGCGAATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTGTTgg                                                                                                                               >  4:305985/1‑138 (MQ=255)
                  aCGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGTTTTTTTTCCGCGGCCGCCAACAGT‑AACGCGCCGGGCAACAGGGCCGCGGGGGGTTTGGTGTTGGGcg                                                                                                                            >  6:1069917/1‑139 (MQ=255)
                    ggCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGTTTTTTTTTCTAGGACGCAAACAGT‑CACCCGCCGGGCCTCTGGGCCGCGGCGGGATTGGTCTTGGGGTGa                                                                                                                          >  1:190636/1‑140 (MQ=255)
                      cgcTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCCCCGTTACTTATTTTTATCGGCGTGATTTTTTTGGTCGTGCGCAAAAAATACAC‑CGCCGCGCAACTGGGGCGCGGCGGGATTGGTGTTtgggttgtt                                                                                                                        >  7:40186/1‑134 (MQ=255)
                      cgcTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTGCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGGATTGGGGTTGGGGTGAtt                                                                                                                        >  7:902664/1‑140 (MQ=255)
                        cTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGTTTTTTTTACTCGGACGCAAACAGT‑CACGCGCCGGGCAACAGGGCCGCGGGGGTTTTGGGGTTGGGCTGAtt                                                                                                                        >  3:380938/1‑138 (MQ=255)
                         tGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGTTTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCCACTGGGCCGCGGCGGTATTGGTGTTTGGCTGAttt                                                                                                                       >  4:239683/1‑138 (MQ=255)
                             ggCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGGCGGTATTGGTCTTGGGCTGATTTTACTgg                                                                                                                 <  8:993123/140‑1 (MQ=255)
                               ggcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGgcg                                                                                                               <  5:543287/139‑1 (MQ=255)
                               cgcgTATTCTCACCTTCGATTTATCCTGGCTGGCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGgcg                                                                                                               <  2:1163155/140‑1 (MQ=255)
                               cgagTATTCTAAACTTCTATTTCTCCTGGATGTTACCGTTACTTATTTTTATCGTCGTGATTTTTTTACTATGAAGAAAACAGT‑CACGCGCCTGTCAACTGGGCCGCGTCGTTATTGGTCTTGTGCTGATTTTACTggag                                                                                                               <  6:670733/137‑3 (MQ=255)
                                gcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTGCTAGGCCGCAACACGT‑GACGCGGCGGGCAACTTGGCCGCGTGGGGATTGGTCTTGGGGTGTTTTTAAGGgcgg                                                                                                              >  1:717224/1‑139 (MQ=255)
                                gcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CCCCCGCCGGGCAACTGGGCCGCGGCGGGATTGGTATTGGGGTGATTTTACTTgcgc                                                                                                              >  8:897072/1‑140 (MQ=255)
                                gcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTTGGCTGGTTTTACTGgcgc                                                                                                              >  8:41385/1‑140 (MQ=255)
                                gcgTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTACGACCCAAAAACT‑CACACGCCGCGGAACAGGGCCCCGGACGGATTGGTCTTGGGGGGATTTTAATTgcgc                                                                                                              >  2:743097/1‑140 (MQ=255)
                                    aTTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGa                                                                                                          <  7:460985/140‑1 (MQ=255)
                                    aTTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGa                                                                                                          <  1:944339/140‑1 (MQ=255)
                                    aTTCTCACCTGCGATTTATCCTGGCTGTCACCGTTACTTATTTTTCTCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGa                                                                                                          <  7:659265/140‑1 (MQ=255)
                                      tttctccTTCGATTTATAATGGCTGTCACCGTTTCTTATTTTTATCGTCGTGATTTTTTTCTTGGGACGCATACAGT‑CACGCGCCGGGCTACTGTGCCGCGGCGGTATTTGTCTTGGGCTGATTTTACTTGCGCTGTAGt                                                                                                        <  8:551970/135‑1 (MQ=255)
                                      tctcACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGt                                                                                                        <  5:1033310/140‑1 (MQ=255)
                                         cACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGtt                                                                                                       <  4:959304/138‑1 (MQ=255)
                                         cACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGtt                                                                                                       <  4:1000509/138‑1 (MQ=255)
                                         cACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGtt                                                                                                       <  3:834181/138‑1 (MQ=255)
                                         cACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGa                                                                                                     <  6:722015/140‑1 (MQ=255)
                                            cTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGAtttt                                                                                                                      >  4:1124661/1‑120 (MQ=255)
                                            cTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGAtttt                                                                                                                      <  3:1124661/120‑1 (MQ=255)
                                              tCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTTATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATt‑gtg                                                                                               <  7:744016/140‑1 (MQ=255)
                                              tCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATt‑gtg                                                                                               <  6:502017/140‑1 (MQ=255)
                                               cGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGa                                                                                                     >  7:338042/1‑134 (MQ=255)
                                                gATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCa                                                                                             <  7:937950/140‑1 (MQ=255)
                                                gATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCa                                                                                             <  2:140471/140‑1 (MQ=255)
                                                  tttATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAgg                                                                                           <  1:622056/140‑1 (MQ=255)
                                                       ccaggCTGACACAGGAACTTATTATTAGCGGGTTGATTGTTTTCCACGGACGCAAACAGA‑CCCGGGCCTTGCATCGGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCTCTGGAGTTGATT‑TTGCAGGCCGTa                                                                                      <  8:281314/137‑1 (MQ=255)
                                                       ccTGGCTGTCACCGTTACTTATTTATATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTa                                                                                      <  2:245375/140‑1 (MQ=255)
                                                       ccTGGCGGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTa                                                                                      <  1:595385/140‑1 (MQ=255)
                                                        cTGGCTGTCACCGTTACTTATTTTTATCGGCGTGGTTTTTTTCCTGGGACGCCAACAGT‑CACGCGCCGGGCCAATGGGCCGCGGCCGTTTTGGTGTTTGGGGGATTTTTATGGCGCGGGGGTTGATT‑GTGGAGGCCGt                                                                                       >  3:735882/1‑138 (MQ=255)
                                                           gCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGCCCCAAACAGT‑CCCCCGCCGGGCAACGGGGCCGCGGCGGTTTTGGGGTTGGGGGGATTTTAGTGGGGCGGGGGTTGTTT‑GGTCCGGCCGTAACGc                                                                                  >  5:128857/1‑140 (MQ=255)
                                                           gCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTAGGACGCAAACAGT‑CACCCGCCGGGCCACTGGGCCGCGTCGGGTTTTGTGTTGGGGTGAATTTAAGGGCGCGGGAGTTGATT‑TTGCAGGCCGTAAccc                                                                                  >  1:99231/1‑138 (MQ=255)
                                                            cttgcACCGTTAATTATTTTTATCGTCTTTATTTTTTTCATCGGACGAAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGcc                                                                                 <  2:883087/136‑1 (MQ=255)
                                                            cTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGcc                                                                                 <  1:876261/140‑1 (MQ=255)
                                                            cTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGcc                                                                                 <  5:208710/140‑1 (MQ=255)
                                                             tGTCACCGTTCCTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCGGGAGTTGATT‑GTGCAGGCCGTAACGCCg                                                                                <  6:214702/140‑1 (MQ=255)
                                                             tGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGGCGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGGATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGc                                                                                  >  4:746107/1‑138 (MQ=255)
                                                               tCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGAt                                                                              <  1:743097/140‑1 (MQ=255)
                                                                cACCGTTACTTATTTTTATCGGCGTGATTTTTTTACTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACAGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCCATc                                                                             >  8:806205/1‑140 (MQ=255)
                                                                   cGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACt                                                                          <  7:169943/140‑1 (MQ=255)
                                                                   cGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACt                                                                          <  7:108012/140‑1 (MQ=255)
                                                                   cGTTACTTATTTTTATCGGCGTGATTTTTTTCCAAGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGGAGGTATTGGTGTTGGGCTGATTTTAATGGCGCGGGAGTTTATTGGTGC‑GCCCCTTACGCCGATcc                                                                            >  4:295162/1‑137 (MQ=255)
                                                                           aTTTTTATCGGCGTGATTTTTTTCCTCGGACGCACACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                  <  6:580617/140‑1 (MQ=255)
                                                                           aTTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CCCGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                  <  6:846295/140‑1 (MQ=255)
                                                                           aTTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                  <  8:519271/140‑1 (MQ=255)
                                                                             ttttATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                  <  4:838153/138‑1 (MQ=255)
                                                                             ttttATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCaaa                                                                  <  4:235144/138‑1 (MQ=255)
                                                                                tATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACgg                                                               <  3:1055265/138‑1 (MQ=255)
                                                                                tATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCg                                                             <  1:1071936/140‑1 (MQ=255)
                                                                                  tCGGCGTGATTTTTTTCCTCGGCCGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGtt                                                           <  5:669427/140‑1 (MQ=255)
                                                                                  tCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCg                                                             <  3:581770/138‑1 (MQ=255)
                                                                                  tCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGtt                                                           <  5:900526/140‑1 (MQ=255)
                                                                                      cgggATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCATTGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAgg                                                       <  6:243137/137‑1 (MQ=255)
                                                                                      cGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAgg                                                       <  5:163117/140‑1 (MQ=255)
                                                                                      cGTGATTTTTTTCCTCGGACGCAAACAGG‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAgg                                                       <  6:381136/140‑1 (MQ=255)
                                                                                                                  t‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACAt                           >  1:835952/1‑140 (MQ=255)
                                                                                                                  t‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACAt                           <  1:332472/140‑1 (MQ=255)
                                                                                                                     aCGCGCCGGGCAACTGGGCCGCGTCGGTTTTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCCCTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACAttt                         >  8:841882/1‑140 (MQ=255)
                                                                                                                     aCGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACAttt                         >  5:411924/1‑140 (MQ=255)
                                                                                                                           cGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTgg                   <  5:951375/140‑1 (MQ=255)
                                                                                                                             ggCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGAt                 >  5:24596/1‑140 (MQ=255)
                                                                                                                             ggCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGGTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTgg                   >  3:347271/1‑138 (MQ=255)
                                                                                                                             ggCAACTGGGCCGCGTCGGTATTGGTCGTGGGCTGATTTTACGGGCGCGGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATATTTGCCTCGCTGACTGGCGACAGTTTGCTGGAt                 >  5:810660/1‑140 (MQ=255)
                                                                                                                             ggCAACTGGGCCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGGTCAGGTGATCTTTGCCTCGCTGACTGGCGCCATTTTGCTGGAt                 >  1:117105/1‑140 (MQ=255)
                                                                                                                                aaCTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGCTT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg              <  8:167089/140‑1 (MQ=255)
                                                                                                                                aaCTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg              <  5:265097/140‑1 (MQ=255)
                                                                                                                                aaCTGGGCCGCGGCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg              <  1:574659/140‑1 (MQ=255)
                                                                                                                                  cTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATgcg              <  4:1085658/138‑1 (MQ=255)
                                                                                                                                    gggCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCt            <  3:376054/138‑1 (MQ=255)
                                                                                                                                     ggCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGAt         <  2:119570/140‑1 (MQ=255)
                                                                                                                                     ggCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAAAGCCGATCTCTCAGGCAAACGGCGTTCAGGTGATCTTTGCATCGCTGACTGGCGACATTTTGCTGGATGCGCTGAt         <  2:1112913/140‑1 (MQ=255)
                                                                                                                                          cgTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTg    <  1:431313/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CTGGGTGCAGATGTCGGGACGGCGCTGATGGCGCGTATTCTCACCTTCGATTTATCCTGGCTGTCACCGTTACTTATTTTTATCGGCGTGATTTTTTTCCTCGGACGCAAACAGT‑CACGCGCCGGGCAACTGGGCCGCGTCGGTATTGGTCTTGGGCTGATTTTACTGGCGCTGGAGTTGATT‑GTGCAGGCCGTAACGCCGATCACTCAGGCAAACGGCGTTCAGGTGATCTTTGCCTCGCTGACTGGCGACATTTTGCTGGATGCGCTGATTGGTGCG  >  NZ_CP022686/3020090‑3020368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A