Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F35 I0 R1 157 6985.9 8006972 98.3% 7870853 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,056,962 C→A 15.0% F137L (TTC→TTA)  adk → adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,056,9620CA15.0% 171.4 / 3.3 114F137L (TTC→TTA) adkadenylate kinase
Reads supporting (aligned to +/- strand):  ref base C (15/74);  new base A (14/8);  total (32/82)
Fisher's exact test for biased strand distribution p-value = 3.25e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

AGGCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTA  >  NZ_CP022686/4056826‑4057093
                                                                                                                                        |                                                                                                                                   
aggcagACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCa                                                                                                                                    <  2:206009/138‑1 (MQ=255)
    agACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATcc                                                                                                                                >  5:283237/1‑138 (MQ=255)
    agACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATAACGTTTAATTCAAtac                                                                                                                                >  6:289399/1‑136 (MQ=255)
       cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGTGCGTGTTTATCACGTTAAATTCAATcagcc                                                                                                                             <  5:664249/138‑1 (MQ=255)
       cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATccgcc                                                                                                                             <  5:588113/138‑1 (MQ=255)
       cgcgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACAGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGa                                                                                                                           <  8:823034/140‑1 (MQ=255)
       cgcgATGAAAGAAGCGGGCATCAAGGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGa                                                                                                                           <  8:664958/140‑1 (MQ=255)
         cgATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGa                                                                                                                           >  4:572000/1‑138 (MQ=255)
            tGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCT‑GTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGt                                                                                                                       <  3:133407/138‑1 (MQ=255)
                   tgCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTTATTGTTGACCGTATGGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAgg                                                                                                                 <  5:744770/137‑1 (MQ=255)
                     ggggCATCAATGTTGATTACGTTCTGGAATTCTCCGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGGGTTCACGCGCCGTCGGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCa                                                                                                               <  4:600984/137‑1 (MQ=255)
                     cGGGCTTCAATGTTGATTACGTTCTGTATTTCTACGTACGGGACTAACTTATTGGTGACGTTATCGTTGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTAACTCGTTAAATTCAATCCGCCGAAAGTAGAAGGCaaa                                                                                                             <  8:152258/140‑1 (MQ=255)
                      gggCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTCACGGTTAATTAAATCCGCCGAAAGTAGACGGCaaac                                                                                                            >  7:810717/1‑139 (MQ=255)
                      gggCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCAGGCTGGTCGTGTTATTGACCTTAAATTAAAAACCCCGAAAAAAGAAGGCaa                                                                                                              >  2:459786/1‑138 (MQ=255)
                        gcctCAATTTTGATTAGGTTCTGTATTTCGACGTCCCGTACTAACTGATTTTTCACCTTATCTTAGGCCGCCGCGTTCACTGGCTGTCTGTTCGTGTTTTTCTCGTTAAATTCAATCCGCCGAAATTAGAAGGaaatg                                                                                                            <  2:101158/135‑3 (MQ=255)
                        gCATCAATTGTTATTACGTTCTGTAATTGGACGTCCCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAg                                                                                                            <  5:403403/138‑1 (MQ=255)
                        gCATAATTGTTGATTACGTTGTGGATTTCGAGTTACCGGACGAAATGATTGTTGACCTTATCGTAGGCCGCGGCGTTCACGCGCCGGTTGGTGTTGTTTATCACGTTAAATTCAATCCGCCCAAATTAGAAGCCAAAg                                                                                                            <  2:819953/138‑1 (MQ=255)
                                tttGATTACGTTATGGAATTCGACGTCCCGTACTAACTGATTGTTGACCGTATCGTCGGCCGCCGCGGTCCCGCGCCGGCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAACGTCGAAGGCAAAGACGACGtt                                                                                                    <  6:341763/137‑1 (MQ=255)
                                gtggATTTCGTTCTGTAATTGGACGTACCGTACGAACTTATTGTTGACTGTTTCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTAGTGTTTATCACGTTAAATTCAATCCGCCGAATGTATAAGTCAAAGACGACGtt                                                                                                    <  4:155717/135‑1 (MQ=255)
                                    aTTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATAACCGTAAATTCAATACGCCGAAACGAGAAGCCAAAAGCGCCGTTACCggg                                                                                              >  7:388993/1‑139 (MQ=255)
                                     ttACGTTATGGAATTCGACGTACCGGACGAATTGATTGTTGACGGTATCGTAGGCCGCCGCTTTCACAGGCCGTCTGGGCTTGTTTATCACGTTAAATTCATTCCGCCGAAAGTAGAAGGAAAAGACGACGTTACCgg                                                                                               <  6:691617/138‑1 (MQ=255)
                                     taaCGTTTTTGAATTCGACGTACCGTCCGAAATGATTGTTGACCGTATCTTAGGCCGCCGCGTTCACGCGCCGTCTGGTGGTGTTTATCACTTTAAATTCAATCCGCCTAAAGTAGATGGCAAAGACGACGTTACCgg                                                                                               <  6:722449/136‑1 (MQ=255)
                                        cGTTCTGGAATTCGCCGTACGGCACGAACTTATTTGTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTCACGTTAAATTCAATCCGCCGAAAGTAGATTGCAAACACGACGTTACCGGTga                                                                                            <  6:953396/138‑1 (MQ=255)
                                        cGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGGTAAATTCAATCCGCCGAAAGTAGAATGCAAAGACGACGTTACCGGTga                                                                                            >  4:798882/1‑138 (MQ=255)
                                         gTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGACGCGTTCACGCGCAGTCTGGTCGTGTTTATCACGTTAAATTAAATACGCCGAAAATAGAAGGCAAAGACGACGTTACCGGTgaaga                                                                                         >  8:832322/1‑140 (MQ=255)
                                            cTGGAATTCGACGTACCGGACGAAATGATTGTGCACCGTAGCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGGGTTTATCACGTTAAATACAATCCGCCGAAAGTAGAAGCCAAAGACGACGTTACCGGTGAAGAACt                                                                                      <  8:375058/140‑1 (MQ=255)
                                               gAATTCGACGTACCGGCCGAACTGATTGTTCACCGTTGCGTTGGCCGCCGCGTTCACGCGCCGTCTGGTGGTGTTTATCACGTAAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTg                                                                                     <  5:645575/138‑1 (MQ=255)
                                                aaTTCTACGTAGCGGACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGGGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGAAAAGACGACGTTACCGGTGAAGAACTGa                                                                                    <  6:387768/138‑1 (MQ=255)
                                                aaTTCGACGTACGGCACTAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCTTGTTTATCACGTTAAATTCAATCCGCCCAATGTAGAAGGAAAAGACGACGTTACCGGTGAAGAACTGa                                                                                    <  3:928358/138‑1 (MQ=255)
                                                aaTTCGACGTACCGGACTAACTGATTGTTGACCTTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGa                                                                                    <  1:344497/138‑1 (MQ=255)
                                                aaTTCGACGTACCGGACGAACTGATTGTTGACCGTATCTTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGAAAAGACGACGTTACCGGTGAAGAACTGACt                                                                                  <  7:850782/140‑1 (MQ=255)
                                                    cGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATGACGTTAAAATGAATACGGCGCAACTTGACTGCAAATACGCCGTTACCGGGGAACAAATgcacaccc                                                                              >  7:814674/1‑133 (MQ=255)
                                                        gTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTTAATTCAATCCGCCGAAAGTATAAGGCAAAGCCGACGTTACCGGTGAAGAACTGCCTACCCGt                                                                            >  5:435925/1‑138 (MQ=255)
                                                        gTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGGTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCCGTGAAGAACTGACTAccccg                                                                            >  1:550139/1‑136 (MQ=255)
                                                         tACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTTTAACGTTAAATTAAATACCCCGAAAGTAGAAGGCAAAGACCACGGTTCCGGTGAATAACAGACTACCCGTa                                                                           >  3:400040/1‑138 (MQ=255)
                                                           ccGGAGGACCTGTTTGTTGACGGTATGGTTGGCCGCGGCGTTCTCGCGCCGTGGGGTCGTTTTTATCAGTTTAATTTAAATCCGACTAAAGTATATGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTaaa                                                                         <  3:516227/138‑1 (MQ=255)
                                                           ccGGACTAATTGATTGTTGACCTTAGCGTAGGCCGCCGCGGTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAATGTCGAAGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTaaa                                                                         <  6:22392/138‑1 (MQ=255)
                                                               ccGAACTTTTTGTTGCCCGTATCGTAGGCCGCCGCGTGCACGCGCGGTCTGGTGGTGTTTATCAGGTTAATTTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  5:8994/137‑1 (MQ=255)
                                                               ccGAAATGATTGCTCACCGTACCGTAGGCCGCCGGGTACACGCGCCGTATGGGCGTGTTTATCACGTAAAATTCAATCCGCCGAAAGTATATGGCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  5:559206/137‑1 (MQ=255)
                                                               actaaCTGATTGTTGACCGTCTGGTCGGCGGCGGCTTTCACGCGCGGGCGGGTCGGGTTTATCACGTTAAATTCAATCCCCCTAAAGTAGAAGCCAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  1:416295/135‑1 (MQ=255)
                                                               actaaCTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTTTTTATCACGTTAAATTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  4:32123/135‑1 (MQ=255)
                                                               actaaCTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  3:674175/135‑1 (MQ=255)
                                                               aCGAACTTTTTGTTGACCGTATCGTTGGCCGCGGCGTTCACGCGCCTTCTGGTCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  5:877236/138‑1 (MQ=255)
                                                               aCGAACTTATTGTTGACCGTATCGTTGGCCGCCGCTTGCACGCGCCGTGTGGTCGTTTTTATCAGGTTAAATTCAATCCGCCGAATGTAGACGCAAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  3:403615/138‑1 (MQ=255)
                                                               aCGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  6:283237/138‑1 (MQ=255)
                                                               aCGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGGTCACGCGCCGTCTGGGCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  4:534451/138‑1 (MQ=255)
                                                               aCGAACTGATTGTGGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGGCGGGTTTATCCCGTTAAATTCAATCCGCCGACAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACg                                                                     <  2:554719/138‑1 (MQ=255)
                                                                ctaaCTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCGGTCTGGGCGTGTTTAACACTTTAAATTCAATCGGCCGAAAGTACAAGGAAACGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGa                                                                    <  5:36673/136‑1 (MQ=255)
                                                                    cTGATTGTTGACCGTATCTTAGGCCGCGGCGTTCACGCGCGGTCTGGTCGTGTTTATAACGTTAAATTCAATCCGCCCAAAGTAGATGGCAAACACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAg                                                                <  3:135189/138‑1 (MQ=255)
                                                                       tttgtggACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGGGGTTTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAaga                                                           <  8:219534/139‑1 (MQ=255)
                                                                        ttgttgACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATCACGTTAAATTCAATCCGCCCAAATTAGAAGGCAAAGACGCCGTTACACGTGAAAAACGGACGACCAGTAAACACGAACAGGAag                                                            >  4:74812/1‑138 (MQ=255)
                                                                           tttaCCGTATCGTCGGCCGCCGCGTTCACGCGCCGTCTGGTCGTTTTTATCACGTTAAATTCAATCCGCCTAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAgaga                                                         <  6:752391/135‑1 (MQ=255)
                                                                               ccGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACcgt                                                     >  1:19636/1‑138 (MQ=255)
                                                                                  aaTCGTAGGCCGCCGCGTGCACGCGCCGTCTGGGCGTGTTTATCCCGTAAAATTCACTCCCCCTAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAGCAGGAAGAGACcgtacgta                                                <  8:850287/139‑1 (MQ=255)
                                                                                   aTCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATAACGGTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACGGTACCGGTGAAGAACAGACTACCCGTAAAGACGATCAGGAAGCGACcgtacgt                                                 >  2:980701/1‑138 (MQ=255)
                                                                                   aTCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGGTTATCACGTTAAATTAAATACGCCGCAAGTAGAAGGGAAAGACGACGTTTCCGGGGAAGAACTGACTACCCGGAAAGACGATCAGGAAGAGACcgtaagt                                                 >  6:540100/1‑138 (MQ=255)
                                                                                       tAGCCCGCCGCGGTCACGCGCCGTCTGGTCGTGTTTATCCGTTTAAATTCAATCCCCCGAAAGTAGACGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGt                                           <  7:22297/140‑1 (MQ=255)
                                                                                        tggCCGCCGCGTTCACGCGCCGTCGGGTGGTGTTTATCAGGTTAAATTCAATCCGCCTAAAGTATAAGCAAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                            <  5:507445/137‑1 (MQ=255)
                                                                                        gggCCGCCGCTTACACGCGCGGTCTGGTTGTGTTTATCACTTTAAATTCAATCCCACGAACTTACAACGAAAAGACTACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGAACGTACGTAAACGTc                                          <  7:244501/139‑1 (MQ=255)
                                                                                        cggCCGCTGCTTTAAGGCGCCGTATGGTCGTGTTTATAACGTTAAATTCAAACCGCCTAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                            <  4:2948/137‑1 (MQ=255)
                                                                                        cggCCGCCGTGTTCACGCGCGGTCTGTTCGTTTTTATAATGTTAAATTCAATCCGCAGAAAGTAGAAGTCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                            <  1:619341/137‑1 (MQ=255)
                                                                                        cggCCGCCGCGTGCCCGCGCCGGATGGTCGGGTTTATCACGTTAACATCAATCCGCCGAAAGTAGCCGCAAAAGACGACGTTACCGGTGAAGAACTGACTCCCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                            <  2:612901/137‑1 (MQ=255)
                                                                                        cggCCCCCGCGGCAACGCGCGGTCTGGCGGTGTTAATCACGTTATATTCAACCCGCCGAACGTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACg                                            <  3:346023/137‑1 (MQ=255)
                                                                                            cgccgcGTTCACGCGCCGTCTGGTCGTGTTTATAACGGTAAATTCAATCCGCCGAAAGTATAAGGCAAAGACGACGTTACCGGTGAAGACCTGACTAACCGTAAATACGATAAGGAAGAGACCGTACGTAAACGtatt                                        >  2:874833/1‑135 (MQ=255)
                                                                                              ccgcGTTCACGCGCCGTCTGGTCGTGTTTATCACGTGAAATTAAAAACGCAGTAAGTATTAGGGAAAGACGACGTTAACGGTGGAGAAATGACGTACCGTAAAAAAGATCCTGAAGAAACCGTAAGTAAAAGTcagtg                                      >  2:937753/1‑134 (MQ=255)
                                                                                               cgcgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTGAATCCGGCGAAAGTTGAAGGCAAACACGGCGTTACCGGTGATGTACTGACGAGCCGTTAATACGATCAGGAAGAGTCCGTACGTAAACGTGAGGTTGa                                   >  8:939862/1‑140 (MQ=255)
                                                                                               cgcgTTCACGCGCCGTCTGGTCGTGTTTATACCGTTAAATTAAATCCGCCGAAAGTAGAAGGGAAAGCCGCCGTTACCCGGGAAGAACTGACTACCCGTAAAGACGCTAAGGAACAGACCGTAAGTAAACGTCGGGGTgt                                   >  8:939867/1‑139 (MQ=255)
                                                                                               cccgTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAATTTCATACCCCCGAAAGTAGAAGGCAACGACGACGTTACCTGTGAATAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGtt                                     <  5:317237/136‑1 (MQ=255)
                                                                                                gcgTTCACGCGCCGTCTGGTCGTGTTTTTAACGGTAAATTGAATACGCCGCAAGTAGAAGGCAAATACGACGTTACAGGTGAAGAACTGTCTACCCGGAAATACGCGGCTGAAGAGCCCGTACGGAAAAGTGAGGTTg                                    >  6:106753/1‑138 (MQ=255)
                                                                                                gcgTTCACGCGCCGTCTGGGCGTGTTTATCACGTAAAATTCATTCCCCCCAAAGTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGAGCAGGAAGAGACCGTACGTAAACGTCTGGTTg                                    <  4:670380/138‑1 (MQ=255)
                                                                                                 cgTTCACGCGCCGTCTGGTCTTGTTTATCACGTTAAATTCAATCCGCCTAAATTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGa                                   <  2:144395/138‑1 (MQ=255)
                                                                                                 cgTTCACGCGCCGTCTGGTCGTGGTTATAAAGTTAAATTAAATCCGCCGCAAGTAGAAGGCAAAGCCGCCGTGAACGGTGAAGAACTGACTCCCCCTAAAGCAGATCAGGAAGAGACCCTACCTAAACGGCTgctgta                                   >  6:973819/1‑133 (MQ=255)
                                                                                                 cgTTAAGGCTCCGTCTGGTCGTGTTTATCACGTAAAATTAATTCCGCCGAAATTAGAAGGCAAACACGACGTTACAGTAGAAGAAATGACTACCCCTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                 <  7:950153/140‑1 (MQ=255)
                                                                                                   ttCACGCGCGGTCTGGTCGTGTTTATCACGTTAAAAACAATCCGCCCAACGTAGACGCCAACGACGACGGTACCGGTGAAGAACTGAATACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAAt                                 <  4:249231/138‑1 (MQ=255)
                                                                                                      ccgcgcgGTTGGGGCGTGTTTATCACTTTAAATTCAATCCGCCGAAAGTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATAcc                              <  3:675634/137‑1 (MQ=255)
                                                                                                        gcgcCGTCTGGTCGTTTTTATCAGTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAACACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCAt                            <  1:928479/138‑1 (MQ=255)
                                                                                                        gcgcCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAACGTAGATGGCAAAGACGACGTTACCGGTGAAGAACTGACTACGCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCAt                            <  4:866803/138‑1 (MQ=255)
                                                                                                        gcgcCGGCTGGTCTTGTTAATCACGTTAAATTCAATCCGCCGAAAGTTGAAGCCAAAGACGTCGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCAt                            <  4:702323/138‑1 (MQ=255)
                                                                                                           ctgtgtGGTCGTGTTTATCACTTTAATTTAAATCCGCCTAATGTATACGGAAAAGACGACGTTACCGGTGAAGATATGACTACGCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                       <  7:367632/135‑1 (MQ=255)
                                                                                                           cggTCTGGTCGTGTTTATAACTTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAg                         <  1:874833/136‑1 (MQ=255)
                                                                                                           ccttatGGTCGTTTTTATCCCTTTAATTTATAACCGCCGAAAGTAGACGTCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGAAGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAg                         <  1:683032/133‑1 (MQ=255)
                                                                                                           ccGTGTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAATTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAg                         <  3:572000/138‑1 (MQ=255)
                                                                                                           ccGTCTGGTCTTGTTTATCACGTTAAATTCAATCCGCCTAAAGTATAAGGCAAAGACGACGTTGCCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTAAGTAAACGTCTGGTTGAATACCATCAg                         <  4:143264/138‑1 (MQ=255)
                                                                                                                tGGTCGTGTTTATCACGTTAAATTAAATCCGCCGAAAGGAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAACAGTCTGGTTGAATACCATCAGATGaa                    >  6:67400/1‑137 (MQ=255)
                                                                                                                tGGTCGTGTTTATCAAGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTAACCGGAAATACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCATATGaa                    >  2:600311/1‑137 (MQ=255)
                                                                                                                  gTCGTTTTATTCACTTTAAATTCAATCCGCCGAAAGTATAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTg                  <  3:298433/138‑1 (MQ=255)
                                                                                                                   tCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGc                 <  5:289399/138‑1 (MQ=255)
                                                                                                                     gtgtTTATCACGTTAAATTCAATCCGCCGAAAGTAGACGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCAc               <  2:729840/138‑1 (MQ=255)
                                                                                                                        tttATCACGTTAAATTCAATCCGCCGAAAGTACAAGCCAAAGACGACGTTACCGGTGAATAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGc            <  1:461531/138‑1 (MQ=255)
                                                                                                                         ttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAACACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGCCCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCt           <  4:529298/138‑1 (MQ=255)
                                                                                                                         ttATCACGTTAAATTCAAACCGCCGAACGTCGACGCCAAAGACGACGTTACCGGTGAAGAACTGACACCCCGTACCGACGATCCGGAAGAGACCGTACGTAAACGTCTGGTGGAATACCATCAGATGACTGCACCGCTGa         <  8:930988/140‑1 (MQ=255)
                                                                                                                          tttaaCGTTAAATTCAATCCGGCGAAAGTTTAAGGGAAAGCCGACGTTACCGGTGAAGAACTGACTAACCCTAAATACCATCATGAAGAGACAGTTCGTAAAAGTCTGGTTGTATACAAACAGATTACTGCACCCCTg          >  6:635690/5‑138 (MQ=255)
                                                                                                                          tATCACGTTAAATTCAATCCGCCGAAATTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCCTAAAGACGATCAGGAAGAGACCGTACGTAAAAGTCTGGTTGAATAACATAAGATTACTGCACCGcgg          >  6:496062/1‑136 (MQ=255)
                                                                                                                          tATCACGTTAAATTAAATCCGCCGAAAGTAGAAGGCAAAGACGACCGTACCGGGGAAGCACTGACAACCCGTAAAGCCCATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTg          >  6:335695/1‑138 (MQ=255)
                                                                                                                           aTCACGTTAAATTCAATCCCCCGAATTTACAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGa         <  1:831964/138‑1 (MQ=255)
                                                                                                                              aCGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCg      <  2:19636/138‑1 (MQ=255)
                                                                                                                                tttAAATTCAATCCGCCCAACGTCTAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGc    <  6:831267/137‑1 (MQ=255)
                                                                                                                                gtaaaaTACAACCCCCCGAAAGTAGAAGGAAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGc    <  1:1023736/135‑1 (MQ=255)
                                                                                                                                gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGcta  >  7:28761/1‑140 (MQ=255)
                                                                                                                                    aaTTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGaa                                                             <  3:1001629/77‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
AGGCAGACGCGATGAAAGAAGCGGGCATCAATGTTGATTACGTTCTGGAATTCGACGTACCGGACGAACTGATTGTTGACCGTATCGTAGGCCGCCGCGTTCACGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGACGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACTGCACCGCTGATCGGCTA  >  NZ_CP022686/4056826‑4057093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A