Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F2 I0 R1 74 6379.7 5468672 98.4% 5381173 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,248 T→G 9.1% Q180H (CAA→CAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2480TG 9.1% 210.3 / 3.9 122Q180H (CAA→CAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (29/78);  new base G (9/5);  total (39/83)
Fisher's exact test for biased strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCG  >  NZ_CP022686/1674109‑1674386
                                                                                                                                             |                                                                                                                                          
ggcgCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGcggtcggt                                                                                                                                            <  1:9232/140‑1 (MQ=255)
  cgcTGCCGGGTTGCATTTGCTCTACAAGTGCATGACGGCGACGCTGAAACTCTAGACTGTATTTATCACTCTTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTGT‑TT‑TTGTACTTCTGGCGCGGTCTGTTg                                                                                                                                          <  5:419779/140‑1 (MQ=255)
     tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                       <  5:79248/140‑1 (MQ=255)
     tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                       <  6:37724/140‑1 (MQ=255)
     tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                       <  4:429892/140‑1 (MQ=255)
     tGCCGGGGTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                         <  8:424349/138‑1 (MQ=255)
     ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGTGGCgg                                                                                                                                       <  6:145523/139‑1 (MQ=255)
      gCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGa                                                                                                                                      <  5:100343/140‑1 (MQ=255)
       ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                     <  5:512861/140‑1 (MQ=255)
       ccGGGGTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                     <  1:345880/140‑1 (MQ=255)
        cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATg                                                                                                                                    <  2:367765/140‑1 (MQ=255)
        cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATg                                                                                                                                    <  3:307903/140‑1 (MQ=255)
               cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAg                                                                                                                             <  2:120636/140‑1 (MQ=255)
               cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTaa                                                                                                                               <  8:397173/138‑1 (MQ=255)
               cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTTGCGCGGTCGGTTTCGGATGAGTaaaa                                                                                                                             >  5:360355/1‑139 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  3:478703/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  5:641224/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  4:424193/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  4:212939/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  3:81903/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  6:581319/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  3:441918/140‑1 (MQ=255)
                   tGCTCTACCAGGGCCTGAAGTCGACGCTGAAACTCTTGCCGGTATATATCACTCATAACACTCTCCTTACATATTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTCCTTCTGGCGCGTTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  5:89572/140‑1 (MQ=255)
                    gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTTCTTGGGGCGCGGTCGGTTGGGGATGAGTAAATgtgtg                                                                                                                        >  4:202165/1‑139 (MQ=255)
                     gtctACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                       <  1:398246/139‑1 (MQ=255)
                     ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATGACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                       <  5:44724/140‑1 (MQ=255)
                     ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  7:562090/138‑1 (MQ=255)
                     ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                         <  7:104973/138‑1 (MQ=255)
                     ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGA‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                       <  2:267492/140‑1 (MQ=255)
                         aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGTGGCGGGCCGGTGAGGAATAGTTAAGGTGTCGTGGc                                                                                                                   >  6:538662/1‑140 (MQ=255)
                           cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAt                                                                                                                 <  6:400643/140‑1 (MQ=255)
                              ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCCTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc                                                                                                              <  5:434823/140‑1 (MQ=255)
                               gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑GT‑TTGTACTTATGGCGCGGGAGGTTGCGGATGAGTTTAGGTGTCGTGGGATAACa                                                                                                             >  4:640288/1‑140 (MQ=255)
                               gCCAGACGGCGACGCTGAAAATCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTATTTAGTGTATAGTCGGTGTT‑TTGT‑CATGTGGCGCGGGCGGTTGGGGATGAGGAAAGGGGTCGGGGCAGAAcc                                                                                                             >  4:49774/1‑139 (MQ=255)
                                ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                                            <  1:602651/140‑1 (MQ=255)
                                ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                                            <  2:347713/140‑1 (MQ=255)
                                  tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTTTAATTGTTGGGCGGTCGGTTGCGGATTAGTAAAGGTGTGGTGGGATAACAgcg                                                                                                          >  2:201211/1‑140 (MQ=255)
                                  tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGAGGGGGGGGGTGCGGTTGAGTTAATGTGGAGTGGGAAAACAccg                                                                                                          >  1:65747/1‑140 (MQ=255)
                                    aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TTGTTGT‑GTTGTGGCGCGGGGGGGTGCGGATGGGGAAAGGGGGCGGGGCATAACAGcgcg                                                                                                        >  3:349264/1‑140 (MQ=255)
                                    aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTGTTCTGGGGCGGTCGGTTGGGGATGAGTTAAGGTGTGGTGGCATAAAAGcgcg                                                                                                        >  6:331064/1‑140 (MQ=255)
                                    aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTCTTCGGGCGGGGTGGGATGCCGATGAGTAAAAGTGGCGTGGCAAAACAccgag                                                                                                        >  4:405479/1‑138 (MQ=255)
                                    aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTTCTTATTGCGCGGTCGGTTGAGGATGAGTTAAGGTGTAGTGGGCTAACAGcgcg                                                                                                        >  1:82137/1‑140 (MQ=255)
                                    aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACGTATGGCGCGGTCGGTTGCGGATGAGTAAAGGGTTCGTGGGATAACAGcgcg                                                                                                        >  2:509599/1‑140 (MQ=255)
                                    aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTCTGGCGCGGTCGGGTGCGGATGAGGAAAGGTGTGGTGGCATATCAGagcg                                                                                                        >  2:278064/1‑140 (MQ=255)
                                     cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                       <  2:405227/140‑1 (MQ=255)
                                     cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGATTTTAGTGAAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGGTGCGGAGGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                       <  1:220624/140‑1 (MQ=255)
                                      ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                        >  8:4800/1‑138 (MQ=255)
                                      ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGc                                                                                                      <  4:11769/140‑1 (MQ=255)
                                         gACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACTTATGGCGCGGTCGGTTGCGGATGTGTTAAGGTGTGGTGGCATAACAGCGCGGCAac                                                                                                   >  2:375347/1‑140 (MQ=255)
                                         gACGCTGAAACTCTTGCCGGGATAGATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGGCGGGTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCa                                                                                                     <  7:641285/138‑1 (MQ=255)
                                          aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTATAGTAGGG‑TT‑TTGTTCTTCTGGCGCGGTCGGGTGCGGATTATTAAAGGTTTCGTGGGCTAACAGCGCGGCAaca                                                                                                  >  2:503392/1‑140 (MQ=255)
                                          aCGCTGAAACTCTAGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                  <  1:132547/140‑1 (MQ=255)
                                               gAAACTATTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGA‑TT‑TTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGAc                                                                                             <  6:652223/140‑1 (MQ=255)
                                                        gCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTTCTTCTGGCGCGGGCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACACCGCGGCCACACCGACGTAATCgat                                                                                    >  3:341913/1‑140 (MQ=255)
                                                         ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCga                                                                                     <  7:4800/138‑1 (MQ=255)
                                                           gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAGAGCGCGGCAACACGGACGTACTCgatgat                                                                                 <  3:380772/140‑1 (MQ=255)
                                                           gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                 <  6:99163/140‑1 (MQ=255)
                                                                      cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGGACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                                                      <  2:411072/140‑1 (MQ=255)
                                                                       tCATATCACTCTCCTTAGATTTTTTGTTTTTAGTGTAGAGTCGGT‑TG‑TTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                     <  3:235856/140‑1 (MQ=255)
                                                                       tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                     <  6:409208/140‑1 (MQ=255)
                                                                       tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                     <  1:509599/140‑1 (MQ=255)
                                                                       tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTATGTCGCGGGCGGTTGGGGGTGTGGTAAGGTGGCGTGGCATAACAGCGCGGGACCACGGCCGTACTGGATGAGCTCTTCAAGCgg                                                                     >  2:230052/1‑139 (MQ=255)
                                                                                tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGGCGTGGCATAACAGCGCGGCAACCCGGCCGTACTCGATGATCTATTCAAGCGACAATTCAAg                                                            >  4:504725/1‑140 (MQ=255)
                                                                                 ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTACTTATTGCGCGGGCGGTTGAGGATGAGTAAATGGGTCGTGGCATAACAGCGCGGGCAAACCGAAGTACTCGATGATATCTTAAATCGACATTTaaatc                                                           >  4:160080/1‑138 (MQ=255)
                                                                                 ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTTTAATTATAGCGCGGTGGGTTGCTGAAGATAAAATGTGTGGTGGCCTAACAGCGCGGCCACAACGACCTTAACGATGATCTAATGAAGCCACATTTaaatc                                                           >  6:93399/1‑138 (MQ=255)
                                                                                 ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                           <  2:151490/140‑1 (MQ=255)
                                                                                 ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAATTATGGCGCGATACGTTGAGGATGAGTAAAGGTGTGGTGGCATAACAGCGCGGCAACACCGACGGGCTCGCTGATCTCTTCAAGAGACATTTCAAGc                                                           >  4:517341/1‑140 (MQ=255)
                                                                                  tacTTACATTTATTGTTTTTAGTGTAGAGTCTGT‑GT‑ATGTACTTCAGGCGCGGTCGGTTGCGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTAAAGCGACATTTCAAGct                                                          <  3:681374/138‑1 (MQ=255)
                                                                                   ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTAGTTCTGGCGGGGTCGGTTGCGGATGAGTAAATGTGTAGTGGGATAACAGCGCGGCAACACGGACGTACTCGATGATATCTTAAAGCGCCATTTAAAgg                                                           >  8:306525/1‑137 (MQ=255)
                                                                                   ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGGGCGGTGGGTTGCGGATTAGTTAAGGTGTCGTGGCATAAACGCGCGGCAACACGGACGTACTAGAAGATCTATTAAAGCGACAATTaaaccaa                                                         >  2:687518/1‑136 (MQ=255)
                                                                                    cTTACATTTTTTGTTTTTAGTGTAGAGTCGGG‑TT‑TTGTACATATGGCGCCGTCGGTTGCGGAAGAGTAAAGGAGTCGGGGGATAACACCCCGGCAACACCGACGGACTAGAACATCAAATGAAGCGACATTTAAAggg                                                          >  8:612662/1‑136 (MQ=255)
                                                                                       aCATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                     <  3:413802/140‑1 (MQ=255)
                                                                                                    ttAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                        <  1:674588/140‑1 (MQ=255)
                                                                                                         gtAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                     <  7:228487/138‑1 (MQ=255)
                                                                                                            gagTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa                                <  1:475376/140‑1 (MQ=255)
                                                                                                             agTCGGG‑TT‑TTGGACATATGGGGCGGTGGGTTGCGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAAAAAGGCCGGACTCGATGAACACTTCAAGCGCAATTTAAAGCAGCTCCGGATCTACGTCATAGTGGTAAc                               >  4:665085/1‑140 (MQ=255)
                                                                                                                 ggT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                           <  3:282254/140‑1 (MQ=255)
                                                                                                                 ggT‑TT‑TTGTACTTCTCGAGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAGATCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                           <  3:489596/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCTGTCGGTTGCGGATGATTAAAGGTGTCGGTGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAAATTTCAATCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  1:325627/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  3:685640/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  5:143839/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  2:545672/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAGCTTCGTCTTCGTCGTAACCCAACTgcg                      <  2:499468/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  5:4246/140‑1 (MQ=255)
                                                                                                                        ttGTACTTCTGGCGCGGGCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  5:352212/140‑1 (MQ=255)
                                                                                                                         tGTACTTATGGCGCGTTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGAAGTAAACGAAGATCTCTTAAAGCGACAATTCAAGCTCTTCCAGATCTTCGACTTCGTCGTAACCACACAgcgc                     >  3:182399/1‑140 (MQ=255)
                                                                                                                          gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  7:491211/138‑1 (MQ=255)
                                                                                                                          gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                    <  1:438015/140‑1 (MQ=255)
                                                                                                                          gTACGTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                    <  2:431565/140‑1 (MQ=255)
                                                                                                                            aCTTCTGGCGCGGTCGGTTGCGGAGGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGAt                  <  4:631173/140‑1 (MQ=255)
                                                                                                                                 tGGCGAGGTCGGTAGCGCATGAGTAAAGGTGTCGTGGCATAACCGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTCCTCGATGtt               >  7:178019/1‑138 (MQ=255)
                                                                                                                                  ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAATCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg            <  4:535136/140‑1 (MQ=255)
                                                                                                                                  ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTa              <  7:569013/138‑1 (MQ=255)
                                                                                                                                  ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTa              <  7:572099/138‑1 (MQ=255)
                                                                                                                                  ggCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg            <  3:154773/140‑1 (MQ=255)
                                                                                                                                  ggCGCGGGCGGTTGCGGATGAGTAAAGGGGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg            <  5:409374/140‑1 (MQ=255)
                                                                                                                                   gcgcGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGc           >  4:329488/1‑140 (MQ=255)
                                                                                                                                     gcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGAATATATCTTCAAGCGACATTTCAATCTCTTCCTGATCTTCGTATTCGTCGTAACCCAACTGCTCGATGTTACga           >  8:536168/1‑137 (MQ=255)
                                                                                                                                      cggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa        <  2:631478/140‑1 (MQ=255)
                                                                                                                                            gtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtcgtcg  <  6:601790/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                          
GGCGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGT‑TT‑TTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCG  >  NZ_CP022686/1674109‑1674386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A