Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F36 I0 R1 275 10897.2 10515506 98.4% 10347257 135.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,248 T→G 12.8% Q180H (CAA→CAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2480TG12.8% 388.0 / 14.1 221Q180H (CAA→CAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (49/138);  new base G (29/5);  total (78/143)
Fisher's exact test for biased strand distribution p-value = 8.70e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCG  >  NZ_CP022686/1674111‑1674386
                                                                                                                                           |                                                                                                                                           
ctcTGCCGGTATGCATTTGCTCTACCAGGGACTGACTGCGACGATGAAACTCTTGCAGGGATATATAACTCATAACAATCTCCATCCATTTTTTTTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTg                                                                                                                                           <  8:287751/138‑1 (MQ=255)
cgcTGCCGGGTTTCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGcggtcggt                                                                                                                                             <  2:1207693/138‑1 (MQ=255)
  cTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTGACTTATGG‑CGCGGGCGGTTGCg                                                                                                                                         >  8:209904/1‑140 (MQ=255)
   tggcGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGTGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCgg                                                                                                                                        <  7:1001974/137‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGc                                                                                                                                          <  4:1271998/138‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGc                                                                                                                                          <  1:736902/138‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCgg                                                                                                                                        <  7:521041/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTATTGTACTTCTGG‑CGCGGTCGGTTGCgg                                                                                                                                        <  7:863184/140‑1 (MQ=255)
     ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCgg                                                                                                                                        <  1:790973/138‑1 (MQ=255)
     ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCgg                                                                                                                                        <  1:847730/138‑1 (MQ=255)
     ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGAt                                                                                                                                      <  6:347367/140‑1 (MQ=255)
      cGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATg                                                                                                                                     <  7:23050/140‑1 (MQ=255)
          ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAg                                                                                                                                   <  3:889048/138‑1 (MQ=255)
             cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTaa                                                                                                                                <  1:504994/138‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACTGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCTGATGAGTAAAGgtgt                                                                                                                          <  8:850088/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  5:214906/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  6:1276651/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                            <  3:165308/138‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                            <  4:727182/138‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGgt                                                                                                                            <  1:575670/138‑1 (MQ=255)
                 gtcgcaaCCAGGGCCGGACGTCTACGCTTAAACACTTGCCGGTTTATCTCCCTCATAACACTCTCCTTACATTTTTTGTTTTTATTGTAGAGTC‑TGTTTTTGTACTTCTGG‑CGCGGTCGGTGTCGGATGAGTAAAGgtgt                                                                                                                          <  6:65487/134‑1 (MQ=255)
                  gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTCTTATGG‑GGCGGGGGGTTGGGGGTTTGTTAAGgtgtg                                                                                                                         >  5:371967/1‑139 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGGCGGTTGCGGATGTGTAAAGgtgt                                                                                                                          <  1:19094/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  2:154024/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                        <  7:754952/140‑1 (MQ=255)
                    tctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTCATATGG‑CGCGGTCGGGTGCGGATGAGTAAAGgggtg                                                                                                                         >  4:1072238/1‑137 (MQ=255)
                         cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTCTGT‑CGCGGGCGGGTTCGGATTAGTTAAGTTGTCGTggg                                                                                                                    >  2:346273/1‑137 (MQ=255)
                          aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGGTGCGGATTAGTAAAGGTGTCGTGGcc                                                                                                                   >  2:1030465/1‑137 (MQ=255)
                             gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTGATGTTG‑CGCGGGCGGGTGGGGGTGAGGTAAGGGGGCGGGGGCTAACa                                                                                                              >  6:261674/1‑140 (MQ=255)
                                  aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑TGTTTTTTGACTTCTGG‑CGCGGTGGGTTGGGGATGAGTAAAGGTGTCGGGGCATTACacaggg                                                                                                         >  8:307854/1‑135 (MQ=255)
                                  aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTCTTCGGG‑CGCGGGCGGTTGCGGATGAGTTAAGGTGGCGTGGCATAACcgcg                                                                                                           >  2:931042/1‑138 (MQ=255)
                                  aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTAATATGG‑CGCGGTCGGGTGCGGTTGAGTAAAATTGTGGTGGGATAACAgcc                                                                                                           >  1:1010785/1‑137 (MQ=255)
                                    ggCGACGCTGAAACTCTTGCCTGGATATATCACTCATAACAATCTCCTTACATTTTTTTTTTTTAGTGTAGCGTC‑GGGTTTTTTGCTTGTGG‑AGCGGTGGGTGGCAGTGGAGTTAATGTGTCGTGGGGAAAGAGcgcg                                                                                                         >  4:29953/1‑138 (MQ=255)
                                    ggCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑GGCGGGCGGGTGGGGATGAGTTAAGGTGTCGTGGCATAACAGcgcg                                                                                                         >  3:1303014/1‑138 (MQ=255)
                                        ccGCTGCAACTCTAGCCGGGAACTATCACTCATAACACTCTCCTTACATTTTATGTTTTTAGTTTAGAGTC‑GTTGTTTGTACTTCTGG‑CGCGGGCGGGTGCGGATGAGTAAAGGTGTCGTGGCATTACAGCGCGGC‑Aaca                                                                                                  <  8:626009/139‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACCGCGCGGC‑aa                                                                                                    <  4:866008/138‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑aa                                                                                                    <  3:617280/138‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑Aaca                                                                                                  <  8:360370/140‑1 (MQ=255)
                                         cGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTAATTCTTG‑CGCGGTCGGTTGCGGGTGTGTTAAGTTTTCGTGGCATAACCGCGCGGC‑Aac                                                                                                   >  2:939810/1‑138 (MQ=255)
                                          gCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTATTGTGG‑CGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGGATAAAAGCGCGGC‑Cacc                                                                                                  >  3:211907/1‑137 (MQ=255)
                                           cTGAAACTCTGGGCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGATTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACgg                                                                                               <  8:46323/140‑1 (MQ=255)
                                            tGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGa                                                                                              <  7:1135860/140‑1 (MQ=255)
                                            tGAAACTCTTGCCGGGATATATCAATCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACg                                                                                                <  2:1270138/138‑1 (MQ=255)
                                              aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACg                                                                                            <  8:278526/140‑1 (MQ=255)
                                              aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTGTTAGTGTAGAGTC‑GGTGTTTGTACTTCTGG‑CGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGa                                                                                              <  4:635849/138‑1 (MQ=255)
                                               aaCTCTTGCCGGGATTTCTCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGAc                                                                                             <  4:424525/138‑1 (MQ=255)
                                                aCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACGTCAGG‑CGCGGTCGGTTGCGGAGGAGGTAAGGTGTGGTGGAGTAACAGCGCGGG‑CACACGGACg                                                                                            >  4:190259/1‑138 (MQ=255)
                                                 ctctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGt                                                                                           <  4:1057049/138‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCgatgat                                                                                 <  8:1026460/140‑1 (MQ=255)
                                                                        tAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTCTTCTGG‑CGGGGGCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑CACCCCGACGGACGCGCTGTTCTCGTCAAACgcc                                                                    >  3:549482/1‑136 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTATGG‑GGGGGGCGGGTGGGGTTGAGTTAATTTGTAGGGGGAAAAAAGCGCGGC‑AAAACGGCCGTAAACGTTGATAGCTTAAAGCGACAtt                                                                 >  8:1182566/1‑140 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTATGG‑CGCGGTCGGTTGTGGATGAGTATAGGTTTCGGGGCATAACCCCGCGGG‑CAAACGGCCGTAATCGAGTAACTGTTCAAGCGCCa                                                                   >  4:56296/1‑138 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTATTG‑CGCGGTGGGTTGCGGATTAGTAAAGGTGTCGTGGGATAAAATCGCGGC‑AACACGGACGTACTCAATGATATATTCAATCGACa                                                                   >  3:492678/1‑138 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTATGG‑CGCGGTCGGTTTGGGATTAGTAAAGGTGTAGTGGCATAACAACGCGGA‑CAAACGGACGGACTCGATGTTCTCTTCAAGCGACa                                                                   >  2:824258/1‑138 (MQ=255)
                                                                           cacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTAATTATGG‑CGCGGTAGGTTGCGGATGAGTAAAGGTGTAGTGGGATAAAACCGCCGC‑AAAACCGACGTACTCGATGATAAATTGAAGCGACAtt                                                                 >  2:1301383/1‑138 (MQ=255)
                                                                            acTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑TGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                                              <  6:341333/140‑1 (MQ=255)
                                                                              tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                            <  6:783883/140‑1 (MQ=255)
                                                                              tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGACGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCa                                                              <  2:3215/138‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTTATTATGG‑CGGGGTCGGGTGGGTAAGAGTTAAGGTGTCGTGGGATACCAGCGCGGC‑AACACGGGCGTACTAGATTCTATCTTCAAGCGACATTTCAAGc                                                           >  7:180087/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                           <  6:428292/140‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCGGG‑CGCGGTCGGGTGCGGCTGTGGAAAGGTGTCGTTGCATTACAGCCCGGC‑ACCACGGAACTACTCGAAGATCGGGTCAAGCGACAATTAAAGc                                                           >  8:506554/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTCTTCTGGACGC‑GTCGGTTGCCGATGAGTTAAAGAGTGGAGGCATAAAAGCGCGGC‑AAAACGGACGTACTCGAAGTTCTCTTCAAGCGACATTTaaaac                                                           >  6:116616/1‑138 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTCTTCTGG‑CGCGGGAGGGTTCGGATGAGTAAATGTTTGGTGGAATAAAAGAGCGGC‑AACACGGCCGGAAAAGAAGATATCATCAAGCCACAtttaacac                                                           >  5:70894/1‑135 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGG‑AGCGGTAGGGTGCGGATTAGTTAATTTGTCGGGGCATTAAAGAGCGGCAAACAC‑GACGTCCTAGATGATATCTTAAATCCACATTTaaatc                                                           >  6:833358/1‑138 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGGACTTCTGG‑CGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCCTAACAGCCCGGC‑CACACCGACGTACGAGATGATCTCTTCAATCGCCATTTCAAgg                                                           >  8:412970/1‑139 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTATA‑TAGTGG‑CGAGGGCGGGTGAGGGAGAGTAAATGTGTCGTGGCTTAACAGCGCAGC‑AACACGGACGTACTCGAAGATATCATAAACCGACAATTaaaag                                                           >  4:1160877/1‑136 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTATTCTTT‑CGCGGGAGGGTGCCGATGTGTAAAAGTGTCGTGGGAAAACAGCGCGGC‑ACAACCGCCCTACACGATGATCTCATCAACCGACATTTaaatc                                                           >  2:386428/1‑136 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTATTATGG‑CGCGGGCGGGTGGGGATGAGTAAAGGTGTATTGGCATAACAGCGCGGC‑ACCACGCCCGGCCTCGATGAACTCTTAAAGCGGCAATTCAAGc                                                           >  4:846549/1‑138 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGATTTTGTACTTATGG‑CGCGGTCCGGTGCGGATTAGTAAAGGTGTAGTGGGATAACAGCGCGGC‑CACACGGTCGTACAGCATGATCTAATGAAGCGGCATTTAAAGc                                                           >  4:395378/1‑138 (MQ=255)
                                                                                  cTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTACGTATGG‑CGCGGTCGGGTGCGGTTGTGTTAAGGTGTCGGGGCATAACACCGCGGC‑ACCACGGACGTACTCGATGATCTGTTAAAGCGACATTTAAAGctgt                                                        >  6:1117585/1‑138 (MQ=255)
                                                                                      cATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTc                                                      <  2:258480/138‑1 (MQ=255)
                                                                                          ttttGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTTAAGGTGTCGTGGCATAACAGCGCCGC‑ACCACGGACGTACACGATGATCTCTTCAAGCGACAATTAAAGCTCTTCCTGa                                                  >  4:611964/1‑138 (MQ=255)
                                                                                           tttGTTTTTAGTGTAGAGTC‑GGGTTTTTTTCTTCTGG‑GGCGGTGGGGTGCGGATGTGTAAAGGGGTAGTGGCAAAAACGCGGAGC‑CACACGGCCCTAAACGATGAGAGATTCAAGCGACAATTAAAGCTCATCCTGAtat                                               >  8:548965/1‑138 (MQ=255)
                                                                                                 tttAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                                           <  3:1318734/138‑1 (MQ=255)
                                                                                                  ttAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCTGTTGAGGATGAGTAAAGCTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                        <  8:594101/140‑1 (MQ=255)
                                                                                                          gagTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTcgtcgt                                  <  3:658293/138‑1 (MQ=255)
                                                                                                          gagTC‑GGTGTTTGATCTTCTGG‑AGCGGTCGGTTGCGCATGAGGAAAGGGGTCGTGGCAATACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTACTGATCTTCGTCTTCGTCGTaa                                <  7:802164/140‑1 (MQ=255)
                                                                                                                gggttttttAATTATGG‑GGCGGTAGGTTGCGGCTTATTAAATGTGTCGTGGCCTAACAGCGCGGC‑AACACGGGCGTAAAAGAGGATAACTTAAATCGGCAATTCAAGGAAATCCTGAGAATAGTCTTCGTACGAACCCaa                           >  8:796669/4‑140 (MQ=255)
                                                                                                                gggttttGTACTTCTGG‑CGCGGGCGGGTGGGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTTCTCGATGATCGCTTGAAGCGACAATTCAAGCTCTTGCTGATATTAGGATTAGTCGTAAccc                             >  4:144963/4‑138 (MQ=255)
                                                                                                                ggTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCaa                           <  6:371967/140‑1 (MQ=255)
                                                                                                                ggTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCCCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAAccc                             <  1:1171385/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  6:1018433/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                      <  8:892425/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  4:656531/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  3:811549/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  2:630369/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  1:436330/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  2:1008587/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  1:670236/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAAATgcg                      <  5:4247/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGAGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  1:313211/138‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGG‑CGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                        <  1:1234400/138‑1 (MQ=255)
                                                                                                                       ctACTTCTGG‑CGCGGTCTGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCTCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGTCATTTCAAGCTCTTCCTGATCTTCGGCTTCGTCGTAACCCAACTgcg                      <  2:457956/137‑1 (MQ=255)
                                                                                                                        tACTTCTGG‑AGCGGTCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGa                   >  6:302667/1‑140 (MQ=255)
                                                                                                                          cTTCTGG‑CGCGGTCGGTTGCGGATGATTAAAGGTGGCGTGTCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATg                 <  5:97163/140‑1 (MQ=255)
                                                                                                                            tCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt               >  5:489190/1‑140 (MQ=255)
                                                                                                                              tGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt               >  2:534756/1‑138 (MQ=255)
                                                                                                                              tGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt               >  1:384165/1‑138 (MQ=255)
                                                                                                                                        cggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc      >  1:138446/1‑138 (MQ=255)
                                                                                                                                          gtTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGC‑AACTCGGACGTACTCGATGATCTCTGCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtcgtcg  <  5:1018097/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGG‑CGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGC‑AACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCG  >  NZ_CP022686/1674111‑1674386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A