Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F35 I0 R1 174 9808.2 8488800 98.4% 8352979 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,248 T→G 13.7% Q180H (CAA→CAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2480TG13.7% 309.8 / 11.9 179Q180H (CAA→CAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (35/115);  new base G (24/5);  total (59/120)
Fisher's exact test for biased strand distribution p-value = 1.98e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCGA  >  NZ_CP022686/1674111‑1674387
                                                                                                                                          |                                                                                                                                           
cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTTCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTATGGGGCGGTGGGTTg                                                                                                                                           >  5:910728/1‑140 (MQ=255)
cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GGTTTGTTCTTCGGGGGCGGTCGGGTg                                                                                                                                           >  3:453922/1‑140 (MQ=255)
cgcTGACGGGTTGCATTTGCTCTACCCGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACGCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCGTCTGTCGCGGTCGGGTg                                                                                                                                           >  4:831951/1‑140 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                        <  6:608260/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                        <  5:17361/140‑1 (MQ=255)
     ctgggTTGCATTTTCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTT‑TTTTTGTACTTCTGGCGCGGTCGGTTggtgat                                                                                                                                      <  8:383372/138‑6 (MQ=255)
     ccGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                      <  6:691144/140‑1 (MQ=255)
     ccGGGTTGCATTTGATCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACTCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGac                                                                                                                                      <  6:986875/140‑2 (MQ=255)
        ggTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAg                                                                                                                                   <  4:1024975/140‑1 (MQ=255)
          ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACA‑‑‑‑‑‑‑TTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  6:157279/140‑1 (MQ=255)
             cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTTCTTGTGGCGCGGGGGGGTGGGGGTGAGTTAAg                                                                                                                              >  8:458919/1‑140 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  4:296433/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  8:291566/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGATGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  5:967107/140‑1 (MQ=255)
                  gCTCTACCAGGGCCTGACGGCGACGCTGACACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGTCTG‑GTTTATTACTTCTGGAGCGGGCGGTATCGGATGATTaaaagaggg                                                                                                                         >  4:804160/1‑134 (MQ=255)
                  gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAAGTCTGGCGCGGTCGGTTTGGGAGGAGTAAATgtgtg                                                                                                                         >  5:681271/1‑139 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  2:713632/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGgtgt                                                                                                                          <  2:415352/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                        <  3:940481/140‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATAGCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                        <  6:537055/140‑1 (MQ=255)
                      tACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTGCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGAGTAAAGGTGTCGt                                                                                                                       <  2:239209/138‑1 (MQ=255)
                      aaCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCTCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                                     <  8:943306/139‑1 (MQ=255)
                       aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCTTCTGGCGCGGTCGGTTGCGGGTGAGTTAAGGTGTCGTggg                                                                                                                    >  3:1015980/1‑139 (MQ=255)
                         cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCAt                                                                                                                  <  3:921239/140‑1 (MQ=255)
                         cAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTGCGTCTGGCGCGGGCGGTTGGGGGTGAGTAAAGGTGGCggtgg                                                                                                                    >  2:736940/1‑134 (MQ=255)
                          aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTCTGGCGCGGTCGGTTGCGGATGAGTTAATGTGTCGTGGcg                                                                                                                   >  1:625104/1‑137 (MQ=255)
                            ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTTCTTATGGCGCGGGAGGTGTGGGTTGTGTATAAGTGTCGTGTCaaaaa                                                                                                               >  7:612617/1‑139 (MQ=255)
                                tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                           <  4:735033/140‑1 (MQ=255)
                                tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGCGGGGGGGGATGAGTAAAGGTGTCGTGGCATAACAtcg                                                                                                           >  5:939754/1‑140 (MQ=255)
                                tGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTGATGGCGCGGTCGGGGGCGGATGAGTTATGGTGTGGGGGGATAACAggg                                                                                                           >  6:579478/1‑138 (MQ=255)
                                  aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTAATTATGGCGAGGTGGGGTGGTGATGAGTATAGGTGTCGTGGGCTATCAGcgcg                                                                                                         >  8:713490/1‑140 (MQ=255)
                                  aCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAAACCTCTCCTTACATTTTTTGTTGTTAGTGTAGAGTCGG‑TTTTTTTTTTTATGGCGCGGTGGGGTGCGGATGAGTTAATTGGTGGTGGCTTTACAGcgcg                                                                                                         >  6:859617/1‑140 (MQ=255)
                                   cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGGGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                        <  8:553385/140‑1 (MQ=255)
                                      cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGGGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                     >  8:585134/1‑140 (MQ=255)
                                      cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                     <  4:35215/140‑1 (MQ=255)
                                        ccGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGACATAACAGCGCGGCAaca                                                                                                   <  3:124747/139‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATGACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGAGG‑TTTTTGTACTGCTGGCGCGGTCGGTTGCGGTTGAGTAAAGGTGTCGTCGCATAACAGCGCGGCAaca                                                                                                   <  3:1069608/140‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                     <  2:908595/138‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                   <  7:905274/140‑1 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTACTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCaa                                                                                                     <  2:364478/138‑1 (MQ=255)
                                        aCGCTGAAACTCTTGACGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                   <  7:269747/140‑1 (MQ=255)
                                            tGAAACTCTTGCCGCGATATATCACTCATAACACCCACCTGACATTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTGTAATGTTGGC‑CGGGGGGGTGACGGTGGCTAAACGTGTCGTGGCAGTACCGCGCGGCcacagcgc                                                                                               >  8:490278/1‑136 (MQ=255)
                                              aaaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGa                                                                                               <  1:584889/138‑1 (MQ=255)
                                               aaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGt                                                                                            <  3:992253/140‑1 (MQ=255)
                                               aaCTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCTGTCGGTTGCGGATGTGTAAATGTGTCGTGGGATAACAGCGAGGCAACACGGACGt                                                                                            <  5:347079/140‑1 (MQ=255)
                                                aCTCTTGCCGGGATATATCACTCATAACACTCTCCTTCCATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTa                                                                                           <  5:801560/140‑1 (MQ=255)
                                                  tctTGCCGGGATATATCACTCATAACACTATCCTTACATTATTTGTTTTTAGTGTAGAGGCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTCGCATAACAGCGCGGCAACACGGACGTa                                                                                           <  2:124880/138‑1 (MQ=255)
                                                    tttccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCTGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACt                                                                                         <  2:608804/135‑1 (MQ=255)
                                                       ccGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCga                                                                                      <  2:1024034/138‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCTG‑TTTTTGTACTTCTGGAGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                  <  3:322859/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                  <  6:1002463/140‑1 (MQ=255)
                                                           gATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGAtct                                                                                <  5:178338/140‑1 (MQ=255)
                                                                    cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTTTAGAGTCGG‑TGTTTGTACATCAGGCGCGGGCGGGTGCGGATTTGTAAAAGGTTCGTGGCATAACAGCGAGGCGACCCCGACCTGAGCGCTGATCTGTTGAAGCg                                                                       >  8:881404/1‑140 (MQ=255)
                                                                    cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCg                                                                       >  7:833653/1‑140 (MQ=255)
                                                                     tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTGTGGGGCGGTCGGTTGCGGAAGAGGTAATGTGTGGGGGGATAACAGCGCGTCCACAAGGTCGGACTCGATGATCTCTTCAAGCgg                                                                      >  3:685532/1‑139 (MQ=255)
                                                                     acATAACACTCTACTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGAGGATGAGTAAAGGTGTCGTGGCATTACAGCGCGGCAACTCGGACGTACTCGATGATCTCTTCAAGCGa                                                                      <  3:269509/139‑1 (MQ=255)
                                                                          acacTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACAttt                                                                 <  7:642703/140‑1 (MQ=255)
                                                                             ctctCCTAACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                                              <  8:508683/140‑1 (MQ=255)
                                                                              tctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTGTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                             <  5:1011956/140‑1 (MQ=255)
                                                                               ctcGTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGTCGGTTGCGTTTGATTAATGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                            <  8:566894/140‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTTCTTCTGGCGCGGTCGGGTGGGGATGAGTAAAGGTGTCGTGGCATAAAAGCGCGGCCAACCGGTCGTTGGCGATGTTCTAATCAAGCGACAGTTAAAGc                                                            >  6:138549/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGTCGCGGTCGGTTGCGGATGAGTAAAGGTTGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                                              <  1:19409/138‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                            <  8:734749/140‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGGCGGTTGAGGATTTGTTAATGTTTCGTGGCATAACAACGCGGGAACAATGCCGTACTAGATGATCTCTTCAAGAGACATTTCaatg                                                            >  6:5088/1‑138 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTTTTATTATGGAGCGGTCGGGTTAGGCTGATGTAAGGTGTGGTGGGATAAAAACGCGGCCACACGGACCTACTCGATGATCTCTTAAAGAGCCAATTTAAGc                                                            >  4:5020/1‑140 (MQ=255)
                                                                               ctcCTTACATATTTTGTTTTTAGTGTAGAGTCGG‑TTTTTTTACTTATGGCGCGGGCGGGTTCGGCTGTGTAAATGTGTGGTGGCATAACAGCCCGGGCACCCGGACGAACTCGATGATCTCCTAAAGCGTCCATTCAAGc                                                            >  6:953738/1‑140 (MQ=255)
                                                                               ctcATTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGc                                                            <  3:218677/140‑1 (MQ=255)
                                                                                tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTATGGGGCGGGCGGGTGGGGATGAGTAAAGGTGTCGTGGCAAAAAAGCGCGGCACAACGGACGTACTCGATGATCTCTTCAAGCCACATTTCaac                                                             >  1:287132/1‑137 (MQ=255)
                                                                                tcCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTAAGGCGCGGGCGGGTGGGGATGAGTTAATGTGTCTGGGCGTAACAGCGGGGGACCACGCGCGTTCTCGATGATCTCTTGAAACTAAATTTAAAGcg                                                           >  8:631690/1‑139 (MQ=255)
                                                                                 ccTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTAATTATTGCGCGGGAGGGTGCGGATTATGAAAGGTGTCTGGGCATAAAAGAGCGGCAACACGGACGTACTAGATTATCTCTTACATCGACATTTAAAGcta                                                          >  4:758847/1‑139 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTCGG‑GTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAATGTGTAGGGGCAATACAGCGCGGCTACCCGGACGGACACGATGATCTAATCAAGCGACAATTCAAGCTCTTcc                                                      >  4:928724/1‑140 (MQ=255)
                                                                                          ttttGTTTTTAGTGTAGAGTCGG‑TTTTTGTTCTTCTGGCGCGGTCGGGTGCGGATGAGTAAAGGTGTCGTGGCATAACCGCGCGGCAACACGGCCGGACACGTAGATCTCTTCAAGGGACCTTTCAAGCTGTTACGGAtg                                                 >  6:430001/1‑139 (MQ=255)
                                                                                                 tttAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                                          <  4:631555/140‑1 (MQ=255)
                                                                                                 tgtAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGTTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGAGCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtct                                          <  8:694050/138‑1 (MQ=255)
                                                                                                  ttAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtctt                                         <  8:1014770/140‑1 (MQ=255)
                                                                                                  ttAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtc                                           <  1:92568/138‑1 (MQ=255)
                                                                                                     gtgtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                        <  1:1073379/138‑1 (MQ=255)
                                                                                                     gggtAGTGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGGTGCGGTTGAGTAAAGGTGTCGTTGCATAACAGCGCGGCAACACGGCCGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                      <  7:691221/138‑1 (MQ=255)
                                                                                                       gtAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                      <  2:977116/138‑1 (MQ=255)
                                                                                                          gagTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTaa                                 <  7:585134/140‑1 (MQ=255)
                                                                                                                  ttttttTACTTAAGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGAGGCATAACAGCGCGGCAACACGGACGTACTGGATGATCTCTTCAAGCGACATTTCAAGCTGTTCCTGCTCTTCCTCTTAGTAGTAACCCAACt                          >  4:465812/1‑140 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  7:324913/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  7:709403/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  6:1016601/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  5:422009/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  4:877988/140‑1 (MQ=255)
                                                                                                                       gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  1:114462/138‑1 (MQ=255)
                                                                                                                       gTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                     <  4:969539/140‑1 (MQ=255)
                                                                                                                       gTACTTCTGGCGCGGGCGGTTGCGGATTTGTAAATGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTATATGATCTCTTCATGCGAGATTTAAAGCTCTTCCAGATATTCGTCATCGTCTTAACCCAACTgctct                     <  4:198749/140‑4 (MQ=255)
                                                                                                                       gTACGTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGAGCCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                     <  8:662032/140‑1 (MQ=255)
                                                                                                                            tCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt                >  3:85275/1‑140 (MQ=255)
                                                                                                                              tGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt                >  1:496663/1‑138 (MQ=255)
                                                                                                                                gcgcGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAAATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTActc            >  8:521319/1‑138 (MQ=255)
                                                                                                                                   cggtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa         <  5:515818/140‑1 (MQ=255)
                                                                                                                                   cgggcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa         <  5:125122/140‑1 (MQ=255)
                                                                                                                                     gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAg                                                                                                             <  3:949798/38‑1 (MQ=255)
                                                                                                                                     gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                     <  3:340634/126‑1 (MQ=255)
                                                                                                                                     gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc       >  7:792038/1‑140 (MQ=255)
                                                                                                                                     gtcggtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc       >  5:258931/1‑140 (MQ=255)
                                                                                                                                          ttGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCGa  <  6:893873/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGG‑TTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCGA  >  NZ_CP022686/1674111‑1674387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A