Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A27 F2 I0 R1 73 6005.1 5484070 98.3% 5390840 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 1,674,248 T→G 14.7% Q180H (CAA→CAC)  ygfB ← UPF0149 family protein YgfB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226861,674,2480TG14.7% 183.1 / 4.3 115Q180H (CAA→CAC) ygfBUPF0149 family protein YgfB
Reads supporting (aligned to +/- strand):  ref base T (23/72);  new base G (17/3);  total (40/75)
Fisher's exact test for biased strand distribution p-value = 5.43e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCGA  >  NZ_CP022686/1674111‑1674387
                                                                                                                                          |                                                                                                                                            
cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTGTGGCGCGGGCGGTTg                                                                                                                                            >  2:587772/1‑140 (MQ=255)
cgcTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTg                                                                                                                                            <  4:564403/140‑1 (MQ=255)
  cTGCCGGGTTGCATTTGCGCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGGTGCg                                                                                                                                          <  4:6075/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACTCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGc                                                                                                                                           <  7:625007/138‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                         <  4:359454/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                         <  3:556983/140‑1 (MQ=255)
   tGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                         <  2:375710/140‑1 (MQ=255)
   ggCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCgg                                                                                                                                         <  2:655680/139‑1 (MQ=255)
    gCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCg                                                                                                                                          <  7:14225/138‑1 (MQ=255)
     ccGGGTTGCATTTGCTCTTCCTGGGCCTGACTGCGACGCTGATACTCTTTCCGGGATTTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GTTTTTTGTACTTCTGGCGCGGTCGGTTGCGGAt                                                                                                                                       <  2:39436/140‑1 (MQ=255)
          ttGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑Ta                                                                                                                                 <  4:259815/140‑1 (MQ=255)
            gCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGAGATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTATTCTGGCGCGGTAGGGTTGGGATGAG‑tt                                                                                                                                 >  7:656044/1‑137 (MQ=255)
             cATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAg                                                                                                                              <  2:692234/140‑1 (MQ=255)
                 tGCTCTAGCAGGGCCTGTCGGGGACGCTGAAACTATTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TTAAGgt                                                                                                                            <  8:608025/138‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGTAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  1:505124/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  5:281181/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  3:549612/140‑1 (MQ=255)
                 tGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  3:163886/140‑1 (MQ=255)
                  gCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTATGGGGCGGTGGGTTTCGGATGAG‑TAAATGTGTc                                                                                                                         >  5:326619/1‑140 (MQ=255)
                   ctctACCAGGGCCTGTCGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCg                                                                                                                        <  1:201855/140‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  7:327745/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  7:88485/138‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCg                                                                                                                        <  2:197441/140‑1 (MQ=255)
                   ctctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCg                                                                                                                        <  1:646028/140‑1 (MQ=255)
                   cgctACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGgtgt                                                                                                                          <  7:136644/136‑1 (MQ=255)
                       aCCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATTTATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTA‑GGGTTTTGTAAGTATGTGGCGGTTGTTTGAGGCTGAG‑TTAATGTGTCGTggg                                                                                                                    >  1:211605/1‑139 (MQ=255)
                         cAGGGCCTGACGGCGACGCTTAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGc                                                                                                                    <  7:377834/138‑1 (MQ=255)
                            ggCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCGTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGGCGGGTGCGGATGGG‑TAAAGGTGGCGTGGCGTaaa                                                                                                               >  5:629521/1‑139 (MQ=255)
                             gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATACCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTGACTTCAGGCGCGGTCGGTTGCGGATGTG‑GAAAGGTGTCGTTGCGTAACa                                                                                                              >  1:407846/1‑140 (MQ=255)
                             gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCGCCTTACATTTTTTGTTTTTAGTGTAGAGGC‑GGGTTTTGTGCTTCGGGCGCGGTAGGTTGGGGGTGAG‑GAAAGGGGTCGGGGCCTAAcc                                                                                                              >  2:146001/1‑139 (MQ=255)
                              ccTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTGTTGTAAGTCTGGCGCGGGCGGTGGGGGATGAG‑TTAAGGTGTCGTGGCATAACAg                                                                                                             >  4:18417/1‑140 (MQ=255)
                               cTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTCGGGTTGTTGT‑CTTAGGGCGCGGTGGGGTGCGGTTTTG‑TAAATGTGGGGTGGCAAAACa                                                                                                              >  7:8966/1‑138 (MQ=255)
                                 gACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAgcgc                                                                                                          <  1:311750/140‑1 (MQ=255)
                                   cGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCATTACATTTTTTGTTTTTAGTGTAGAGGC‑GGTTTTTGTACTTCTTGCGCGGTCGGTTGCGTATGAG‑TAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                        <  5:478373/140‑1 (MQ=255)
                                      cGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTAATTCTGGCGGGGGCGGTGGCGGCTGAG‑TAAAGGTGTGGGGGGGTAAAAGCGCGGccc                                                                                                     >  4:686228/1‑138 (MQ=255)
                                        aCGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                   <  4:254153/140‑1 (MQ=255)
                                          gCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGGCGTGGCATAACAGCGCGGCAACACg                                                                                                 <  4:45002/140‑1 (MQ=255)
                                          gCTGAAACTCTTGCCGGGATATATCACTCATAACACGCTCCATACATTTTTTGTTTTTAGTGTAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACg                                                                                                 <  6:21341/140‑1 (MQ=255)
                                           cTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                                                <  3:289888/140‑1 (MQ=255)
                                             gAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTCGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACgg                                                                                                <  7:342343/138‑1 (MQ=255)
                                                   ctTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTT‑TACTTTTGGCGCGGGCGGTGGCGGCTGAGTTAAAGGTG‑AGTGGCATAACAGCGCCGCAACAAGGACGGACACg                                                                                       >  2:76960/1‑140 (MQ=255)
                                                    ttGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTAAATCTGGCGCGGGCGGGTGCGGATGAG‑TAAAGGGGTGGTGGGATAACAGCGCGGCCACACCGACGTACTCg                                                                                       >  3:432825/1‑140 (MQ=255)
                                                         ttgATATATCAAGAATAAAACTCTCATTACATTTTTTGCTTTTCTTGTAGAGTC‑TGTTTTTGTACTTCTGGAGCGTTCGGTTGCGGATGAT‑TAAAGGTTTCTTGGCATAACAGCGCGGAAACACGGACGTACTCgatgat                                                                                  <  2:156749/138‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTTCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgattat                                                                                  <  4:565965/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                  <  3:479068/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                  <  1:587772/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                  <  1:188479/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatgat                                                                                  <  2:288677/140‑1 (MQ=255)
                                                         gggATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                                    <  8:198060/138‑1 (MQ=255)
                                                         gggAGATATCACTCATAACACTCACCTTACATTTTTTGTGTTTAGTGTAGAGGC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                                    <  8:476487/138‑1 (MQ=255)
                                                              atatCACTCATAACCCTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGGCGCGGTCGGGTGCGGGTGAG‑TAAAGGTGTAGTGGGCTAACAGCGCGGGCACCAGGACGGACTCGATGAtcta                                                                               >  8:422749/1‑137 (MQ=255)
                                                              atatCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGATTC‑GGTTTTTGTACTTCTGGCGCGGGAGGGTGCGGATGAG‑TAAAGGTGTAGTGGCATAACAGAGCGGCAACACGGACGTACTCGATGATATCtt                                                                             >  1:156408/1‑140 (MQ=255)
                                                                    cTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAG‑TAAAGGTGTCGTGGCATAAAAGCGCGGCAAAACGGACGTACTCGATGATCTCTTCAAGCg                                                                       >  2:631934/1‑140 (MQ=255)
                                                                     tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGGCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                      <  4:131342/140‑1 (MQ=255)
                                                                     tCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGa                                                                      <  3:327629/140‑1 (MQ=255)
                                                                      cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCgatg                                                                                    <  7:85419/125‑1 (MQ=255)
                                                                      cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGAc                                                                     <  3:307792/140‑1 (MQ=255)
                                                                      cATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACGTCTGGCGGGGGCGGTTGCGGATGAG‑TAAAGGTGTGGTGGGATAACAGCGCGGCCACCCGGCCGTACTCGATGCTAGCGTCAAGCgcc                                                                     >  4:391689/1‑138 (MQ=255)
                                                                       aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGGGCGGGCGGTTGCGGATGAG‑TAAAGGTTTCGTGGGATAACAGCGCGGGCACACGGACGTACTCGAGTATCTCTTCAAGCGACa                                                                    >  4:366981/1‑140 (MQ=255)
                                                                       aTAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTGTTTGTAATTCTGGGGCGGTCGGTTGCGGCTGAG‑TAAAGGGGTCGTGGGCTAACAGCGCGGCCCCCCGGCCGTACGCGATGATCTCTTCACGCGCCa                                                                    >  4:613935/1‑140 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGCGTC‑GGTTTTTTTAATTCAGGCGCGGTCGGGTGCCGAAGTG‑TTAAGTTGTCGTGGCCTAACAGCGCGGCAAAACCGACGGACTCGCTGAACTCATCAAGctaaa                                                                    >  8:84063/1‑134 (MQ=255)
                                                                         aaCACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTTATTCTTGCGCGGTGGGGTTCGGTTTAG‑TAAAGGTGTCGTGGCATAACAACGCGGCAACACGGACGTTCTAGATGGTATCTTGAAGCGACAtt                                                                  >  5:546508/1‑140 (MQ=255)
                                                                              tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                             <  3:75827/140‑1 (MQ=255)
                                                                              tctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAg                                                             <  2:618029/140‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCaa                                                              <  7:569912/138‑1 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTTTTCTTCTGGGGCGGTCGGGTGCGGTTGAG‑TTAAAGTGTCGTGGGATAACAGCGCGGCAAAACCGAAGTACACGATGATCTCATCAAGCGACATTTCAAGc                                                            >  3:36936/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTTCTTATGGCGCGGGCGGGTGCGGATGAG‑TAAAGGTGTGGTGGCATAACAGCCCGGGAACACCGACCTACTCGATGCTCTCTTAAAGCCACATTTAAAGc                                                            >  2:217153/1‑140 (MQ=255)
                                                                               ctcCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTATGGCGCGGTCGGTTGGGGGTGAG‑TAAAGGTGTGGTGGGATAACAGCGCGGCAACAACGACGTAATCGATTCTCTCTTAAAGCGACAATTAAAGc                                                            >  5:508729/1‑140 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTTTACTTCTGGCGCGGTGGGTTGCGGTTGAG‑TAAAGGTGTGGTGGCAAAACACCGCGGCAACCCGGACCTACTCCAAGAAATCATCAAGAGACAATTAAAACTCttac                                                      >  6:324826/1‑138 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGGGGTCGGTTGGGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTTCTCGATGTTATATTCAAGCGACATTTAAAGCTGttac                                                      >  6:328261/1‑138 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                      <  5:184661/140‑1 (MQ=255)
                                                                                     aCATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTATGGCGCGGGCGGGTGGGGATGAG‑TAAAGGTGTGGTGGGATAACAGCGCGGCAACACGGACGGACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTcc                                                      >  1:283495/1‑140 (MQ=255)
                                                                                            ttGTTTTTAGTGTAGAGTC‑GGGTTTTGTACTTCTGGCGCGGTCGGGTGGGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGCCGTACACGATGATCTCTTAAAGCGCCATTTAAAGCTCGTCCTGAta                                                 >  8:508123/1‑137 (MQ=255)
                                                                                                   tAGTGTAGAGTC‑GGTTTTTGTACTTGTGGGGCGGTCGGGTGGGGATGAG‑GAAAGGTGTCGTGGCATAACCAGGCGGCCACACGGACGTACTCGATGCTCGCATCAAGCGACATTTCAATCTCATGCTGGtgttcgttt                                          >  8:118037/1‑136 (MQ=255)
                                                                                                     gtgtAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgtcttc                                        <  7:499003/138‑1 (MQ=255)
                                                                                                     gtgtAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                      <  3:523523/140‑1 (MQ=255)
                                                                                                       gtAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATcttcgtcttcgt                                      <  7:286166/138‑1 (MQ=255)
                                                                                                                ggcttttGTACTTCTGGCGCGGTCGGTTGAGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACTGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAAccc                              <  8:476641/135‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCTGTCGGTTGCTGATGAG‑TAAAGGTGTCGTTGCATACCAGCGCGGCAACACGTACGTACTCGATGATCTCTTCTAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGGCGTAACCCAAGTgag                       <  4:187599/140‑3 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  3:189886/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  1:133201/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  5:272201/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  1:689871/140‑1 (MQ=255)
                                                                                                                     ttGTACTTCTGGCGCGGTCGGTTGCGGAGGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTg                         <  7:627169/138‑1 (MQ=255)
                                                                                                                       gTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcg                       <  7:508123/138‑1 (MQ=255)
                                                                                                                       gTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGcgcg                     <  6:437824/140‑1 (MQ=255)
                                                                                                                       gTACTTCTGGCGCGGGCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcgct                     <  3:176908/140‑2 (MQ=255)
                                                                                                                        tACTTCTGGCGCGGGCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACTGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTgcgc                      <  8:656044/138‑1 (MQ=255)
                                                                                                                            tCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt                >  3:624358/1‑140 (MQ=255)
                                                                                                                             cTGGCGCGGTCGGTTGGGGTTGAG‑TTAAGGGGTCGTGGGATTACAGCGCGGCTACTCGGTGGTACTCGATGATCTCTTCTAGCGACATTTCAAGCTCTTCCTGATCTTGGTCTTCGTCGTTCCCGATCTGCGCGATGTTa               <  4:581597/140‑1 (MQ=255)
                                                                                                                             cTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTa               <  1:76960/140‑1 (MQ=255)
                                                                                                                              tGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGtt                >  7:451937/1‑138 (MQ=255)
                                                                                                                              tGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATATTCGTCTTCGTCGTAACCCAACTGCGCGATGtt                >  7:201008/1‑138 (MQ=255)
                                                                                                                               ggCGCTGTAGGTTGCGGATGAG‑TAAAGTTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGTTTATCTCTTCAAGCGACATTTCAAGCTCTTCCTTATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg             <  6:601054/140‑1 (MQ=255)
                                                                                                                               ggCGCGGGCGGGTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACg             <  5:324826/140‑1 (MQ=255)
                                                                                                                                   gggtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCACGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGa         <  3:64279/139‑1 (MQ=255)
                                                                                                                                   cggtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCa           <  7:161276/138‑1 (MQ=255)
                                                                                                                                     gtcggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc       >  5:78462/1‑140 (MQ=255)
                                                                                                                                       cggtTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGAtc       >  8:392333/1‑138 (MQ=255)
                                                                                                                                          ttGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCGa  <  3:467633/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CGCTGCCGGGTTGCATTTGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATATCACTCATAACACTCTCCTTACATTTTTTGTTTTTAGTGTAGAGTC‑GGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAG‑TAAAGGTGTCGTGGCATAACAGCGCGGCAACACGGACGTACTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAACTGCGCGATGTTACGCAGATCGTCGA  >  NZ_CP022686/1674111‑1674387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A