breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 178 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,264,3320AT53.8% 18.9 / 40.5 26I238F (ATT→TTT) kdsA3‑deoxy‑8‑phosphooctulonate synthase
*NZ_CP0092731,995,3990AC51.1% 52.5 / 74.6 45intergenic (‑129/+192)fliA/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NZ_CP0092734,277,6380GA46.0% 42.5 / 31.4 50intergenic (‑339/‑54)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NZ_CP0092732,897,0510CA44.9% 76.6 / 28.3 49intergenic (+318/+1055)ygcE/queEFGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NZ_CP0092731,995,3920GA43.8% 49.5 / 25.0 48intergenic (‑122/+199)fliA/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NZ_CP0092731,009,4880AT42.5% 57.6 / 17.1 41L258F (TTA→TTTpqiBintermembrane transport protein PqiB
*NZ_CP0092731,264,3350GT42.3% 21.6 / 10.2 26E239* (GAA→TAA) kdsA3‑deoxy‑8‑phosphooctulonate synthase
*NZ_CP0092734,594,1670CT41.7% 77.4 / 23.0 60S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092732,089,5710AG41.3% 60.8 / 12.5 47I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092732,551,8900AC40.6% 124.4 / 13.6 69V50G (GTG→GGG) eutBethanolamine ammonia‑lyase subunit alpha
*NZ_CP0092734,594,1610CT40.0% 77.2 / 24.8 60S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092734,091,4350TG39.7% 118.3 / 11.2 68intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase [Mn]/2‑keto‑3‑deoxygluconate transporter
*NZ_CP0092732,997,2800TG39.7% 100.3 / 11.0 59Y145D (TAT→GAT) xdhCxanthine dehydrogenase iron sulfur‑binding subunit XdhC
*NZ_CP0092733,753,7440AC39.6% 51.1 / 16.8 48V289G (GTG→GGG) selAL‑seryl‑tRNA(Sec) selenium transferase
*NZ_CP0092734,277,6470TA39.6% 59.4 / 31.4 48intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NZ_CP0092731,217,6890GA39.1% 73.3 / 12.5 46pseudogene (2228/2243 nt)BW25113_RS25990autotransporter outer membrane beta‑barrel domain‑containing protein
*NZ_CP0092734,091,4420GT38.8% 85.5 / 35.1 67intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase [Mn]/2‑keto‑3‑deoxygluconate transporter
*NZ_CP009273937,1690TG38.3% 78.7 / 16.8 47V252G (GTG→GGG) dmsAdimethylsulfoxide reductase subunit A
*NZ_CP0092732,897,0740GA38.3% 64.8 / 31.1 49intergenic (+341/+1032)ygcE/queEFGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NZ_CP0092733,924,0780TG37.9% 104.6 / 15.8 68T126P (ACC→CCC) ravAATPase RavA

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 1203246 =55 (0.860)20 (0.330) 20/464 4.1 25.7% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 64 (1.070)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 453250771 (1.110)15 (0.240) 15/478 5.8 20.9% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 45 (0.730)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 4532499 =68 (1.060)14 (0.230) 13/478 6.4 20.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 45 (0.730)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 120326157 (0.890)13 (0.220) 12/464 6.6 18.1% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 64 (1.070)coding (57/540 nt) BW25113_RS06005 phage tail protein