breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 273 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,515,909 | 0 | A | C | 60.0% | 33.3 / 129.0 | 105 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 720,466 | 0 | G | C | 50.0% | 32.0 / 41.8 | 48 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 433 | 0 | T | G | 48.6% | 119.2 / 59.8 | 109 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 4,180,249 | 0 | T | G | 46.6% | 217.8 / 85.9 | 163 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 430,318 | 0 | T | C | 46.4% | ‑6.5 / 13.5 | 28 | D157G (GAC→GGC) | insL‑2 | putative transposase insL for insertion sequence IS186 |
* | REL606 | 1,854,401 | 0 | A | C | 43.6% | 158.2 / 63.8 | 126 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 1,135,991 | 0 | G | C | 42.3% | 38.6 / 10.7 | 26 | G23G (GGC→GGG) | dinI | DNA damage‑inducible protein I |
* | REL606 | 3,472,700 | 0 | C | G | 40.9% | 97.9 / 104.4 | 110 | A290G (GCG→GGG) | yhgA | predicted transposase |
* | REL606 | 4,392,556 | 0 | C | T | 39.6% | 252.5 / 151.4 | 225 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 4,111,429 | 0 | T | G | 39.2% | 171.3 / 18.7 | 102 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 4,320,225 | 0 | T | G | 38.3% | 396.6 / 147.4 | 235 | T40P (ACC→CCC) | melR | DNA‑binding transcriptional dual regulator |
* | REL606 | 1,853,067 | 0 | A | C | 37.7% | 288.9 / 145.5 | 191 | T56P (ACC→CCC) | yeaN | predicted transporter |
* | REL606 | 895,856 | 0 | G | C | 37.5% | 61.6 / 29.0 | 40 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
* | REL606 | 3,774,914 | 0 | C | G | 37.5% | 424.5 / 204.5 | 243 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 254,043 | 0 | T | G | 36.7% | 156.9 / 24.8 | 90 | pseudogene (588/1710 nt) | fhiA | b0229; flagellar system protein |
* | REL606 | 3,378,885 | 0 | T | G | 36.6% | 696.5 / 99.8 | 336 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
* | REL606 | 4,627,155 | 0 | A | G | 35.9% | 208.1 / 67.3 | 117 | pseudogene (224/759 nt) | creD | tolerance to colicin E2; b4400_1 |
* | REL606 | 3,344,466 | 0 | T | G | 35.2% | 681.6 / 112.5 | 321 | F515V (TTT→GTT) | acrF | multidrug efflux system protein |
* | REL606 | 1,759,444 | 0 | A | C | 34.4% | 470.9 / 29.5 | 195 | H261P (CAC→CCC) | ydiS | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
* | REL606 | 1,061,648 | 0 | A | C | 34.2% | 535.1 / 50.6 | 246 | G40G (GGT→GGG) | yccY | phosphotyrosine‑protein phosphatase |
Marginal new junction evidence (lowest skew 10 of 63 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2040188 = | 80 (0.250) | 10 (0.030) | 10/138 | 17.4 | 10.6% | intergenic (‑60/+83) | vioB/vioA | VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2040424 = | 95 (0.330) | coding (960/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | 2680743 = | 243 (0.770) | 9 (0.030) | 8/138 | 18.5 | 4.0% | coding (269/897 nt) | ECB_02511 | ParB family protein |
? | REL606 | 2681028 = | 206 (0.710) | coding (554/897 nt) | ECB_02511 | ParB family protein | |||||
* | ? | REL606 | = 3025591 | 317 (1.000) | 12 (0.040) | 7/144 | 19.5 | 4.1% | coding (1212/1377 nt) | ECB_02826 | hypothetical protein |
? | REL606 | = 3025818 | 255 (0.840) | coding (985/1377 nt) | ECB_02826 | hypothetical protein | |||||
* | ? | REL606 | 2042218 = | 144 (0.450) | 8 (0.030) | 6/134 | 19.5 | 5.6% | coding (603/1428 nt) | wzx | O antigen translocase (Wzx) |
? | REL606 | 2042455 = | 141 (0.500) | coding (366/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 2041682 = | 96 (0.300) | 5 (0.020) | 5/128 | 19.7 | 4.5% | coding (1139/1428 nt) | wzx | O antigen translocase (Wzx) |
? | REL606 | 2041834 = | 131 (0.480) | coding (987/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 665771 = | NA (NA) | 8 (0.030) | 6/138 | 19.7 | NA | noncoding (1084/1443 nt) | IS150 | repeat region |
? | REL606 | 666019 = | NA (NA) | noncoding (1332/1443 nt) | IS150 | repeat region | |||||
* | ? | REL606 | 2417500 = | 240 (0.760) | 7 (0.020) | 6/142 | 20.0 | 2.7% | coding (67/1164 nt) | emrK | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
? | REL606 | 2417755 = | 274 (0.910) | intergenic (‑189/‑227) | emrK/evgA | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component/DNA‑binding response regulator in two‑component regulatory system with EvgS | |||||
* | ? | REL606 | 2041220 = | 105 (0.330) | 6 (0.020) | 6/142 | 20.0 | 7.3% | coding (164/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2041465 = | 54 (0.180) | coding (1356/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | = 3021002 | 250 (0.790) | 6 (0.020) | 5/136 | 20.3 | 2.8% | coding (482/933 nt) | ECB_02820 | hypothetical protein |
? | REL606 | = 3021158 | 192 (0.670) | coding (326/933 nt) | ECB_02820 | hypothetical protein | |||||
* | ? | REL606 | = 796784 | 208 (0.650) | 5 (0.020) | 5/138 | 20.4 | 2.7% | coding (30/1338 nt) | ECB_00738 | hypothetical protein |
? | REL606 | = 797024 | 164 (0.560) | coding (270/1338 nt) | ECB_00738 | hypothetical protein |