breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 273 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC60.0% 33.3 / 129.0 105V260G (GTG→GGG) ypfIpredicted hydrolase
*REL606720,4660GC50.0% 32.0 / 41.8 48A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6064330TG48.6% 119.2 / 59.8 109V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6064,180,2490TG46.6% 217.8 / 85.9 163G24G (GGT→GGGyjaHhypothetical protein
*REL606430,3180TC46.4% ‑6.5 / 13.5 28D157G (GAC→GGC) insL‑2putative transposase insL for insertion sequence IS186
*REL6061,854,4010AC43.6% 158.2 / 63.8 126K88N (AAA→AACyeaOhypothetical protein
*REL6061,135,9910GC42.3% 38.6 / 10.7 26G23G (GGC→GGGdinIDNA damage‑inducible protein I
*REL6063,472,7000CG40.9% 97.9 / 104.4 110A290G (GCG→GGG) yhgApredicted transposase
*REL6064,392,5560CT39.6% 252.5 / 151.4 225L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6064,111,4290TG39.2% 171.3 / 18.7 102G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6064,320,2250TG38.3% 396.6 / 147.4 235T40P (ACC→CCC) melRDNA‑binding transcriptional dual regulator
*REL6061,853,0670AC37.7% 288.9 / 145.5 191T56P (ACC→CCC) yeaNpredicted transporter
*REL606895,8560GC37.5% 61.6 / 29.0 40A26P (GCC→CCC) ECB_00836putative Regulatory protein
*REL6063,774,9140CG37.5% 424.5 / 204.5 243A20P (GCC→CCC) yicJpredicted transporter
*REL606254,0430TG36.7% 156.9 / 24.8 90pseudogene (588/1710 nt)fhiAb0229; flagellar system protein
*REL6063,378,8850TG36.6% 696.5 / 99.8 336T129P (ACC→CCC) rplB50S ribosomal protein L2
*REL6064,627,1550AG35.9% 208.1 / 67.3 117pseudogene (224/759 nt)creDtolerance to colicin E2; b4400_1
*REL6063,344,4660TG35.2% 681.6 / 112.5 321F515V (TTT→GTT) acrFmultidrug efflux system protein
*REL6061,759,4440AC34.4% 470.9 / 29.5 195H261P (CAC→CCC) ydiSpredicted oxidoreductase with FAD/NAD(P)‑binding domain
*REL6061,061,6480AC34.2% 535.1 / 50.6 246G40G (GGT→GGGyccYphosphotyrosine‑protein phosphatase

Marginal new junction evidence (lowest skew 10 of 63 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2040188 =80 (0.250)10 (0.030) 10/138 17.4 10.6% intergenic (‑60/+83) vioB/vioA VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2040424 = 95 (0.330)coding (960/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2680743 =243 (0.770)9 (0.030) 8/138 18.5 4.0% coding (269/897 nt) ECB_02511 ParB family protein
?REL606 2681028 = 206 (0.710)coding (554/897 nt) ECB_02511 ParB family protein
* ? REL606 = 3025591317 (1.000)12 (0.040) 7/144 19.5 4.1% coding (1212/1377 nt) ECB_02826 hypothetical protein
?REL606 = 3025818 255 (0.840)coding (985/1377 nt) ECB_02826 hypothetical protein
* ? REL606 2042218 =144 (0.450)8 (0.030) 6/134 19.5 5.6% coding (603/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2042455 = 141 (0.500)coding (366/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2041682 =96 (0.300)5 (0.020) 5/128 19.7 4.5% coding (1139/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2041834 = 131 (0.480)coding (987/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 665771 =NA (NA)8 (0.030) 6/138 19.7 NA noncoding (1084/1443 nt) IS150 repeat region
?REL606 666019 = NA (NA)noncoding (1332/1443 nt) IS150 repeat region
* ? REL606 2417500 =240 (0.760)7 (0.020) 6/142 20.0 2.7% coding (67/1164 nt) emrK EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
?REL606 2417755 = 274 (0.910)intergenic (‑189/‑227) emrK/evgA EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component/DNA‑binding response regulator in two‑component regulatory system with EvgS
* ? REL606 2041220 =105 (0.330)6 (0.020) 6/142 20.0 7.3% coding (164/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041465 = 54 (0.180)coding (1356/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 3021002250 (0.790)6 (0.020) 5/136 20.3 2.8% coding (482/933 nt) ECB_02820 hypothetical protein
?REL606 = 3021158 192 (0.670)coding (326/933 nt) ECB_02820 hypothetical protein
* ? REL606 = 796784208 (0.650)5 (0.020) 5/138 20.4 2.7% coding (30/1338 nt) ECB_00738 hypothetical protein
?REL606 = 797024 164 (0.560)coding (270/1338 nt) ECB_00738 hypothetical protein