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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 63 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,768,240 | 0 | C | T | 74.2% | 302.2 / 110.1 | 222 | A720A (GCG→GCA) | ydiJ | predicted FAD‑linked oxidoreductase |
* | W3110S.gb | 215,677 | 0 | G | C | 74.2% | 473.6 / 180.0 | 283 | A137P (GCG→CCG) | nlpE | lipoprotein involved with copper homeostasis and adhesion |
* | W3110S.gb | 1,768,246 | 0 | G | A | 73.9% | 287.9 / 113.5 | 222 | S718S (AGC→AGT) | ydiJ | predicted FAD‑linked oxidoreductase |
* | W3110S.gb | 4,290,958 | 0 | G | A | 73.5% | 627.1 / 245.8 | 464 | S2S (AGC→AGT) | acs | bifunctional acetyl‑CoA synthetase and propionyl‑CoA synthetase |
* | W3110S.gb | 3,946,914 | 0 | C | G | 73.5% | 435.6 / 179.8 | 276 | S578S (TCC→TCG) | bcsA | cellulose synthase, catalytic subunit |
* | W3110S.gb | 3,996,221 | 0 | G | A | 73.5% | 298.6 / 116.5 | 226 | L329L (TTG→TTA) | prlC | oligopeptidase A |
* | W3110S.gb | 872,063 | 0 | T | C | 73.3% | 333.8 / 120.6 | 240 | R225R (CGT→CGC) | yliC | predicted peptide transporter subunit |
* | W3110S.gb | 3,946,908 | 0 | C | T | 73.3% | 356.9 / 140.6 | 274 | T576T (ACC→ACT) | bcsA | cellulose synthase, catalytic subunit |
* | W3110S.gb | 3,996,224 | 0 | G | A | 73.0% | 274.1 / 117.6 | 226 | Q330Q (CAG→CAA) | prlC | oligopeptidase A |
* | W3110S.gb | 2,407,460 | 0 | G | A | 72.9% | 308.2 / 127.0 | 240 | F356F (TTC→TTT) | nuoC | NADH:ubiquinone oxidoreductase, chain C,D |
* | W3110S.gb | 1,768,252 | 0 | G | A | 72.8% | 270.6 / 114.1 | 213 | V716V (GTC→GTT) | ydiJ | predicted FAD‑linked oxidoreductase |
* | W3110S.gb | 2,985,114 | 0 | C | T | 71.5% | 207.4 / 96.8 | 172 | A204A (GCC→GCT) | yqeG | predicted transporter |
* | W3110S.gb | 554,190 | 0 | A | C | 70.4% | 450.2 / 194.2 | 284 | A119A (GCA→GCC) | cysS | cysteinyl‑tRNA synthetase |
* | W3110S.gb | 2,623,238 | 0 | A | G | 68.3% | 264.4 / 149.1 | 240 | L513L (CTA→CTG) | ppk | polyphosphate kinase, component of RNA degradosome |
* | W3110S.gb | 2,623,262 | 0 | G | A | 68.2% | 237.9 / 153.6 | 236 | E521E (GAG→GAA) | ppk | polyphosphate kinase, component of RNA degradosome |
* | W3110S.gb | 756,655 | 0 | C | T | 68.1% | 207.7 / 136.8 | 208 | L109L (CTC→CTT) | sdhA | succinate dehydrogenase, flavoprotein subunit |
* | W3110S.gb | 2,622,872 | 0 | T | C | 67.9% | 81.3 / 48.3 | 81 | H391H (CAT→CAC) | ppk | polyphosphate kinase, component of RNA degradosome |
* | W3110S.gb | 625,715 | 0 | T | A | 67.8% | 276.4 / 182.9 | 214 | T141T (ACT→ACA) | entE | 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex |
* | W3110S.gb | 625,718 | 0 | C | T | 67.8% | 212.6 / 143.0 | 214 | F142F (TTC→TTT) | entE | 2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex |
* | W3110S.gb | 3,618,848 | 0 | G | A | 67.6% | 317.2 / 225.3 | 331 | R167R (CGC→CGT) | rmuC | predicted recombination limiting protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | W3110S.gb | = 1301040 | 0 (0.000) | 163 (3.570) | 3/94 | 999999.0 | 100% | intergenic (+218/‑90) | ychE/insC | predicted inner membrane protein/IS2 element protein |
? | W3110S.gb | 1302377 = | 0 (0.000) | intergenic (+19/‑519) | insD/oppA | IS2 element protein/oligopeptide transporter subunit |