breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 63 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,768,2400CT74.2% 302.2 / 110.1 222A720A (GCG→GCAydiJpredicted FAD‑linked oxidoreductase
*W3110S.gb215,6770GC74.2% 473.6 / 180.0 283A137P (GCG→CCG) nlpElipoprotein involved with copper homeostasis and adhesion
*W3110S.gb1,768,2460GA73.9% 287.9 / 113.5 222S718S (AGC→AGTydiJpredicted FAD‑linked oxidoreductase
*W3110S.gb4,290,9580GA73.5% 627.1 / 245.8 464S2S (AGC→AGTacsbifunctional acetyl‑CoA synthetase and propionyl‑CoA synthetase
*W3110S.gb3,946,9140CG73.5% 435.6 / 179.8 276S578S (TCC→TCGbcsAcellulose synthase, catalytic subunit
*W3110S.gb3,996,2210GA73.5% 298.6 / 116.5 226L329L (TTG→TTAprlColigopeptidase A
*W3110S.gb872,0630TC73.3% 333.8 / 120.6 240R225R (CGT→CGCyliCpredicted peptide transporter subunit
*W3110S.gb3,946,9080CT73.3% 356.9 / 140.6 274T576T (ACC→ACTbcsAcellulose synthase, catalytic subunit
*W3110S.gb3,996,2240GA73.0% 274.1 / 117.6 226Q330Q (CAG→CAAprlColigopeptidase A
*W3110S.gb2,407,4600GA72.9% 308.2 / 127.0 240F356F (TTC→TTTnuoCNADH:ubiquinone oxidoreductase, chain C,D
*W3110S.gb1,768,2520GA72.8% 270.6 / 114.1 213V716V (GTC→GTTydiJpredicted FAD‑linked oxidoreductase
*W3110S.gb2,985,1140CT71.5% 207.4 / 96.8 172A204A (GCC→GCTyqeGpredicted transporter
*W3110S.gb554,1900AC70.4% 450.2 / 194.2 284A119A (GCA→GCCcysScysteinyl‑tRNA synthetase
*W3110S.gb2,623,2380AG68.3% 264.4 / 149.1 240L513L (CTA→CTGppkpolyphosphate kinase, component of RNA degradosome
*W3110S.gb2,623,2620GA68.2% 237.9 / 153.6 236E521E (GAG→GAAppkpolyphosphate kinase, component of RNA degradosome
*W3110S.gb756,6550CT68.1% 207.7 / 136.8 208L109L (CTC→CTTsdhAsuccinate dehydrogenase, flavoprotein subunit
*W3110S.gb2,622,8720TC67.9% 81.3 / 48.3 81H391H (CAT→CACppkpolyphosphate kinase, component of RNA degradosome
*W3110S.gb625,7150TA67.8% 276.4 / 182.9 214T141T (ACT→ACAentE2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex
*W3110S.gb625,7180CT67.8% 212.6 / 143.0 214F142F (TTC→TTTentE2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex
*W3110S.gb3,618,8480GA67.6% 317.2 / 225.3 331R167R (CGC→CGTrmuCpredicted recombination limiting protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 13010400 (0.000)163 (3.570) 3/94 999999.0 100% intergenic (+218/‑90) ychE/insC predicted inner membrane protein/IS2 element protein
?W3110S.gb 1302377 = 0 (0.000)intergenic (+19/‑519) insD/oppA IS2 element protein/oligopeptide transporter subunit