breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 204,511 C→T 100% intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G 100% intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 328,149 G→A 12.5% G98G (GGC→GGT aes ← acetyl esterase
RA 357,299 T→C 16.4% F5L (TTT→CTT)  sfmA → predicted fimbrial‑like adhesin protein
RA 1,005,755 G→C 7.1% intergenic (‑161/‑363) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/hypothetical protein
RA 1,036,505 G→A 100% A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,508,746 C→T 7.8% G125S (GGC→AGC)  yfbV ← conserved inner membrane protein
RA 1,668,051 G→A 7.4% Q378Q (CAG→CAA xseA → exonuclease VII, large subunit
RA 1,695,660 C→A 20.1% R117L (CGC→CTC)  yfhQ ← predicted methyltransferase
RA 1,736,586 G→T 7.4% A55E (GCG→GAG)  rnc ← RNase III
RA 1,754,163 G→A 9.8% A509T (GCG→ACG)  yfiQ → fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit
RA 1,763,556 T→C 30.7% noncoding (288/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,763,636 A→G 31.2% noncoding (208/1542 nt) rrsG ← 16S ribosomal RNA
RA 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 1,968,171 C→G 8.5% M153I (ATG→ATC) ‡ tktA ← transketolase 1, thiamin‑binding
RA 1,968,173 T→G 8.1% M153L (ATG→CTG) ‡ tktA ← transketolase 1, thiamin‑binding
RA 2,036,045 C→T 12.2% C59C (TGC→TGT ygiC → hypothetical protein
RA 2,063,055 T→C 5.8% R412R (CGT→CGC dnaG → DNA primase
RA 2,138,339 G→A 13.0% intergenic (‑68/+96) rbfA ← / ← infB 30s ribosome binding factor/fused protein chain initiation factor 2, IF2
RA 2,344,730 C→T 12.0% L466L (CTG→TTG)  glnG → fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)
RA 2,359,867 G→A 28.9% noncoding (1228/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,359,876 C→T 24.1% noncoding (1219/2905 nt) rrlA ← 23S ribosomal RNA
RA 2,548,960 C→T 13.5% L13L (CTG→TTG)  kbl → glycine C‑acetyltransferase
RA 2,776,458 A→G 9.8% Y410H (TAT→CAT)  arpA ← regulator of acetyl CoA synthetase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ minE 252002 252041 40 14 [12] [12] 13 malZ maltodextrin glucosidase
* * ÷ minE 444388 444443 56 14 [12] [12] 14 kdpE DNA‑binding response regulator in two‑component regulatory system with KdpD
* * ÷ minE 865550 865594 45 14 [11] [11] 14 yciQ predicted inner membrane protein
* * ÷ minE 899415 899454 40 15 [12] [12] 13 pspD peripheral inner membrane phage‑shock protein
* * ÷ minE 1420587 1420617 31 13 [10] [9] 14 ccmF heme lyase, CcmF subunit
* * ÷ minE 1616726 1616750 25 13 [11] [10] 13 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ minE 1847588 1847709 122 13 [10] [11] 13 iap/ygbF aminopeptidase in alkaline phosphatase isozyme conversion/hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE = 16117562 (0.040)30 (0.550)
+A
11/100 NT 49.7% coding (1397/1404 nt) eutA reactivating factor for ethanolamine ammonia lyase
?minE 2083040 = 60 (1.080)coding (66/663 nt) yqjA conserved inner membrane protein