breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 204,511 | C→T | 100% | intergenic (+111/‑73) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
RA | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
RA | 328,149 | G→A | 12.5% | G98G (GGC→GGT) | aes ← | acetyl esterase |
RA | 357,299 | T→C | 16.4% | F5L (TTT→CTT) | sfmA → | predicted fimbrial‑like adhesin protein |
RA | 1,005,755 | G→C | 7.1% | intergenic (‑161/‑363) | pntA ← / → ydgH | pyridine nucleotide transhydrogenase, alpha subunit/hypothetical protein |
RA | 1,036,505 | G→A | 100% | A632A (GCG→GCA) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
RA | 1,508,746 | C→T | 7.8% | G125S (GGC→AGC) | yfbV ← | conserved inner membrane protein |
RA | 1,668,051 | G→A | 7.4% | Q378Q (CAG→CAA) | xseA → | exonuclease VII, large subunit |
RA | 1,695,660 | C→A | 20.1% | R117L (CGC→CTC) | yfhQ ← | predicted methyltransferase |
RA | 1,736,586 | G→T | 7.4% | A55E (GCG→GAG) | rnc ← | RNase III |
RA | 1,754,163 | G→A | 9.8% | A509T (GCG→ACG) | yfiQ → | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
RA | 1,763,556 | T→C | 30.7% | noncoding (288/1542 nt) | rrsG ← | 16S ribosomal RNA |
RA | 1,763,636 | A→G | 31.2% | noncoding (208/1542 nt) | rrsG ← | 16S ribosomal RNA |
RA | 1,892,019:1 | +GC | 100% | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
RA | 1,968,171 | C→G | 8.5% | M153I (ATG→ATC) ‡ | tktA ← | transketolase 1, thiamin‑binding |
RA | 1,968,173 | T→G | 8.1% | M153L (ATG→CTG) ‡ | tktA ← | transketolase 1, thiamin‑binding |
RA | 2,036,045 | C→T | 12.2% | C59C (TGC→TGT) | ygiC → | hypothetical protein |
RA | 2,063,055 | T→C | 5.8% | R412R (CGT→CGC) | dnaG → | DNA primase |
RA | 2,138,339 | G→A | 13.0% | intergenic (‑68/+96) | rbfA ← / ← infB | 30s ribosome binding factor/fused protein chain initiation factor 2, IF2 |
RA | 2,344,730 | C→T | 12.0% | L466L (CTG→TTG) | glnG → | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
RA | 2,359,867 | G→A | 28.9% | noncoding (1228/2905 nt) | rrlA ← | 23S ribosomal RNA |
RA | 2,359,876 | C→T | 24.1% | noncoding (1219/2905 nt) | rrlA ← | 23S ribosomal RNA |
RA | 2,548,960 | C→T | 13.5% | L13L (CTG→TTG) | kbl → | glycine C‑acetyltransferase |
RA | 2,776,458 | A→G | 9.8% | Y410H (TAT→CAT) | arpA ← | regulator of acetyl CoA synthetase |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | minE | 252002 | 252041 | 40 | 14 [12] | [12] 13 | malZ | maltodextrin glucosidase |
* | * | ÷ | minE | 444388 | 444443 | 56 | 14 [12] | [12] 14 | kdpE | DNA‑binding response regulator in two‑component regulatory system with KdpD |
* | * | ÷ | minE | 865550 | 865594 | 45 | 14 [11] | [11] 14 | yciQ | predicted inner membrane protein |
* | * | ÷ | minE | 899415 | 899454 | 40 | 15 [12] | [12] 13 | pspD | peripheral inner membrane phage‑shock protein |
* | * | ÷ | minE | 1420587 | 1420617 | 31 | 13 [10] | [9] 14 | ccmF | heme lyase, CcmF subunit |
* | * | ÷ | minE | 1616726 | 1616750 | 25 | 13 [11] | [10] 13 | eutE | predicted aldehyde dehydrogenase, ethanolamine utilization protein |
* | * | ÷ | minE | 1847588 | 1847709 | 122 | 13 [10] | [11] 13 | iap/ygbF | aminopeptidase in alkaline phosphatase isozyme conversion/hypothetical protein |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | minE | = 1611756 | 2 (0.040) | 30 (0.550) +A |
11/100 | NT | 49.7% | coding (1397/1404 nt) | eutA | reactivating factor for ethanolamine ammonia lyase |
? | minE | 2083040 = | 60 (1.080) | coding (66/663 nt) | yqjA | conserved inner membrane protein |