breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 224,699 (G)5→4 noncoding (929/1542 nt) rrsH → 16S ribosomal RNA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 319,759 C→A K81N (AAG→AAT rclA ← putative pyridine nucleotide‑disulfide oxidoreductase RclA
RA 331,384 A→G Y641C (TAC→TGC)  betT → choline:H(+) symporter
RA 539,260 C→A G38G (GGC→GGA allB → allantoinase
RA 720,542 A→G intergenic (‑82/‑41) speF ← / → ybfK ornithine decarboxylase, degradative/uncharacterized protein YbfK
RA 793,209 G→T R194S (CGC→AGC)  modF ← ABC family protein ModF
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,185,198 G→T R133R (CGG→AGG)  potA ← spermidine preferential ABC transporter ATP binding subunit
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,399,674 T→C intergenic (‑148/+47) fnr ← / ← ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,604,033 C→A R339R (CGG→AGG)  lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,619,336 C→A R73S (CGT→AGT)  marR → DNA‑binding transcriptional repressor MarR
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,494,132 T→C T91A (ACC→GCC)  yfdX ← protein YfdX
RA 2,749,752 G→T G327C (GGC→TGC)  yfjD → putative inner membrane protein
RA 2,759,763 A→T Q260L (CAG→CTG)  yfjI → CP4‑57 prophage; protein YfjI
RA 2,780,495 G→T G744G (GGC→GGA ypjA ← adhesin‑like autotransporter YpjA
RA 2,964,330 C→A intergenic (‑153/+31) ppdA ← / ← thyA conserved protein PpdA/thymidylate synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,447,873 G→T Q93K (CAG→AAG)  rplN ← 50S ribosomal subunit protein L14
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,726,892 C→A intergenic (+10/+32) wecH → / ← yiaA O‑acetyltransferase WecH/conserved inner membrane protein YiaA
RA 3,747,397 G→T R105L (CGG→CTG)  lyxK → L‑xylulose kinase
RA 3,758,721 C→T A381T (GCG→ACG)  selB ← selenocysteyl‑tRNA‑specific translation elongation factor
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,245,598 C→A P274P (CCG→CCT malE ← maltose ABC transporter periplasmic binding protein
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196304 1211416 15113 5 [3] [4] 5 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102995 3104048 1054 5 [4] [3] 5 [mutY] [mutY]
* * ÷ NC_000913 3131394 3131491 98 5 [3] [3] 5 yghQ putative transport protein YghQ
* * ÷ NC_000913 3423762–3424234 3424525–3424239 6–764 5 [4] [4] 5 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =41 (1.390)29 (0.990) 21/274 0.4 58.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)25 (0.850) 20/276 0.4 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA