breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 99,275 G→T L291L (CTG→CTT ftsW → putative lipid II flippase FtsW
RA 221,587 C→A G12V (GGC→GTC)  metI ← L‑methionine/D‑methionine ABC transporter membrane subunit
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 327,459 G→T N425K (AAC→AAA betB ← betaine aldehyde dehydrogenase
JC JC 563,949 IS1 (+) +9 bp coding (524‑532/633 nt) fimZ ← putative LuxR family transcriptional regulator FimZ
RA 779,704 G→T G36V (GGC→GTC)  cpoB → cell division coordinator CpoB
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,363,510 G→T A256A (GCG→GCT puuC → gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
RA 1,453,692 A→G intergenic (‑50/‑235) paaZ ← / → paaA oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase/phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
RA 1,506,188 G→T R6L (CGC→CTC)  sutR → DNA‑binding transcriptional dual regulator SutR
RA 1,581,256 G→T P423P (CCC→CCA ydeN ← putative sulfatase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
MC JC 1,689,711 Δ10 bp coding (1136‑1145/1176 nt) manA → mannose‑6‑phosphate isomerase
RA 1,798,037 C→A L302L (CTG→CTT pheS ← phenylalanine‑‑tRNA ligase subunit alpha
RA 1,825,636 C→A intergenic (‑11/+319) spy ← / ← astE ATP‑independent periplasmic chaperone/succinylglutamate desuccinylase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,349,048 Δ2 bp coding (424‑425/651 nt) inaA ← putative lipopolysaccharide kinase InaA
JC 2,812,166 Δ20 bp coding (740‑759/1173 nt) emrA → multidrug efflux pump membrane fusion protein EmrA
RA 2,906,534 G→T S275I (AGC→ATC)  ygcG → protein YgcG
RA 3,012,629 C→A V537V (GTG→GTT yqeB ← XdhC‑CoxI family protein YqeB
RA 3,242,322 G→T Y331* (TAC→TAA uxaA ← D‑altronate dehydratase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,788,257 G→T A53S (GCT→TCT)  yibQ → divergent polysaccharide deacetylase domain‑containing protein YibQ
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,903,152 C→A V184L (GTG→TTG)  bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,924,708 G→T P309Q (CCG→CAG)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 4,035,273 T→C intergenic (+120/‑258) hemG → / → rrsA protoporphyrinogen oxidase/16S ribosomal RNA
RA 4,058,369 T→C intergenic (‑335/‑38) glnA ← / → bipA glutamine synthetase/ribosome‑dependent GTPase, ribosome assembly factor
RA 4,061,400 C→A A79D (GCC→GAC)  yihM → putative TIM barrel domain‑containing protein YihM
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,392,580 C→A intergenic (+33/‑3) glyX → / → glyY tRNA‑Gly/tRNA‑Gly
RA 4,407,427 G→T A258A (GCG→GCT rnr → RNase R
RA 4,611,524 C→A V43V (GTC→GTA osmY → periplasmic chaperone OsmY
RA 4,616,518 A→G V183A (GTT→GCT)  yjjI ← DUF3029 domain‑containing protein YjjI

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211428 15154 33 [28] [28] 34 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102995 3104081 1087 29 [28] [28] 29 mutY mutY
* * ÷ NC_000913 3423764–3424234 3424534–3424238 5–771 29 [28] [28] 29 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)171 (1.630) 93/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1555804 =88 (0.820)115 (1.070) 55/276 0.5 49.1% intergenic (‑135/+22) maeA/sra malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22
?NC_000913 = 1556015 150 (1.390)intergenic (‑52/+50) sra/bdm 30S ribosomal subunit protein S22/biofilm‑dependent modulation protein
* ? NC_000913 3363001 =175 (1.620)166 (1.550) 73/274 0.1 65.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)125 (1.160) 73/276 0.1 99.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.010)coding (3907/4134 nt) rhsA rhs element protein RhsA