breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 36,087 A→G 27.1% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase
RA 36,119 C→T 14.2% V145I (GTA→ATA)  USA300HOU_0026 ← IS431mec transposase
RA 36,124 G→A 23.7% A143V (GCT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,161 T→C 10.8% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,174 T→C 12.5% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase
RA 36,315 G→A 10.5% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase
RA 36,378 C→T 14.7% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase
RA 36,413 C→T 9.5% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase
RA 76,295 T→C 16.2% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 100,683 G→A 8.1% intergenic (‑185/+244) USA300HOU_0099 ← / ← USA300HOU_0101 possible MFS family major facilitator transporter/hypothetical protein
RA 110,651 C→T 13.5% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 18.8% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 35.2% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 36.1% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 148,490 T→C 11.3% L105L (TTG→CTG)  galE → UDP‑glucose 4‑epimerase
RA 195,286 T→A 100% intergenic (‑97/‑82) USA300HOU_0183 ← / → USA300HOU_0184 hypothetical protein/hypothetical protein
RA 202,469 A→T 10.0% Q186L (CAA→CTA)  USA300HOU_0191 → MFS family major facilitator transporter, multidrug cation symporter or antiporter
RA 396,540 G→A 100% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein
RA 512,643 A→G 46.0% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 607,884 G→A 100% R209* (CGA→TGA)  USA300HOU_0550 ← deoxynucleoside kinase
RA 629,681 C→A 100% L84I (CTA→ATA)  USA300HOU_0564 → possible 3‑hexulose‑6‑phosphate synthase
RA 665,392 T→G 100% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase
RA 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
RA 1,233,504 T→A 100% V470D (GTT→GAT)  USA300HOU_1157 → non‑specific serine/threonine protein kinase
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,440,085 G→T 100% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
RA 1,486,271 T→C 9.3% T5251T (ACA→ACG ebh ← extracellular matrix binding protein
RA 1,499,075 G→A 9.2% N983N (AAC→AAT ebh ← extracellular matrix binding protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,717,160 C→A 11.1% intergenic (‑174/+152) USA300HOU_1609 ← / ← greA possible allophanate hydrolase subunit 1/elongation factor GreA
RA 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase
RA 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase
RA 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,352,815 G→A 100% A92V (GCA→GTA)  rpsI ← ribosomal protein S9
RA 2,454,626 A→T 100% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,756,899 T→C 7.5% intergenic (‑1/+99) USA300HOU_2612 ← / ← nrdG hypothetical protein/anaerobic ribonucleotide reductase small subunit

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 8240761 (1.540)3 (0.080) 3/250 NT 4.6% coding (512/957 nt) USA300HOU_0079 nickel (Ni2+)/peptide ABC superfamily ATP binding cassette transporter, membrane protein
?CP000730 = 82418 65 (1.710)coding (523/957 nt) USA300HOU_0079 nickel (Ni2+)/peptide ABC superfamily ATP binding cassette transporter, membrane protein
* ? CP000730 512620 =44 (1.110)26 (0.950)
+40 bp
24/180 NT 46.1% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 = 285454950 (1.260)3 (0.080) 3/254 NT 6.0% intergenic (+247/+166) USA300HOU_2696/rarD DASS family divalent anion:sodium (Na+) symporter/DMT superfamily drug/metabolite transporter
?CP000730 = 2854587 46 (1.190)intergenic (+285/+128) USA300HOU_2696/rarD DASS family divalent anion:sodium (Na+) symporter/DMT superfamily drug/metabolite transporter