breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,910 T→A W30R (TGG→AGG)  folA → type 3 dihydrofolate reductase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,303,883 A→C V215V (GTT→GTG pnp ← polyribonucleotide nucleotidyltransferase
RA 3,390,316 A→C L280R (CTG→CGG)  rng ← ribonuclease G
RA 3,877,246 A→T intergenic (‑157/‑140) dnaA ← / → BW25113_RS25780 chromosomal replication initiator protein DnaA/protein YsdD
RA 4,527,045 A→C S123A (TCG→GCG)  nanS ← 9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356968 357570 603 2 [1] [1] 6 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 410635 410688 54 2 [1] [1] 2 sbcC exonuclease subunit SbcC
* * ÷ NZ_CP009273 497865 497879 15 2 [0] [1] 2 ybaL Kef family K(+) transporter
* * ÷ NZ_CP009273 508530 508565 36 3 [1] [0] 2 ybaT APC family permease
* * ÷ NZ_CP009273 522948 522968 21 2 [1] [1] 3 rhsD rhs element protein RhsD
* * ÷ NZ_CP009273 533002 533049 48 3 [1] [1] 2 glxR/ybbW 2‑hydroxy‑3‑oxopropionate reductase/putative allantoin permease
* * ÷ NZ_CP009273 578977 579061 85 4 [1] [1] 2 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 603181 603190 10 2 [0] [1] 3 ybdK/hokE YbdK family carboxylate‑amine ligase/type I toxin‑antitoxin system toxin HokE
* * ÷ NZ_CP009273 613835 613873 39 2 [1] [1] 2 wzz(fepE) LPS O‑antigen length regulator Wzz(fepE)
* * ÷ NZ_CP009273 621109 621119 11 3 [1] [0] 2 entC isochorismate synthase EntC
* * ÷ NZ_CP009273 633099 633157 59 2 [1] [1] 2 ybdO/dsbG LysR family transcriptional regulator/thiol:disulfide interchange protein DsbG
* * ÷ NZ_CP009273 655290 655296 7 2 [0] [1] 3 lipA lipoyl synthase
* * ÷ NZ_CP009273 665072 665087 16 2 [1] [1] 2 cobC adenosylcobalamin/alpha‑ribazole phosphatase
* * ÷ NZ_CP009273 674902 674924 23 6 [1] [1] 2 ybeT/ybeU tetratricopeptide repeat protein/DUF1266 domain‑containing protein
* * ÷ NZ_CP009273 695918 695923 6 2 [0] [0] 2 nagC DNA‑binding transcriptional regulator NagC
* * ÷ NZ_CP009273 698795 698809 15 2 [1] [1] 4 nagB glucosamine‑6‑phosphate deaminase
* * ÷ NZ_CP009273 719386 719434 49 2 [0] [1] 2 kdpD two‑component system sensor histidine kinase KdpD
* * ÷ NZ_CP009273 733502 733512 11 5 [1] [1] 4 BW25113_RS03650/ybfD ISAs1 family transposase/ISAs1 family transposase
* * ÷ NZ_CP009273 748526 748536 11 2 [1] [0] 2 ybgD/gltA fimbrial protein/citrate synthase
* * ÷ NZ_CP009273 766335 766365 31 2 [0] [1] 2 mngB/cydA mannosylglycerate hydrolase/cytochrome ubiquinol oxidase subunit I
* * ÷ NZ_CP009273 790224 790249 26 2 [1] [1] 3 [acrZ] [acrZ]
* * ÷ NZ_CP009273 806077 806087 11 5 [1] [0] 2 bioF 8‑amino‑7‑oxononanoate synthase
* * ÷ NZ_CP009273 902756 902768 13 2 [0] [1] 3 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 902978 903087 110 3 [1] [0] 2 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 905464 905470 7 2 [1] [1] 2 poxB ubiquinone‑dependent pyruvate dehydrogenase
* * ÷ NZ_CP009273 906777 906816 40 2 [1] [1] 2 hcr NADH oxidoreductase
* * ÷ NZ_CP009273 930328 930478 151 2 [1] [1] 2 ftsK DNA translocase FtsK
* * ÷ NZ_CP009273 940576 940603 28 2 [1] [0] 2 ycaC hydrolase
* * ÷ NZ_CP009273 950047 950139 93 2 [1] [1] 2 focA/ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
* * ÷ NZ_CP009273 973658 973692 35 2 [1] [1] 2 mukB chromosome partition protein MukB
* * ÷ NZ_CP009273 982848 982918 71 2 [1] [1] 2 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase
* * ÷ NZ_CP009273 985316 985324 9 2 [0] [0] 2 pncB nicotinate phosphoribosyltransferase
* * ÷ NZ_CP009273 993098 993230 133 10 [0] [1] 2 ssuE/elfA NADPH‑dependent FMN reductase/fimbrial protein
* * ÷ NZ_CP009273 1010335 1010345 11 2 [1] [1] 2 pqiB intermembrane transport protein PqiB
* * ÷ NZ_CP009273 1027066 1027072 7 2 [1] [0] 2 yccA/BW25113_RS05040 FtsH protease modulator YccA/tRNA‑Ser
* * ÷ NZ_CP009273 1028371 1028386 16 2 [0] [1] 2 hyaA hydrogenase 1 small subunit
* * ÷ NZ_CP009273 1032742 1032773 32 2 [1] [1] 3 hyaF hydrogenase expression/formation protein
* * ÷ NZ_CP009273 1039031 1039043 13 3 [1] [1] 2 etk tyrosine‑protein kinase
* * ÷ NZ_CP009273 1098896 1099066 171 2 [1] [1] 2 csgD/csgB biofilm master transcriptional regulator CsgD/curli minor subunit CsgB
* * ÷ NZ_CP009273 1144185 1144229 45 4 [1] [1] 2 [fabH] [fabH]
* * ÷ NZ_CP009273 1147592 1147598 7 3 [0] [0] 2 fabF beta‑ketoacyl‑ACP synthase II
* * ÷ NZ_CP009273 1157988 1158024 37 3 [1] [1] 2 ycfL YcfL family protein
* * ÷ NZ_CP009273 1162014 1162029 16 2 [0] [0] 2 ndh NADH‑quinone dehydrogenase
* * ÷ NZ_CP009273 1172434 1172454 21 2 [1] [0] 2 lolD lipoprotein‑releasing ABC transporter ATP‑binding protein LolD
* * ÷ NZ_CP009273 1175205 1175235 31 2 [1] [0] 2 cobB NAD‑dependent protein deacylase
* * ÷ NZ_CP009273 1177347 1177372 26 2 [1] [0] 2 potD spermidine/putrescine ABC transporter substrate‑binding protein PotD
* * ÷ NZ_CP009273 1185154 1185166 13 2 [1] [1] 3 phoQ two‑component system sensor histidine kinase PhoQ
* * ÷ NZ_CP009273 1206476 1206510 35 2 [1] [1] 2 mcrA 5‑methylcytosine‑specific endonuclease McrA
* * ÷ NZ_CP009273 1207681 1207691 11 2 [0] [1] 3 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1208379 1208403 25 2 [1] [1] 2 ycgX DUF1398 domain‑containing protein
* * ÷ NZ_CP009273 1210016 1210038 23 2 [1] [1] 2 bluF blue light‑responsive regulator BluF
* * ÷ NZ_CP009273 1223311 1223385 75 2 [1] [1] 2 BW25113_RS06140 YcgL domain‑containing protein
* * ÷ NZ_CP009273 1246449 1246497 49 2 [1] [1] 2 dhaK/dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
* * ÷ NZ_CP009273 1250428 1250441 14 2 [1] [0] 2 ycgV autotransporter outer membrane beta‑barrel domain‑containing protein
* * ÷ NZ_CP009273 1251414 1251422 9 2 [1] [1] 2 ycgV/ychF autotransporter outer membrane beta‑barrel domain‑containing protein/redox‑regulated ATPase YchF
* * ÷ NZ_CP009273 1262318 1262351 34 3 [0] [1] 3 prmC peptide chain release factor N(5)‑glutamine methyltransferase
* * ÷ NZ_CP009273 1264427 1264513 87 2 [1] [1] 2 [kdsA] [kdsA]
* * ÷ NZ_CP009273 1285084 1285090 7 2 [1] [1] 2 rssA patatin‑like phospholipase RssA
* * ÷ NZ_CP009273 1285189 1285225 37 2 [1] [1] 3 rssA patatin‑like phospholipase RssA
* * ÷ NZ_CP009273 1302883 1302913 31 2 [1] [1] 4 [cls] [cls]
* * ÷ NZ_CP009273 1305995 1306027 33 4 [0] [1] 2 tonB TonB system transport protein TonB
* * ÷ NZ_CP009273 1306911 1306917 7 2 [0] [1] 2 yciB septation protein A
* * ÷ NZ_CP009273 1312462 1312500 39 2 [0] [1] 2 trpB tryptophan synthase subunit beta
* * ÷ NZ_CP009273 1320430 1320443 14 2 [1] [1] 3 yciQ DUF2207 domain‑containing protein
* * ÷ NZ_CP009273 1341088 1341115 28 2 [1] [1] 2 BW25113_RS25050 hypothetical protein
* * ÷ NZ_CP009273 1345140 1345167 28 2 [0] [1] 2 fabI enoyl‑ACP reductase FabI
* * ÷ NZ_CP009273 1349751 1349771 21 2 [1] [1] 3 sapA peptide ABC transporter substrate‑binding protein SapA
* * ÷ NZ_CP009273 1354220 1354253 34 2 [1] [1] 2 puuA glutamate‑putrescine ligase
* * ÷ NZ_CP009273 1360690 1360743 54 2 [1] [1] 2 puuE 4‑aminobutyrate transaminase
* * ÷ NZ_CP009273 1374683 1374693 11 2 [1] [1] 2 pgmB beta‑phosphoglucomutase
* * ÷ NZ_CP009273 1385374 1385393 20 3 [1] [1] 2 ycjY alpha/beta hydrolase
* * ÷ NZ_CP009273 1398033 1398035 3 2 [1] [0] 2 abgA p‑aminobenzoyl‑glutamate hydrolase subunit AbgA
* * ÷ NZ_CP009273 1411455 1411500 46 2 [1] [1] 3 recE exodeoxyribonuclease VIII
* * ÷ NZ_CP009273 1417969 1418045 77 2 [1] [0] 2 [trkG] [trkG]
* * ÷ NZ_CP009273 1421014 1421029 16 2 [1] [1] 3 ynaA phage tail length tape measure family protein
* * ÷ NZ_CP009273 1424060 1424146 87 2 [1] [0] 2 stfR prophage tail fiber N‑terminal domain‑containing protein
* * ÷ NZ_CP009273 1424264 1424323 60 2 [1] [1] 2 stfR prophage tail fiber N‑terminal domain‑containing protein
* * ÷ NZ_CP009273 1427977–1428243 1428279–1428260 18–303 2 [1] [1] 2 [ydfK] [ydfK]
* * ÷ NZ_CP009273 1431601 1431639 39 2 [0] [1] 2 nifJ pyruvate:ferredoxin (flavodoxin) oxidoreductase
* * ÷ NZ_CP009273 1436990 1437008 19 2 [1] [0] 2 ldhA D‑lactate dehydrogenase
* * ÷ NZ_CP009273 1444601 1444652 52 2 [1] [1] 2 tynA primary‑amine oxidase
* * ÷ NZ_CP009273 1453643 1453663 21 2 [1] [1] 2 paaC 3‑hydroxyacyl‑CoA dehydrogenase PaaC
* * ÷ NZ_CP009273 1459374 1459384 11 2 [1] [0] 2 paaY/BW25113_RS25580 phenylacetic acid degradation protein PaaY/hypothetical protein
* * ÷ NZ_CP009273 1494776 1494889 114 3 [1] [1] 2 [tehA] [tehA]
* * ÷ NZ_CP009273 1513768 1513823 56 2 [1] [1] 2 curA NADPH‑dependent curcumin/dihydrocurcumin reductase
* * ÷ NZ_CP009273 1524311 1524343 33 5 [1] [1] 2 BW25113_RS07645/BW25113_RS26145 RHS repeat protein/hypothetical protein
* * ÷ NZ_CP009273 1524422 1524470 49 2 [1] [0] 2 BW25113_RS26145 hypothetical protein
* * ÷ NZ_CP009273 1524814 1524848 35 2 [0] [1] 2 [BW25113_RS07655] [BW25113_RS07655]
* * ÷ NZ_CP009273 1540377 1540624 248 2 [1] [1] 2 [yddG] [yddG]
* * ÷ NZ_CP009273 1543132 1543163 32 2 [1] [1] 2 fdnG formate dehydrogenase‑N subunit alpha
* * ÷ NZ_CP009273 1566560 1566563 4 2 [0] [0] 2 gadB/pqqL glutamate decarboxylase/insulinase family protein
* * ÷ NZ_CP009273 1588750 1588771 22 2 [1] [1] 2 BW25113_RS07915 ECSE_1600 family autotransporter
* * ÷ NZ_CP009273 1591458 1591638 181 4 [1] [1] 2 BW25113_RS07915 ECSE_1600 family autotransporter
* * ÷ NZ_CP009273 1620106 1620115 10 2 [1] [1] 2 dcp peptidyl‑dipeptidase Dcp
* * ÷ NZ_CP009273 1627557 1627843–1627567 11–287 2 [1] [1] 2 [ydfK] [ydfK]
* * ÷ NZ_CP009273 1630721 1630757 37 2 [1] [1] 2 nohA/ynfO DNA‑packaging protein/YlcI/YnfO family protein
* * ÷ NZ_CP009273 1640766 1640830 65 3 [1] [0] 2 flxA FlxA‑like family protein
* * ÷ NZ_CP009273 1643672 1643700 29 2 [1] [1] 2 BW25113_RS08235/dicB hypothetical protein/cell division inhibition protein DicB
* * ÷ NZ_CP009273 1648568 1648594 27 3 [1] [1] 2 rspA starvation‑sensing protein RspA
* * ÷ NZ_CP009273 1648757 1648778 22 2 [1] [1] 2 rspA starvation‑sensing protein RspA
* * ÷ NZ_CP009273 1659047 1659060 14 2 [1] [1] 2 dmsD Tat proofreading chaperone DmsD
* * ÷ NZ_CP009273 1683466 1683471 6 2 [0] [0] 2 manA mannose‑6‑phosphate isomerase
* * ÷ NZ_CP009273 1729089 1729134 46 4 [1] [1] 3 mepH peptidoglycan DD‑endopeptidase MepH
* * ÷ NZ_CP009273 1748968 1749044 77 2 [1] [1] 2 ydhY/fumD ferredoxin‑like protein/fumarate hydratase FumD
* * ÷ NZ_CP009273 1754602 1754610 9 2 [0] [1] 2 sufS cysteine desulfurase SufS
* * ÷ NZ_CP009273 1756052 1756061 10 2 [0] [0] 2 sufC Fe‑S cluster assembly ATPase SufC
* * ÷ NZ_CP009273 1772611 1772669 59 2 [1] [1] 2 [ydiP] [ydiP]
* * ÷ NZ_CP009273 1778276 1778283 8 3 [0] [0] 2 fadK medium‑chain fatty‑acid‑‑CoA ligase
* * ÷ NZ_CP009273 1807507 1807567 61 2 [1] [0] 2 BW25113_RS09050/cedA hypothetical protein/cell division activator CedA
* * ÷ NZ_CP009273 1822122 1822213 92 2 [1] [1] 2 astB N‑succinylarginine dihydrolase
* * ÷ NZ_CP009273 1828178 1828218 41 2 [1] [1] 2 ydjX TVP38/TMEM64 family protein
* * ÷ NZ_CP009273 1871376 1871453 78 3 [1] [1] 3 yeaO DUF488 domain‑containing protein
* * ÷ NZ_CP009273 1882157 1882213 57 2 [1] [1] 2 rnd ribonuclease D
* * ÷ NZ_CP009273 1926685 1926692 8 2 [0] [1] 2 kdgA bifunctional 4‑hydroxy‑2‑oxoglutarate aldolase/2‑dehydro‑3‑deoxy‑phosphogluconate aldolase
* * ÷ NZ_CP009273 1927561 1927572 12 2 [1] [1] 3 edd phosphogluconate dehydratase
* * ÷ NZ_CP009273 1962195 1962205 11 4 [0] [1] 2 cheB protein‑glutamate methylesterase/protein glutamine deamidase
* * ÷ NZ_CP009273 2042196 2042250 55 2 [0] [1] 2 yeeJ inverse autotransporter adhesin YeeJ
* * ÷ NZ_CP009273 2047096 2047111 16 3 [0] [1] 2 BW25113_RS10340/shiA glycosyltransferase family 9 protein/shikimate transporter
* * ÷ NZ_CP009273 2083875 2083959 85 3 [0] [1] 5 hisG ATP phosphoribosyltransferase
* * ÷ NZ_CP009273 2099664 2099698 35 2 [1] [0] 2 wzy O16 family O‑antigen polymerase
* * ÷ NZ_CP009273 2111913 2111996 84 2 [1] [1] 2 wcaK/wzxC colanic acid biosynthesis pyruvyl transferase WcaK/colanic acid undecaprenyl disphosphate flippase WzxC
* * ÷ NZ_CP009273 2112516 2112528 13 2 [1] [1] 3 wzxC colanic acid undecaprenyl disphosphate flippase WzxC
* * ÷ NZ_CP009273 2141171 2141188 18 2 [1] [1] 2 yegD molecular chaperone
* * ÷ NZ_CP009273 2142639 2142656 18 2 [1] [0] 2 yegI protein kinase YegI
* * ÷ NZ_CP009273 2152896 2152946 51 2 [1] [1] 2 mdtC multidrug efflux RND transporter permease subunit MdtC
* * ÷ NZ_CP009273 2185819 2185878 60 2 [1] [1] 2 yehD/yehE fimbrial protein/YehE family protein
* * ÷ NZ_CP009273 2198308 2198381 74 2 [1] [1] 2 yehL MoxR family ATPase
* * ÷ NZ_CP009273 2215438 2215456 19 3 [1] [0] 4 bglX beta‑glucosidase BglX
* * ÷ NZ_CP009273 2336107 2336120 14 2 [1] [1] 2 yfaL AIDA‑I family autotransporter adhesin YfaL/EhaC
* * ÷ NZ_CP009273 2370441 2370471 31 2 [1] [1] 2 menH 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
* * ÷ NZ_CP009273 2377196 2377260 65 4 [1] [1] 2 elaD deubiquitinase ElaD
* * ÷ NZ_CP009273 2378772 2378783 12 2 [0] [1] 2 yfbK VWA domain‑containing protein
* * ÷ NZ_CP009273 2379166 2379206 41 2 [1] [1] 2 [yfbK] [yfbK]
* * ÷ NZ_CP009273 2451711 2451718 8 2 [0] [0] 2 fadJ fatty acid oxidation complex subunit alpha FadJ
* * ÷ NZ_CP009273 2488082 2488106 25 2 [1] [0] 4 yfdX/ypdI YfdX family protein/colanic acid biosynthesis lipoprotein YpdI
* * ÷ NZ_CP009273 2505473 2505482 10 2 [0] [1] 2 mntH Nramp family divalent metal transporter
* * ÷ NZ_CP009273 2616219 2616235 17 2 [1] [1] 3 [purN] [purN]
* * ÷ NZ_CP009273 2623049 2623100 52 2 [1] [1] 2 yfgG/yfgH YfgG family protein/glycine zipper 2TM domain‑containing protein
* * ÷ NZ_CP009273 2735108 2735123 16 3 [1] [0] 2 yfiL/yfiR DUF2799 domain‑containing protein/YfiR family protein
* * ÷ NZ_CP009273 2768213 2768263 51 3 [1] [1] 2 BW25113_RS13770 hypothetical protein
* * ÷ NZ_CP009273 3303745 3303792 48 2 [1] [1] 4 pnp polyribonucleotide nucleotidyltransferase
* * ÷ NZ_CP009273 3448796 3448866 71 2 [1] [1] 3 gspA/gspC type II secretion system protein GspA/type II secretion system protein GspC
* * ÷ NZ_CP009273 3471307 3471319 13 2 [1] [1] 2 slyD peptidylprolyl isomerase
* * ÷ NZ_CP009273 3581662 3581727 66 3 [1] [1] 2 ugpC sn‑glycerol 3‑phosphate ABC transporter ATP binding protein UgpC
* * ÷ NZ_CP009273 3763096 3763106 11 2 [0] [1] 2 BW25113_RS26240 Imm57 family immunity protein
* * ÷ NZ_CP009273 3792523 3792557 35 2 [0] [1] 3 waaU glycosyltransferase family 9 protein
* * ÷ NZ_CP009273 3798225 3798236 12 2 [1] [1] 2 rfaS LPS core biosynthesis protein RfaS
* * ÷ NZ_CP009273 3830115 3830150 36 2 [1] [0] 3 BW25113_RS26245/setC virulence RhuM family protein/sugar efflux transporter
* * ÷ NZ_CP009273 3875350 3875370 21 2 [1] [1] 2 dnaN DNA polymerase III subunit beta
* * ÷ NZ_CP009273 4294368 4294379 12 2 [1] [1] 2 ytcA/yjcS YtcA family lipoprotein/alkyl sulfatase YjcS

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)23 (1.900) 17/170 0.0 46.9% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 26 (2.150)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 19727511 (0.080)16 (1.320) 12/170 0.2 94.1% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 4532499 =4 (0.330)4 (0.370) 4/152 0.8 23.8% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 22 (2.040)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA