mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 231 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 433 | 0 | T | G | 56.8% | 40.2 / 161.5 | 118 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| * | REL606 | 2,437,406 | 0 | G | T | 51.1% | 14.8 / 60.1 | 45 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
| * | REL606 | 2,515,909 | 0 | A | C | 46.9% | 80.8 / 54.9 | 81 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| * | REL606 | 1,747,903 | 0 | G | T | 46.6% | 515.6 / inf | 605 | A8S (GCA→TCA) | ydiL | hypothetical protein |
| * | REL606 | 4,180,249 | 0 | T | G | 46.6% | 140.7 / 52.5 | 117 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| * | REL606 | 2,213,003 | 0 | A | C | 44.2% | 141.9 / 26.1 | 104 | V184G (GTG→GGG) | fruK | 1‑phosphofructokinase |
| * | REL606 | 1,854,401 | 0 | A | C | 43.3% | 113.9 / 58.3 | 104 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| * | REL606 | 3,774,914 | 0 | C | G | 42.0% | 280.7 / 218.9 | 219 | A20P (GCC→CCC) | yicJ | predicted transporter |
| * | REL606 | 1,118,209 | 0 | G | T | 41.3% | 58.0 / 76.6 | 75 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
| * | REL606 | 895,856 | 0 | G | C | 41.3% | 52.6 / 38.2 | 46 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
| * | REL606 | 931,314 | 0 | T | C | 39.4% | 112.0 / 50.0 | 104 | E84G (GAG→GGG) | ybjE | predicted transporter |
| * | REL606 | 3,378,885 | 0 | T | G | 37.6% | 403.7 / 65.6 | 213 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
| * | REL606 | 2,376,348 | 0 | T | G | 37.1% | 328.0 / 23.1 | 170 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| * | REL606 | 4,392,556 | 0 | C | T | 36.8% | 250.1 / 121.8 | 191 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
| * | REL606 | 3,472,700 | 0 | C | G | 36.6% | 106.6 / 86.8 | 101 | A290G (GCG→GGG) | yhgA | predicted transposase |
| * | REL606 | 2,676,636 | 0 | T | G | 36.1% | 450.8 / 91.0 | 253 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
| * | REL606 | 1,759,444 | 0 | A | C | 35.7% | 327.4 / 29.7 | 154 | H261P (CAC→CCC) | ydiS | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| * | REL606 | 1,485,450 | 0 | A | G | 35.7% | 421.3 / 156.9 | 241 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
| * | REL606 | 4,111,429 | 0 | T | G | 35.2% | 130.6 / 14.8 | 71 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
| * | REL606 | 720,464 | 0 | C | G | 35.0% | 23.8 / 10.2 | 20 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
| Marginal new junction evidence (lowest skew 10 of 57 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 379585 | 457 (1.690) | 207 (0.760) | 10/150 | 17.6 | 28.6% | intergenic (+110/+135) | yajF/araJ | fructokinase/predicted transporter |
| ? | REL606 | = 2583282 | 578 (2.130) | coding (741/741 nt) | yfhQ | predicted methyltransferase | |||||
| * | ? | REL606 | = 715842 | 249 (0.920) | 8 (0.030) | 7/134 | 18.2 | 3.7% | coding (449/570 nt) | ybfC | hypothetical protein |
| ? | REL606 | = 715985 | 189 (0.780) | intergenic (+22/‑174) | ybfC/ECB_00662 | hypothetical protein/conserved hypothetical protein | |||||
| * | ? | REL606 | 2884198 = | 240 (0.890) | 6 (0.020) | 6/142 | 19.4 | 2.5% | coding (304/552 nt) | ygeQ | hypothetical protein |
| ? | REL606 | 2884392 = | 243 (0.950) | coding (110/552 nt) | ygeQ | hypothetical protein | |||||
| * | ? | REL606 | = 4486967 | 287 (1.060) | 6 (0.030) | 4/128 | 19.8 | 2.1% | coding (1018/1218 nt) | yjhB | predicted transporter |
| ? | REL606 | = 4487104 | 306 (1.330) | coding (1155/1218 nt) | yjhB | predicted transporter | |||||
| * | ? | REL606 | 1518088 = | 300 (1.110) | 7 (0.030) | 5/140 | 19.9 | 2.4% | coding (124/1389 nt) | narU | nitrate/nitrite transporter |
| ? | REL606 | 1518282 = | 286 (1.130) | intergenic (‑71/+253) | narU/yddJ | nitrate/nitrite transporter/hypothetical protein | |||||
| * | ? | REL606 | = 799331 | 340 (1.250) | 6 (0.020) | 5/140 | 19.9 | 1.9% | intergenic (‑56/+728) | ECB_00739/ybhB | hypothetical protein/predicted kinase inhibitor |
| ? | REL606 | = 799578 | 306 (1.210) | intergenic (‑303/+481) | ECB_00739/ybhB | hypothetical protein/predicted kinase inhibitor | |||||
| * | ? | REL606 | 718423 = | 30 (0.110) | 6 (0.030) | 4/132 | 20.1 | 7.2% | pseudogene (427/858 nt) | yhhI | predicted transposase; b3484_1 |
| ? | REL606 | 3556027 = | 128 (0.540) | coding (962/1137 nt) | yhhI | predicted transposase | |||||
| * | ? | REL606 | 718423 = | 30 (0.110) | 6 (0.030) | 4/132 | 20.1 | 3.7% | pseudogene (427/858 nt) | yhhI | predicted transposase; b3484_1 |
| ? | REL606 | 718679 = | 290 (1.220) | pseudogene (683/858 nt) | yhhI | predicted transposase; b3484_1 | |||||
| * | ? | REL606 | = 568984 | 168 (0.620) | 6 (0.030) | 4/132 | 20.1 | 6.3% | coding (953/1137 nt) | yhhI | predicted transposase |
| ? | REL606 | 718432 = | 30 (0.130) | pseudogene (436/858 nt) | yhhI | predicted transposase; b3484_1 | |||||
| * | ? | REL606 | 3512999 = | 329 (1.210) | 4 (0.020) | 4/136 | 20.4 | 1.3% | coding (409/489 nt) | yhhY | predicted acetyltransferase |
| ? | REL606 | 3513238 = | 320 (1.300) | intergenic (+159/‑78) | yhhY/yhhZ | predicted acetyltransferase/hypothetical protein | |||||