mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Marginal read alignment evidence (highest frequency 20 of 257 shown, sorted by frequency from high to low) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,735,608 | 0 | G | C | 56.7% | 10.4 / 44.2 | 30 | A409P (GCC→CCC) | ascF | fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component |
| * | REL606 | 433 | 0 | T | G | 56.2% | 21.0 / 109.1 | 89 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
| * | REL606 | 2,515,909 | 0 | A | C | 51.2% | 96.6 / 135.8 | 80 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
| * | REL606 | 4,180,249 | 0 | T | G | 46.2% | 110.6 / 31.7 | 80 | G24G (GGT→GGG) | yjaH | hypothetical protein |
| * | REL606 | 476,746 | 0 | T | G | 45.7% | 122.7 / 33.4 | 105 | Q6H (CAA→CAC) | fsr | predicted fosmidomycin efflux system |
| * | REL606 | 1,798,750 | 0 | A | C | 45.5% | 265.9 / 89.9 | 167 | G45G (GGT→GGG) | celA | N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS |
| * | REL606 | 895,856 | 0 | G | C | 43.9% | 44.2 / 36.0 | 41 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
| * | REL606 | 1,854,401 | 0 | A | C | 43.5% | 60.4 / 30.7 | 62 | K88N (AAA→AAC) | yeaO | hypothetical protein |
| * | REL606 | 720,466 | 0 | G | C | 43.5% | 12.8 / 13.9 | 23 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
| * | REL606 | 3,774,914 | 0 | C | G | 43.0% | 178.5 / 139.2 | 142 | A20P (GCC→CCC) | yicJ | predicted transporter |
| * | REL606 | 2,376,348 | 0 | T | G | 41.8% | 208.1 / 30.5 | 135 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
| * | REL606 | 2,395,686 | 0 | T | G | 39.0% | 285.3 / 37.8 | 154 | L105F (TTA→TTC) | yfcS | predicted periplasmic pilus chaperone |
| * | REL606 | 68,560 | 0 | T | G | 38.4% | 226.7 / 29.2 | 125 | T9P (ACC→CCC) | polB | DNA polymerase II |
| * | REL606 | 1,759,444 | 0 | A | C | 38.0% | 298.7 / 26.0 | 142 | H261P (CAC→CCC) | ydiS | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| * | REL606 | 3,096,354 | 0 | A | C | 38.0% | 207.9 / 72.4 | 137 | V163G (GTG→GGG) | sufI | repressor protein for FtsI |
| * | REL606 | 2,144,295 | 0 | A | C | 37.7% | 361.0 / 78.8 | 183 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
| * | REL606 | 414,056 | 0 | T | G | 37.7% | 273.2 / 41.4 | 138 | T345P (ACC→CCC) | yajR | predicted transporter |
| * | REL606 | 2,304,407 | 0 | C | A | 37.1% | 97.3 / 77.4 | 70 | Q266K (CAA→AAA) | yfaD | hypothetical protein |
| * | REL606 | 2,676,636 | 0 | T | G | 37.0% | 414.8 / 43.5 | 189 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
| * | REL606 | 4,320,225 | 0 | T | G | 36.6% | 254.5 / 103.2 | 153 | T40P (ACC→CCC) | melR | DNA‑binding transcriptional dual regulator |
| Marginal new junction evidence (lowest skew 10 of 14 shown) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | = 4326090 | 437 (1.970) | 5 (0.030) | 5/132 | 16.6 | 1.3% | intergenic (‑49/+29) | fumB/dcuB | anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate antiporter |
| ? | REL606 | = 4326100 | 385 (1.980) | intergenic (‑59/+19) | fumB/dcuB | anaerobic class I fumarate hydratase (fumarase B)/C4‑dicarboxylate antiporter | |||||
| * | ? | REL606 | = 1941260 | 186 (0.840) | 9 (0.050) | 4/122 | 16.6 | 5.8% | intergenic (+30/+90) | yecT/flhE | hypothetical protein/hypothetical protein |
| ? | REL606 | = 1941278 | 144 (0.800) | intergenic (+48/+72) | yecT/flhE | hypothetical protein/hypothetical protein | |||||
| * | ? | REL606 | 402458 = | 208 (0.940) | 3 (0.020) | 3/118 | 17.2 | 1.8% | coding (582/1104 nt) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
| ? | REL606 | = 3276096 | 161 (0.930) | coding (587/978 nt) | yrbG | predicted calcium/sodium:proton antiporter | |||||
| * | ? | REL606 | 330032 = | 318 (1.440) | 61 (0.280) +C |
5/148 | 17.6 | 21.1% | intergenic (+22/+315) | codA/cynR | cytosine deaminase/DNA‑binding transcriptional dual regulator |
| ? | REL606 | = 330430 | 143 (0.650) | coding (817/900 nt) | cynR | DNA‑binding transcriptional dual regulator | |||||
| * | ? | REL606 | 79645 = | 232 (1.050) | 4 (0.020) | 4/146 | 18.2 | 1.7% | coding (459/1656 nt) | yabN | DNA‑binding transcriptional regulator |
| ? | REL606 | 79647 = | 227 (1.050) | coding (457/1656 nt) | yabN | DNA‑binding transcriptional regulator | |||||
| * | ? | REL606 | 4419506 = | 213 (0.960) | 3 (0.020) | 3/134 | 18.3 | 1.4% | intergenic (+13/+110) | ytfH/cpdB | predicted transcriptional regulator/bifunctional 2',3'‑cyclic nucleotide 2'‑phosphodiesterase/3'‑nucleotidase periplasmic precursor protein |
| ? | REL606 | = 4621373 | 221 (1.120) | intergenic (+74/+11) | trpR/yjjX | Trp operon repressor/NTPase | |||||
| * | ? | REL606 | = 461982 | 191 (0.860) | 4 (0.020) | 3/134 | 18.3 | 2.2% | intergenic (+17/+195) | kefA/ybaM | fused conserved protein/hypothetical protein |
| ? | REL606 | = 461987 | 180 (0.910) | intergenic (+22/+190) | kefA/ybaM | fused conserved protein/hypothetical protein | |||||
| * | ? | REL606 | 419059 = | 244 (1.100) | 6 (0.030) | 3/136 | 18.4 | 2.8% | coding (305/615 nt) | cyoC | cytochrome o ubiquinol oxidase subunit III |
| ? | REL606 | 419079 = | 197 (0.980) | coding (285/615 nt) | cyoC | cytochrome o ubiquinol oxidase subunit III | |||||
| * | ? | REL606 | 1181703 = | 179 (0.810) | 4 (0.020) | 4/150 | 18.5 | 1.9% | coding (1042/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
| ? | REL606 | 1663278 = | 232 (1.050) | coding (506/1404 nt) | fumC | fumarate hydratase | |||||
| * | ? | REL606 | 3032251 = | 215 (0.970) | 4 (0.020) | 3/138 | 18.6 | 1.9% | coding (522/564 nt) | ECB_02832 | hypothetical type II secretion protein GspJ |
| ? | REL606 | 3032270 = | 206 (1.010) | coding (503/564 nt) | ECB_02832 | hypothetical type II secretion protein GspJ | |||||