breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 248 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 720,466 | 0 | G | C | 55.0% | ‑4.0 / 22.7 | 20 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 430,318 | 0 | T | C | 53.3% | ‑5.9 / 11.5 | 15 | D157G (GAC→GGC) | insL‑2 | putative transposase insL for insertion sequence IS186 |
* | REL606 | 2,735,608 | 0 | G | C | 52.9% | 6.3 / 21.6 | 17 | A409P (GCC→CCC) | ascF | fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component |
* | REL606 | 2,515,909 | 0 | A | C | 52.7% | 72.2 / 118.9 | 74 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 433 | 0 | T | G | 51.2% | 56.4 / 122.4 | 82 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 4,180,249 | 0 | T | G | 50.5% | 98.4 / 144.1 | 95 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 2,007,028 | 0 | C | A | 44.4% | 30.6 / 28.8 | 27 | intergenic (‑152/+503) | insC/ECB_01906 | insertion sequence 2 OrfA protein; b1997/conserved hypothetical protein |
* | REL606 | 414,056 | 0 | T | G | 42.3% | 164.3 / 47.1 | 104 | T345P (ACC→CCC) | yajR | predicted transporter |
* | REL606 | 4,169,225 | 0 | T | G | 40.3% | 436.1 / 30.2 | 228 | intergenic (+41/‑172) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 2,676,636 | 0 | T | G | 38.9% | 268.7 / 62.1 | 157 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
* | REL606 | 1,118,209 | 0 | G | T | 38.8% | 48.5 / 62.6 | 67 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
* | REL606 | 1,854,401 | 0 | A | C | 38.5% | 136.2 / 35.1 | 91 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,437,406 | 0 | G | T | 37.5% | 57.0 / 13.2 | 32 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 2,144,295 | 0 | A | C | 35.8% | 324.3 / 73.1 | 162 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
* | REL606 | 3,984,462 | 0 | T | G | 35.8% | 231.9 / 15.4 | 109 | T143P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 1,519,907 | 0 | C | G | 35.6% | 280.9 / 123.6 | 146 | S91S (TCG→TCC) | yddL | predicted lipoprotein |
* | REL606 | 3,305,660 | 0 | A | C | 35.6% | 418.1 / 19.6 | 177 | intergenic (‑342/+53) | sspA/rpsI | stringent starvation protein A/30S ribosomal protein S9 |
* | REL606 | 390,957 | 0 | A | C | 35.2% | 352.3 / 56.3 | 160 | H7P (CAC→CCC) | malZ | maltodextrin glucosidase |
* | REL606 | 4,392,556 | 0 | C | T | 34.9% | 177.7 / 67.9 | 126 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 452,666 | 0 | A | C | 34.9% | 462.1 / 37.0 | 212 | V37G (GTA→GGA) | ybaJ | hypothetical protein |
Marginal new junction evidence (lowest skew 10 of 15 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 797805 = | 189 (0.960) | 13 (0.070) | 6/140 | 14.8 | 6.0% | coding (1051/1338 nt) | ECB_00738 | hypothetical protein |
? | REL606 | 798250 = | 229 (1.250) | coding (1026/1146 nt) | ECB_00739 | hypothetical protein | |||||
* | ? | REL606 | 3683510 = | 178 (0.910) | 6 (0.040) +TAGCAGCGCTGCTA |
4/122 | 15.0 | 4.0% | coding (1201/1401 nt) | ECB_03437 | putative permease |
? | REL606 | 3683510 = | 178 (0.910) | coding (1201/1401 nt) | ECB_03437 | putative permease | |||||
* | ? | REL606 | 2680076 = | 230 (1.170) | 8 (0.040) | 5/148 | 15.9 | 3.6% | coding (1148/1557 nt) | ECB_02510 | Recombinase |
? | REL606 | 2680568 = | 202 (1.040) | coding (94/897 nt) | ECB_02511 | ParB family protein | |||||
* | ? | REL606 | = 2041060 | 177 (0.900) | 4 (0.020) | 4/136 | 15.9 | 2.7% | coding (324/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | = 2041460 | 132 (0.740) | coding (1361/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 1143523 = | 181 (0.920) | 4 (0.020) | 4/136 | 15.9 | 2.3% | coding (1113/1536 nt) | mviN | predicted inner membrane protein |
? | REL606 | 1143547 = | 179 (1.010) | coding (1137/1536 nt) | mviN | predicted inner membrane protein | |||||
* | ? | REL606 | = 1219187 | 174 (0.890) | 7 (0.040) | 4/138 | 16.0 | 4.2% | coding (44/219 nt) | ymgF | hypothetical protein |
? | REL606 | = 1219601 | 158 (0.880) | intergenic (+239/‑161) | ymgF/ycgH | hypothetical protein/predicted protein; b4491 | |||||
* | ? | REL606 | = 819771 | 162 (0.830) | 6 (0.030) +CTGCTA |
4/138 | 16.0 | 3.7% | coding (107/1134 nt) | ybhS | predicted transporter subunit: membrane component of ABC superfamily |
? | REL606 | 3683510 = | 178 (0.910) | coding (1201/1401 nt) | ECB_03437 | putative permease | |||||
* | ? | REL606 | = 819771 | 162 (0.830) | 6 (0.030) | 4/146 | 16.5 | 3.7% | coding (107/1134 nt) | ybhS | predicted transporter subunit: membrane component of ABC superfamily |
? | REL606 | = 819769 | 154 (0.810) | coding (109/1134 nt) | ybhS | predicted transporter subunit: membrane component of ABC superfamily | |||||
* | ? | REL606 | 1144177 = | 258 (1.310) | 5 (0.030) | 3/134 | 16.6 | 1.9% | coding (226/417 nt) | flgN | export chaperone for FlgK and FlgL |
? | REL606 | 1144199 = | 277 (1.580) | coding (204/417 nt) | flgN | export chaperone for FlgK and FlgL | |||||
* | ? | REL606 | 3021237 = | 175 (0.890) | 5 (0.030) | 3/142 | 17.0 | 2.2% | coding (247/933 nt) | ECB_02820 | hypothetical protein |
? | REL606 | 3021461 = | 278 (1.500) | coding (23/933 nt) | ECB_02820 | hypothetical protein |