breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 248 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606720,4660GC55.0% ‑4.0 / 22.7 20A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL606430,3180TC53.3% ‑5.9 / 11.5 15D157G (GAC→GGC) insL‑2putative transposase insL for insertion sequence IS186
*REL6062,735,6080GC52.9% 6.3 / 21.6 17A409P (GCC→CCC) ascFfused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
*REL6062,515,9090AC52.7% 72.2 / 118.9 74V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6064330TG51.2% 56.4 / 122.4 82V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6064,180,2490TG50.5% 98.4 / 144.1 95G24G (GGT→GGGyjaHhypothetical protein
*REL6062,007,0280CA44.4% 30.6 / 28.8 27intergenic (‑152/+503)insC/ECB_01906insertion sequence 2 OrfA protein; b1997/conserved hypothetical protein
*REL606414,0560TG42.3% 164.3 / 47.1 104T345P (ACC→CCC) yajRpredicted transporter
*REL6064,169,2250TG40.3% 436.1 / 30.2 228intergenic (+41/‑172)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6062,676,6360TG38.9% 268.7 / 62.1 157V149G (GTG→GGG) smpBSsrA‑binding protein
*REL6061,118,2090GT38.8% 48.5 / 62.6 67intergenic (‑444/‑311)csgD/csgBDNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex
*REL6061,854,4010AC38.5% 136.2 / 35.1 91K88N (AAA→AACyeaOhypothetical protein
*REL6062,437,4060GT37.5% 57.0 / 13.2 32D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL6062,144,2950AC35.8% 324.3 / 73.1 162G357G (GGT→GGGmrpantiporter inner membrane protein
*REL6063,984,4620TG35.8% 231.9 / 15.4 109T143P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6061,519,9070CG35.6% 280.9 / 123.6 146S91S (TCG→TCCyddLpredicted lipoprotein
*REL6063,305,6600AC35.6% 418.1 / 19.6 177intergenic (‑342/+53)sspA/rpsIstringent starvation protein A/30S ribosomal protein S9
*REL606390,9570AC35.2% 352.3 / 56.3 160H7P (CAC→CCC) malZmaltodextrin glucosidase
*REL6064,392,5560CT34.9% 177.7 / 67.9 126L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL606452,6660AC34.9% 462.1 / 37.0 212V37G (GTA→GGA) ybaJhypothetical protein

Marginal new junction evidence (lowest skew 10 of 15 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 797805 =189 (0.960)13 (0.070) 6/140 14.8 6.0% coding (1051/1338 nt) ECB_00738 hypothetical protein
?REL606 798250 = 229 (1.250)coding (1026/1146 nt) ECB_00739 hypothetical protein
* ? REL606 3683510 =178 (0.910)6 (0.040)
+TAGCAGCGCTGCTA
4/122 15.0 4.0% coding (1201/1401 nt) ECB_03437 putative permease
?REL606 3683510 = 178 (0.910)coding (1201/1401 nt) ECB_03437 putative permease
* ? REL606 2680076 =230 (1.170)8 (0.040) 5/148 15.9 3.6% coding (1148/1557 nt) ECB_02510 Recombinase
?REL606 2680568 = 202 (1.040)coding (94/897 nt) ECB_02511 ParB family protein
* ? REL606 = 2041060177 (0.900)4 (0.020) 4/136 15.9 2.7% coding (324/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 = 2041460 132 (0.740)coding (1361/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 1143523 =181 (0.920)4 (0.020) 4/136 15.9 2.3% coding (1113/1536 nt) mviN predicted inner membrane protein
?REL606 1143547 = 179 (1.010)coding (1137/1536 nt) mviN predicted inner membrane protein
* ? REL606 = 1219187174 (0.890)7 (0.040) 4/138 16.0 4.2% coding (44/219 nt) ymgF hypothetical protein
?REL606 = 1219601 158 (0.880)intergenic (+239/‑161) ymgF/ycgH hypothetical protein/predicted protein; b4491
* ? REL606 = 819771162 (0.830)6 (0.030)
+CTGCTA
4/138 16.0 3.7% coding (107/1134 nt) ybhS predicted transporter subunit: membrane component of ABC superfamily
?REL606 3683510 = 178 (0.910)coding (1201/1401 nt) ECB_03437 putative permease
* ? REL606 = 819771162 (0.830)6 (0.030) 4/146 16.5 3.7% coding (107/1134 nt) ybhS predicted transporter subunit: membrane component of ABC superfamily
?REL606 = 819769 154 (0.810)coding (109/1134 nt) ybhS predicted transporter subunit: membrane component of ABC superfamily
* ? REL606 1144177 =258 (1.310)5 (0.030) 3/134 16.6 1.9% coding (226/417 nt) flgN export chaperone for FlgK and FlgL
?REL606 1144199 = 277 (1.580)coding (204/417 nt) flgN export chaperone for FlgK and FlgL
* ? REL606 3021237 =175 (0.890)5 (0.030) 3/142 17.0 2.2% coding (247/933 nt) ECB_02820 hypothetical protein
?REL606 3021461 = 278 (1.500)coding (23/933 nt) ECB_02820 hypothetical protein