breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 32 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH1936,1690T.35.1% 259.1 / 155.6 118intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH13,448,5260AC30.3% 799.0 / 152.4 476intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1936,2930T.29.0% 346.9 / 154.1 144intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,906,8320CA27.9% 178.3 / 15.8 116intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1613,7700CA27.7% 274.6 / 23.8 239intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,5770TG26.9% 341.7 / 74.4 310R661R (CGA→CGCrsxCelectron transporter RsxC
*DH12,169,5480GT26.7% 602.5 / 85.3 319P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH11,065,6180AG26.4% 466.8 / 188.3 298intergenic (+53/‑10)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH12,169,7200CG26.3% 208.2 / 59.6 205E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH13,448,5230AC25.9% 1002.5 / 98.3 476intergenic (‑83/+73)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH1936,2781.T25.3% 211.8 / 120.4 91intergenic (‑12/+22)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,326,2870GT25.0% 1309.3 / 144.2 617intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH11,065,6050AT24.7% 434.8 / 120.2 256intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH1613,5970GC24.3% 480.1 / 59.9 309intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,591,5130TG24.2% 286.9 / 48.6 297D86A (GAC→GCC) ECDH1_RS12815hypothetical protein
*DH13,447,4030AC23.9% 849.8 / 88.1 435G346G (GGT→GGGmalZalpha‑glycosidase
*DH1613,6130TG23.8% 243.8 / 34.8 244intergenic (+474/+549)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH13,906,8370CG23.4% 203.5 / 17.0 122intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1936,2970AG23.0% 467.1 / 135.5 215intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH13,184,3180TA22.8% 406.5 / 68.0 206noncoding (30/75 nt)ECDH1_RS15785tRNA‑Gln

Marginal new junction evidence (lowest skew 10 of 52 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 2668875451 (0.770)131 (0.250) 102/268 14.0 24.4% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 = 2670672 407 (0.780)coding (306/789 nt) stfP integrase
* ? DH1 2668875 =401 (0.760)127 (0.240) 97/268 15.3 24.2% pseudogene (20/345 nt) pinE hypothetical protein
?DH1 2670674 = NA (NA)noncoding (1/242 nt) repeat_region repeat region
* ? DH1 = 3184062552 (0.940)98 (0.240)
+45 bp
38/210 29.3 26.3% noncoding (77/77 nt) ECDH1_RS15770 tRNA‑Met
?DH1 3184613 = 234 (0.400)intergenic (+36/‑2) ECDH1_RS15795/ECDH1_RS15800 tRNA‑Gln/tRNA‑TERM
* ? DH1 = 1519281391 (0.670)73 (0.140)
+AACCTTCTAATT
51/276 32.1 16.9% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 391 (0.670)coding (787/900 nt) yfaD transposase
* ? DH1 1355966 =248 (0.420)99 (0.170)
+CTTC
55/292 32.3 25.1% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 359 (0.610)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1519086406 (0.690)45 (0.110) 35/220 32.4 14.1% coding (71/186 nt) ECDH1_RS07485 hypothetical protein
?DH1 1519282 = 249 (0.580)coding (787/900 nt) yfaD transposase
* ? DH1 3183985 =597 (1.020)107 (0.200)
+GATTGTTTCTTC
48/276 33.5 22.4% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 207 (0.350)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 1355843 =152 (0.260)141 (0.240)
+C
54/298 33.5 35.7% intergenic (‑44/+3) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 359 (0.610)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 2590941543 (0.930)71 (0.140) 37/260 36.9 17.5% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 197 (0.390)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 = 1355505569 (0.970)61 (0.120) 35/260 38.0 13.2% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 306 (0.600)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys