Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F21 I0 R1
|
50 |
53.6 |
2034412 |
90.9% |
1849280 |
142.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
100% |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 100.0%
| 151.3
/ ‑6.7
| 52 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (27/23); total (28/24) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG > NC_000913/759110‑759389
|
ccAGGAGCCCTTCGACTGCGGCTCACTTGCCAGCGGCCAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:902115/143‑1 (MQ=255)
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:13066/143‑1 (MQ=255)
ggAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTCCATCAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa < 1:469420/143‑1 (MQ=255)
gcagaCCTGCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa < 1:111305/141‑1 (MQ=255)
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCAAcc > 2:165799/1‑142 (MQ=255)
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 2:277750/143‑1 (MQ=255)
cAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 1:813302/143‑1 (MQ=255)
gTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGGACCGAAGAAAAACTCTGGATCCAACAGCGTAt < 2:531275/143‑1 (MQ=255)
ctCGGTTCAGTGGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTAt < 1:264567/142‑1 (MQ=255)
gTTCATTTACCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGCTCCAACAGCGTAGCGAg > 2:998722/1‑143 (MQ=255)
ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGCTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTACCCAGt > 1:509768/1‑143 (MQ=255)
tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc > 1:769719/1‑143 (MQ=255)
aTTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg < 1:902601/143‑1 (MQ=255)
aTTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTACCGAGTCTg > 1:977489/1‑143 (MQ=255)
tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTcaag > 1:224042/1‑140 (MQ=255)
ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCGCATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTCTCGAGACTGGTcgc > 1:700682/1‑143 (MQ=255)
cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGATGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa > 2:495337/1‑143 (MQ=255)
cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCCCCGAAGAAACACTCTGGATCCAACAGCGTCTCGAGCCTAGGcgcgcgc > 2:474443/1‑142 (MQ=255)
cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCCCCGAAGAAAAACGCTGGATCCAACAGCGGATCGAGACTGGGCGCGCGa > 2:269930/1‑143 (MQ=255)
ggCAAAGAAACCATGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 1:689910/143‑1 (MQ=255)
gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTACCGCGTCTCGTCGCGCCAcc > 2:774774/1‑142 (MQ=255)
cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtt < 1:206131/143‑1 (MQ=255)
aGAAACCATGGAGCGCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCGACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCa < 2:105756/143‑1 (MQ=255)
aCCATGACACTCGGCGAGCTGCTGGAAGACCTCAAGAAAAGATACTGGGGCCCGCTTGGTGCCGCGTATATGCGCATTACCGGCACCGAGGATAGACTCTGGGGCCAACAGCACCTCGAGCGCGGTAGCGCGAATTTcgtgca > 2:219085/1‑138 (MQ=255)
tGAAGCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa < 2:236269/143‑1 (MQ=255)
gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGGGGATATGCACATTACCAGCACCGCACACCAACTCTGGTTCCAACAGCGTATCGAGTCTGGTCGCGCGACGTTCGATAGCGAAGTGAAAAAACGcttct > 1:94283/1‑143 (MQ=255)
gctgGAAGCCCTCAAGCAAACCTACTGCGGCCAGAATGGGGCCTTGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCGATAGCGAAGTGAAAAAACGcttct > 1:193918/1‑143 (MQ=255)
gCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccg > 2:856385/1‑143 (MQ=255)
cTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTg > 1:233680/1‑143 (MQ=255)
tACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGa > 1:770122/1‑143 (MQ=255)
tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCCTCTTAAGCGAACTGACCGCCGCTGACgg > 1:967908/1‑143 (MQ=255)
gCCCGATTGGTACCTCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGTATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAACAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 2:41361/143‑1 (MQ=255)
ccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGa < 2:830080/143‑1 (MQ=255)
ccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGa > 1:661406/1‑143 (MQ=255)
ttttGGTGCTGAGTCTATGCACATTACCAGCACCGAAGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc < 2:74126/141‑1 (MQ=255)
gATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc < 2:82955/143‑1 (MQ=255)
gggCCGAGGATATGCACATTACCCGCACCGACGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAc < 1:537729/141‑1 (MQ=255)
aGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 1:483805/143‑1 (MQ=255)
tataTGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcgc < 1:212817/143‑1 (MQ=255)
tGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCCATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGCTCTTGAACGCTACCTCGGCGCaaaa > 1:535148/1‑143 (MQ=255)
acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa < 1:738431/74‑1 (MQ=255)
acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa > 2:738431/1‑74 (MQ=255)
caTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc > 1:616308/1‑143 (MQ=255)
aTTAGCACCACCGAAGAAAAACTCTGGATCCACCAGCGTATCGAGTCTGGTCCCGCGCCTTTCAATAGCGTAGAGAAAAAACGCTTCTTAAGCGAACTGCCCGTCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc < 1:350335/143‑1 (MQ=255)
ccAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 2:509768/143‑1 (MQ=255)
ccAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 1:495337/143‑1 (MQ=255)
gaGCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGGCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg < 2:333282/142‑1 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCt > 2:698746/1‑111 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCt < 1:698746/111‑1 (MQ=255)
cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTGCCTGGCGCaa > 1:303150/1‑143 (MQ=255)
cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa > 1:826819/1‑143 (MQ=255)
aCCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGCAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaa > 2:651877/1‑143 (MQ=255)
aaaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAACTCCCTGGCGCAAAACGCTtc > 2:881405/1‑143 (MQ=255)
aaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAACGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGGAACGCACGTTACCTCGGCGCAAAATTCCCTGGCGCAAGACGCTTCTCg > 2:933840/1‑143 (MQ=255)
|
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG > NC_000913/759110‑759389
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
