Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F44 I1 R1	25	56.0	2153868	90.0%	1938481	142.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,250	G→T	R182L (CGC→CTC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,250	0	G	T	97.5%	104.9 / NA	41	R182L (CGC→CTC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/20); minor base C (0/1); total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG  >  NC_000913/759119‑759389

cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGCTCCacc                                                                                                                                  >  1:662045/1‑141 (MQ=255)

cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCGGAGTATATGCACATGACCAGCACGGAGGAAAAACTCTGGACCCagc                                                                                                                                  >  1:551950/1‑141 (MQ=255)

cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAAc                                                                                                                                  >  1:27601/1‑143 (MQ=255)

    aaCGTCGGTTCACGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCg                                                                                                                              <  2:312780/143‑1 (MQ=255)

     aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGt                                                                                                                             >  1:799284/1‑143 (MQ=255)

     aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGt                                                                                                                             <  2:97819/143‑1 (MQ=255)

     aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACAGCGGCACGATCGGCGCCGAGTATATGCACATACCCGTCACCGGAGGAAAACTCGGGATCCAACCGcct                                                                                                                             >  2:754903/1‑141 (MQ=255)

      cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTa                                                                                                                            >  1:272370/1‑143 (MQ=255)

      cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCGCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTAGATGCACATTACCAGCACCGAAGAAAAACTCCGAATCCAAGAGCggc                                                                                                                            >  2:1015396/1‑141 (MQ=255)

       gTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTAt                                                                                                                           <  1:520649/143‑1 (MQ=255)

        tagggtCATTTGCCGGCCGCAAAGAAATCATCAAACTCGGCGAGCTCCTGGAAGACCTCATGCAAACCTACAGCGGCCCGATTGGTGCCGAGTATATGCACATTACTAGCACCGAATAAAAACTCTGGATCCAACAGCGTATc                                                                                                                          <  2:815304/138‑1 (MQ=255)

                tttGCCAGCGGCAAAGAACCCATGAACCTCGGCGAGCTGCTGGAGGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg                                                                                                                  <  1:342475/143‑1 (MQ=255)

                  tGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTc                                                                                                                <  1:810692/143‑1 (MQ=255)

                     cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg                                                                                                             >  1:814688/1‑143 (MQ=255)

                       gCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcgcg                                                                                                           <  1:203356/143‑1 (MQ=255)

                                   ccATGAGACTCGGCGAGCCGCTGGAAGCCCTCAAGCAAAACTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                               <  1:983939/143‑1 (MQ=255)

                                        aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAaga                                                                                          >  1:224035/1‑143 (MQ=255)

                                            tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACCTTCAGTACCGAAGAGaag                                                                                      >  1:952533/1‑142 (MQ=255)

                                             cGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACCGCGGCCCGACTGGTGCCGCGCACATGCACATTACCCGCACCGAATAAAAACTCTGGATCCAACAACGAATTGATCATGGACGAGCGACTTTCAATAGCGAAGCGaaaa                                                                                     >  1:1031372/1‑143 (MQ=255)

                                                  aGCTGCTGTAAGCGCCCAAGCAAACCTACTGCCGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCCAAGAACAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc                                                                                <  2:44882/143‑1 (MQ=255)

                                                    ctgctgGAAGCCCTCAAGCAAACCTACTGCTGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt                                                                              <  1:628987/143‑1 (MQ=255)

                                                      gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttct                                                                            <  1:967105/143‑1 (MQ=255)

                                                       ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt                                                                           >  2:1008288/1‑143 (MQ=255)

                                                          gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATTCAACACCGGATCCAGTCTGGTCGCGCGACTTTCAATAGCGACGAGCAAAAACGCTTGTTAAg                                                                        >  2:644091/1‑143 (MQ=255)

                                                           aaGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc                                                                       <  2:439341/143‑1 (MQ=255)

                                                                cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGGAAAACAATGGAGACAAAAGAGCAGAAATTCCTTTCGCGCGACATTCCATAGCGAAGAAAAAAAACGCTTCTTAAGCGGACt                                                                  >  2:375488/1‑143 (MQ=255)

                                                                cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGCGAAAAAACGCTTCTTAAGCGAACt                                                                  >  1:425264/1‑143 (MQ=255)

                                                                   aagcaagcCTCCTGCGGCCGGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAACACCTCTGGATCCAACCGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc                                                               <  2:156818/143‑1 (MQ=255)

                                                                        aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCCATAGCGCAGAGAAACACCGCTTCTTAAGCGAACTGAccgccg                                                          >  2:702800/1‑143 (MQ=255)

                                                                             aCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa                                                     <  1:795849/143‑1 (MQ=255)

                                                                             aCTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa                                                     <  1:130952/143‑1 (MQ=255)

                                                                                   gCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt                                               <  2:799284/143‑1 (MQ=255)

                                                                                       gATTGGTGGCGAGTATAGGCACAGTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc                                           <  1:759757/143‑1 (MQ=255)

                                                                                           ggcgCCGAGTATATGCACATTACCAGCACCGAAGAAGAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa                                       <  1:1038932/140‑1 (MQ=255)

                                                                                               ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAACGCCGCTGAAGGTCTTGAACCTTACCTc                                   >  2:315821/1‑143 (MQ=255)

                                                                                               ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGCCGCGCGCCTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGGCCTGACCGCCGCTGAAGGTCCCGAACGTTACCTc                                   >  2:292969/1‑143 (MQ=255)

                                                                                                        tGCACATTTCTCGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTACGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa                          <  2:952533/143‑1 (MQ=255)

                                                                                                              ttACCAGTACCGAAGAATATCCTTGTATCCAACTGCTTATCTAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACTCTTCTTAAGCGAACTGACCGCCTCTGAAGGTTTTGAACGTTACCTCGGCGCAAAATTCCCt                    <  2:2466/143‑1 (MQ=255)

                                                                                                                          aagaaAAACTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGCAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCCCTGAAGGTCCTGAACGTTACCTCGGCGTAAAGTTCCCTGGCGCACAACGc        >  2:908477/1‑143 (MQ=255)

                                                                                                                            gaaACACTCTGTATCCCACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATCGCGAAGCGAAAAAACGCTTCTTAATCGAACTGACCGCCGCTGAAGGGCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt      <  1:235863/143‑1 (MQ=255)

                                                                                                                                aaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGGCGCAAAACGCTTCTCg  >  1:495/1‑143 (MQ=255)

CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG  >  NC_000913/759119‑759389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A