Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F87 I0 R1
|
87 |
48.8 |
1893308 |
89.3% |
1690724 |
142.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
100% |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 98.6%
| 105.4
/ ‑6.6
| 39 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (18/19); minor base C (2/0); total (20/19) |
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759120‑759387
|
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATCACCAGCACCGAACAAAAACCCCGGATCCAACa > 2:434856/1‑143 (MQ=255)
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGACGAGAAACCCTGGATccacca > 2:255544/1‑143 (MQ=255)
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACTCCGGAACCAACa > 2:405926/1‑143 (MQ=255)
ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGa < 1:601547/143‑1 (MQ=255)
ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGACTGGTGCCGAATATATGCACAATACAAGCACCGAAAAACAACTCTGGATCAAACAGcatcacgc > 2:552201/1‑136 (MQ=255)
gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg > 1:631628/1‑143 (MQ=255)
gTTCATTCGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg < 2:125808/143‑1 (MQ=255)
ttctgctGCCAGCGGCAAATAAACCACGCACCTGGGCGAGCTGCGGGACGCCCTCAAGCAAACCTACTGCGGCCCGATGAGTGCCGAGTATATGCACATTACATGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 2:647946/137‑1 (MQ=255)
ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAgg > 1:662571/1‑142 (MQ=255)
aTTTGCCAGCGGCAAAGAAGCCAGGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg < 2:398839/143‑1 (MQ=255)
tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg > 1:243113/1‑143 (MQ=255)
tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg < 1:295919/143‑1 (MQ=255)
cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACCGCGTATCGAGTCTGGTcgcg > 2:731055/1‑143 (MQ=255)
gAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGAATCCAACAGCGTATCGAGTCTGGTCGCGCGCCTTTCaa > 1:637280/1‑143 (MQ=255)
aaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCACGCAAACCTGCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATa < 1:529281/143‑1 (MQ=255)
aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:134456/143‑1 (MQ=255)
aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:906029/143‑1 (MQ=255)
gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc > 2:294336/1‑117 (MQ=255)
gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 1:294336/117‑1 (MQ=255)
ggCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACACTACCAGCACCGAAGCAACACTCTGGCTCCAACACCGCCCCGAGTCTCGCCGCGCCACCTTCAATAGCGACGAGaaaaa > 2:218673/1‑143 (MQ=255)
gAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACg < 1:183932/143‑1 (MQ=255)
aGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACACTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc < 1:158733/143‑1 (MQ=255)
aGCCGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGc < 1:239124/143‑1 (MQ=255)
gctgcgGGAAGCCCGCAAGCAAGCCTACGGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCAAACAGCGTATCGAGTCTGGTCGCGCGACTGTCAATAGCGAAGAGAAAAAACGct < 2:815028/143‑1 (MQ=255)
ctgGAAGCCCCAGAGCCAAGCTCCTGCGGCCAGTGGGGTGCCGGGTATATGCACAGTACCAGTACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt < 1:391899/143‑1 (MQ=255)
gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGAACATTACCAGCCCAGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGAGCGACTTTGCAGAGCGAAGAGAAAAAACGCTTCTTAAg > 2:930245/1‑143 (MQ=255)
aaccAAACCTACTGCGCCAGCATTGTTGCCGCGCTGGTCCGCATGCCCAGCACCGAAGAAAAACTCTGGATCTAACAGCGTATCTAGTCTGTTCGCGCGACTTCCAATACCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:914618/140‑1 (MQ=255)
cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTg > 1:781299/1‑143 (MQ=255)
tgcggCGGGCCGGTTGCGGCCGGGGATATGCACATTACCAGCAGCGAAGAAAGGCTCTGGATCCGACAGGGTAGCGAGGCTGGTCGCGCGACGTGCAATGGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGa < 2:306632/139‑1 (MQ=255)
ccgCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:712765/141‑1 (MQ=255)
cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGGGTATCGACTCTGGCCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGATGaaagac > 2:236222/1‑139 (MQ=255)
cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGGCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGCAGGTCTTg > 1:353947/1‑143 (MQ=255)
tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGCAGGTCTTGAACGTTAcc > 1:145842/1‑143 (MQ=255)
aGTATGTGCACATAACCAGCAGCAAAGAAAAGCTCTGGATCCAAGAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 2:513090/143‑1 (MQ=255)
acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGCCTTTCAAGAGCGAAGAGAAAAGACGCATCGTAGCCGAACTGACCGCCGCTGAAGGTCTTGACCGTTACCTCGGCCCaagcttc > 1:215334/1‑138 (MQ=255)
cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa > 1:679755/1‑143 (MQ=255)
gaagaaACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGCTACCTCGGCGCAAAATTCCCTGGCGCAAAACg < 1:221745/143‑1 (MQ=255)
gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCACAACg > 1:594982/1‑143 (MQ=255)
aaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCATGACAGATAACCCTGCGCACAACTCCCTGGCACAAAACGCAtct > 2:661596/1‑143 (MQ=255)
|
TTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759120‑759387
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
