Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F51 I1 R1
|
25 |
46.9 |
1811498 |
90.3% |
1635782 |
141.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 93.5%
| 117.4
/ NA
| 46 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base T (24/19); total (25/21) |
| Fisher's exact test for biased strand distribution p-value = 5.85e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388
|
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 2:529008/143‑1 (MQ=255)
caagagaCCTTCAACGTCGGTGCATCTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATAGGGGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTg < 2:724675/140‑1 (MQ=255)
gagaCCTTCACCGTCGGTGCATTGTCCAGCGGCAAAGAAACCATGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAt < 2:565742/143‑1 (MQ=255)
aggccTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATc < 1:173583/140‑1 (MQ=255)
tCGGTTCATTGGCCGGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 1:506418/143‑1 (MQ=255)
ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 1:255641/143‑1 (MQ=255)
tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg > 1:875323/1‑143 (MQ=255)
cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg > 1:597930/1‑143 (MQ=255)
aGCGGGAAAGAAACCCGGAATCGCGCTGAGCTGCTGGAAGCCCTCAAGCAATCCTACTGCGGCCCGATTGGTGCCGATTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGAGTCTGGTcgcgc < 2:302391/143‑1 (MQ=255)
cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa > 1:428874/1‑143 (MQ=255)
gCAAAGAGACCATGGAATTTTTCAAGCTGCTGGAAGCCCTCAAGCCAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACt < 1:846946/143‑1 (MQ=255)
gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGGTCCAACAGCGTATCGAGTCCGGTCGCGCGACt > 1:460463/1‑143 (MQ=255)
cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCCCAAGCAAACCTACTGCGGCGCGGGTGGTGCCGAGAATATGCACATTGCGAGCACCGAAGAAAAACGCTGGATCCCACAGCGCACCGAGTCTGGGCCCGCGACta > 2:191741/1‑142 (MQ=255)
aacgAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt < 1:50481/140‑1 (MQ=255)
gAAACCATGAAACCCGGCGAGCCGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCaa < 1:65110/143‑1 (MQ=255)
aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAt > 1:254197/1‑143 (MQ=255)
cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTCACAATAGCg > 2:220166/1‑143 (MQ=255)
aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGAACCGAAGAAAAACTCTGGATcc < 1:87696/98‑1 (MQ=255)
aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGAACCGAAGAAAAACTCTGGATcc > 2:87696/1‑98 (MQ=255)
tCGGCGAGCTGCTGGAAGCCCTCAAGCAAAACTACTGGGGAACGATTGTTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCATGGTCGCCCGACTTGCAATAACGAAGAGaaa > 2:644529/1‑143 (MQ=255)
gCGAGCGGCTGGACGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCATCACCGACGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa < 1:705990/143‑1 (MQ=255)
ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAAGCGCTTCTTaa > 1:829613/1‑143 (MQ=255)
gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGGGCGGCTCTAAATATCGTAGAGAAAAAACGCTTCTTAAg > 1:374669/1‑143 (MQ=255)
aGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg > 1:303552/1‑143 (MQ=255)
aGCCCGCGAGCCAACCTTCTGTGGCCCGTTTGGTGCCGACGATACGCACATTGCCAGCACCGAACAACAACTCTGGATCCTACCGCGTATCTAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg < 2:643458/143‑1 (MQ=255)
cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCCCATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGCAGCGGAAAAACGCTTCTTACGCGCACt > 1:439381/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTCTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 2:864443/1‑143 (MQ=255)
tgaaaaCTACTCCAGCGGGATTGCCGCCGACGATAGTCACATAACCAGAAACAAAAAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccg < 2:657608/141‑1 (MQ=255)
aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGAACATTACCCGCACCGACTAAAAACTCTGGATCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgccg > 1:322803/1‑143 (MQ=255)
aCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCGGCACCGAGGAAAAACTCTGGATACAACAGCGTATCGACTCTGGTCGCGCGGCTTTCAATAGAGACTAGAACAAACGCTTCTGAAGACAACTGgccgccacagac > 2:64404/1‑137 (MQ=255)
aCTGCGGCCCGATTGGTGCCGAGTATACGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa < 1:724895/143‑1 (MQ=255)
ggTGCCGAGTATACCCACTTCACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 2:428493/143‑1 (MQ=255)
ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTc > 1:877927/1‑143 (MQ=255)
ttcacaCTACCTGCACCGAAGTAGACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa < 2:567150/141‑1 (MQ=255)
cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAACACGCTTCTTACGCGAACTGACCGCCGCTGAAGGTCTTGCACGTTACCCCCGCGCaacgca > 2:40548/1‑139 (MQ=255)
agtACCTGCAGCGAAGAAAGTCGCTGGATCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCCATCGCGATAATAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc < 2:271168/141‑1 (MQ=255)
aCCAGCACCGAAGAAAAGCTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAGTCCCTgg < 2:260632/143‑1 (MQ=255)
ccAGCCCCGGACAAAAGCGCTGGATCCAACTGCGTATCGAGTGTTTTCGCGCGACTATCACCAGACATGAGAAAACCCGCTTCTTACGCGAACCGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 2:677471/143‑1 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACg > 1:76707/1‑117 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGgcg > 1:815366/1‑143 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACg < 2:76707/117‑1 (MQ=255)
aggacggaagaaAGACTCTGGATCCAGCAGCGTGTCGAGTTGGGTCGCGGGACTTGCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGGAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc < 2:194955/137‑1 (MQ=255)
gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAACGCCCTGGCGCCCAACg > 1:216845/1‑143 (MQ=255)
aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGGGGGTGTTTCCATAGCGAAGAGCAAAAACGCTTCTTAAGCGGACTGACCGCAGCTGCAGGTCTTGCACGTTCCCTCGGCTCCAAATCCCCTGGAGCACAAccc > 2:494639/1‑141 (MQ=255)
gaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt > 1:621757/1‑143 (MQ=255)
aaaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGACTTGAACGGTACCTCGGCGCAAAAGTCCCGCGCGCAAACAGCTtc > 2:652700/1‑143 (MQ=255)
aaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTCCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTAACCTCGGCGCAAAATTCCCTTGCGCAAAACGCTtctc > 2:313396/1‑143 (MQ=255)
|
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
