Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F20 I1 R1	20	59.7	2309458	89.5%	2066964	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,250	G→T	R182L (CGC→CTC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,250	0	G	T	97.7%	114.0 / NA	44	R182L (CGC→CTC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (22/20); minor base C (1/0); total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT  >  NC_000913/759113‑759385

ggAGACCTTGAACGTCGGTTCAGTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa                                                                                                                                    <  1:872386/143‑1 (MQ=255)

            cGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTa                                                                                                                        <  1:540843/143‑1 (MQ=255)

              tCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCTTCAAGCATACCTACTGCGGCCCGATTGGTGACGAGTATATGAACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc                                                                                                                      <  1:192992/143‑1 (MQ=255)

                 gatCATTTGCCAGTGGCAAAGAAACCATGAAACTAGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAACTGCACAATACCAGCACCGAAGACAAACTCCGGAGCCAACAGCGCAACGAg                                                                                                                   >  2:26801/3‑143 (MQ=255)

                 gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGTTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg                                                                                                                   >  1:796649/1‑143 (MQ=255)

                  ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCAAAGAAAAACCCTGGAACCAACAGCCTACCGAgg                                                                                                                  >  2:1026707/1‑142 (MQ=255)

                     cgttGGCAGCGGCAAAGAAACCGTGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg                                                                                                               <  1:362974/141‑1 (MQ=255)

                              cGGCAAAGAAACCATGACACGCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa                                                                                                      <  1:167920/143‑1 (MQ=255)

                                    aGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCCGGGCGCGCGACTTTCa                                                                                                >  1:401371/1‑143 (MQ=255)

                                           aTGATAGTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGa                                                                                         <  1:197926/143‑1 (MQ=255)

                                              aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagc                                                                                      >  1:795819/1‑142 (MQ=255)

                                               aaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagag                                                                                     >  1:999381/1‑143 (MQ=255)

                                                 cTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGGACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACGTGCACTAGCGAAGAGaa                                                                                   >  1:602410/1‑143 (MQ=255)

                                                     gCGAGCTGCTGGAAGCCCTCAAGCAAACCTGCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa                                                                               <  1:1122263/143‑1 (MQ=255)

                                                            gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttct                                                                        >  1:35483/1‑143 (MQ=255)

                                                                gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg                                                                    <  1:1152037/143‑1 (MQ=255)

                                                                    cccTCAAGCAAACCTACGGCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa                                                                <  1:504210/143‑1 (MQ=255)

                                                                    cccTCAAGCAAACCTACCGCCGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGACTCTGGACGCGCGACTTTCTATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa                                                                <  2:600883/143‑1 (MQ=255)

                                                                    cccTCAAGCAAACCGACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa                                                                <  2:1025253/143‑1 (MQ=255)

                                                                      cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt                                                              >  1:806888/1‑143 (MQ=255)

                                                                              aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAGTGTGGATGCAACAGCGAAGCGAGGGGGGGCGCGCGACGTTGAATAGCGGAACGAAAAAAAGCGGCGTGAGCGGACTGGgcgccg                                                      >  1:456178/1‑143 (MQ=255)

                                                                                    cTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                <  2:796649/143‑1 (MQ=255)

                                                                                         gCCGGATTGGTGCCGAGTATATGGACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt                                           <  1:861798/143‑1 (MQ=255)

                                                                                          cccGGTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                          <  2:748343/143‑1 (MQ=255)

                                                                                          cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTCCCATAGCGCAGCGGCAAACCGCTTCTTCAGCGAACTCAACGCTGCTGAAgataata                                          >  1:870163/1‑137 (MQ=255)

                                                                                            cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGCTTCTCAAGCGAACTGACCGCCGCGGCAggccctgcc                                        >  1:603325/1‑136 (MQ=255)

                                                                                             gcttGGTGCTGGGTGTATGCACATTACCAGCGCCGAAGAACAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAAc                                       <  2:1028262/141‑1 (MQ=255)

                                                                                               ttGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGACGAGAAAAAACGCTTCTTAAGCGAACTGACCGCGGCTGAAGGGCTGGAAcct                                     >  2:113107/1‑141 (MQ=255)

                                                                                               ttGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGt                                     <  2:311438/143‑1 (MQ=255)

                                                                                               ttGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATACAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTACCGAAGAGACAAAACGCTTCTTAAGCGAACTGCCCGCCCCTGCAGGACTTGAACGt                                     >  2:14722/1‑143 (MQ=255)

                                                                                                  gTGCCGAGTATATGCACATTACCAGCAACGAAGGAAAAAACACGAACCAACAGCATATCGAGTAAGGTCGATCAGCTGACAATAGCGAAGACAAAAAACGCCACTTAAGCGAACTGACCGCCCCCGAAGGCCTTGAAAGGTAc                                  >  2:1004186/1‑143 (MQ=255)

                                                                                                    gCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAACGTTACCt                                >  2:467003/1‑143 (MQ=255)

                                                                                                     cgtaGTATATGCACACTACGACGACTGCACAAAAACTCTGGATCGAACAGGGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTc                               <  2:780983/140‑1 (MQ=255)

                                                                                                     ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGACGCGCGACTTTCAATAGCGAAGAGAAAGAACGCGGCTTAAGCGAACTGACCGGCGCTGAAGGCAGTGAACGCTagcca                               >  2:1029195/1‑139 (MQ=255)

                                                                                                              atcacaTTGACAGGACCGAAGAAGAACTCTTGATTCAAAAGAGTATCGAGTCTGGTCGCTCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGGTGAAGGTTTTGAACGTTACCTCGGCGCaaaa                      <  2:1038517/141‑1 (MQ=255)

                                                                                                               gCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGACAAAACGCTTCTTAGGCGACCTGACCGCCGCTGAAGTGATTGAACGTTACCTGGCCGCaacgt                     >  2:1104424/1‑140 (MQ=255)

                                                                                                                cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACGTTCAATCGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAtt                    >  1:373831/1‑143 (MQ=255)

                                                                                                                 acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGGAGGTCTTGAACGGTCCCTCGgcgctcacttc                   >  1:169383/1‑136 (MQ=255)

                                                                                                                   aTTACGAGCGCCGAAGAAAAACTCCGGAACCAACAGGGTATCGAGGCTGGGCGCGCGACTGTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTccc                 <  1:19612/143‑1 (MQ=255)

                                                                                                                    ttACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGACCGTTACCTCGGCGCAAAATTCACt                >  2:799296/1‑143 (MQ=255)

                                                                                                                        cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGACTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGATACCTCGGCGCAAAATTCCCTGgcg            >  2:886386/1‑143 (MQ=255)

                                                                                                                         aGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc           >  2:123736/1‑143 (MQ=255)

                                                                                                                          ggaCCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCTCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCa          <  1:972185/141‑1 (MQ=255)

                                                                                                                                  gaaAAACTCTGGATCCAACAGCGTATCGAGTCAGGTCGCGCGACTTTCAATCGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt  >  2:602202/1‑143 (MQ=255)

GGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT  >  NC_000913/759113‑759385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A