Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F91 I0 R1
|
49 |
33.8 |
1296440 |
89.8% |
1164203 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
100% |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 100.0%
| 71.7
/ ‑6.7
| 28 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (13/13); total (14/14) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCG > NC_000913/759119‑759375
|
cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGGTTGGTGCCGAGGATATGCACATTACCAGCACGGCAGAAAAACCCTGGATcccag > 2:586970/1‑140 (MQ=255)
tCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 2:604490/143‑1 (MQ=255)
tgcttggCCAGCAGCAAAGAAGCCAGGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATCTGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 2:416220/137‑1 (MQ=255)
tCATTTGCCAGCGTCAAACAATCCATGAACCTCTGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCTGCCCTATTGGTGCCTAGTATATGCACATTACCTGCACCGAAGAAATTCTCTGGATCCAACAGCGTATCGAGTc < 1:352670/143‑1 (MQ=255)
cAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTagcg > 1:279523/1‑143 (MQ=255)
ggCAAAGAAACCGTGAAACTCGGCGAGTTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCTGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 2:415752/143‑1 (MQ=255)
ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 2:547331/143‑1 (MQ=255)
cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTCCCGAGTATATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCACCCGCGTATCGAGTCTGGTCGCGCGACtt > 1:255327/1‑143 (MQ=255)
gcatccATGCTACTCGGCGAGCTGCTGGAAGCCCTCCAGCACACCTACTCCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCaa < 1:225273/139‑1 (MQ=255)
aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAt > 1:108455/1‑143 (MQ=255)
aCCATGAAAGTCGGGGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg < 1:85741/143‑1 (MQ=255)
tgcaaCTCGGCGAGCTGGTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATGACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa < 1:630785/140‑1 (MQ=255)
ccGGCGAGCTGCTAGACCCCCTCATGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa < 2:190082/142‑1 (MQ=255)
ggCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGCGaaaaa > 1:400877/1‑143 (MQ=255)
ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTAGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa > 1:54575/1‑143 (MQ=255)
ggAAGCCCGCACGCAAACCTACTGCGGCCCGAGTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACTCTGGATCGAACAGAGTATCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 2:149269/143‑1 (MQ=255)
aaGCCCTCAAGCAAACCTACTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc < 1:586111/143‑1 (MQ=255)
aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAAAGCGAAGAGAAAACACGCAACTTAAACCAACTGAccgccg > 2:590522/1‑143 (MQ=255)
aaCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCGGGCCACGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgccg > 1:471183/1‑143 (MQ=255)
gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAACAGCAAACACGCTTCCTAAGCCAACTGACCGCCGCTGAAGGt > 2:277291/1‑143 (MQ=255)
tgCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGACCCAACAGCGTAACGAGACTGGTCGCGCGACTTTCAATAGCGAAGAGATAAAACGCTTCGTAAGCGAACTGACCGCCGCTGAAggccc > 2:617200/2‑140 (MQ=255)
cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAGAACGCTTCTTGAGCGAGCTGACCGCCGCTGAAGGTCTTGaa > 1:229906/1‑143 (MQ=255)
gATGGGTGCCGATTATATGCACATTGCCGGCACCGAGGAAACACTCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGGGAAGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTGTTGAAc < 2:263484/143‑1 (MQ=255)
tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc > 1:401704/1‑143 (MQ=255)
tatGCGCATTACCACCACCGAAGAAAAACGCTGGTGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaa < 2:526264/143‑1 (MQ=255)
cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAtt > 1:463632/1‑143 (MQ=255)
ccAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 2:274127/143‑1 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcc > 2:26865/1‑142 (MQ=255)
|
CTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCG > NC_000913/759119‑759375
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
