Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F25 I1 R1
|
24 |
37.7 |
1533230 |
90.5% |
1387573 |
141.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 95.7%
| 59.9
/ NA
| 24 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (13/9); total (14/10) |
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC > NC_000913/759110‑759386
|
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 1:722962/143‑1 (MQ=255)
aaCGTCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCg < 2:1460/143‑1 (MQ=255)
tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc < 1:357569/143‑1 (MQ=255)
ccATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACATACTGCGGCCCGACTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGCTCCAACAGCGTCTCGAGCCGGCTCGCCCCACTATCaccagc > 2:604350/1‑138 (MQ=255)
aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAgaga > 1:563415/1‑143 (MQ=255)
ctgctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt > 1:704608/1‑143 (MQ=255)
tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGGGGCTGGTCGCGCGACTTGCAATAGCGAAGAGAGAAAACGCGGCTTa > 1:196605/1‑143 (MQ=255)
tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTa < 1:53561/143‑1 (MQ=255)
cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt > 1:35079/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAACCAACTg > 2:142104/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGATGAGAAAAAACGCTCCTGAAGCGAACTg > 2:586824/1‑143 (MQ=255)
aaCCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccgccg < 2:554438/143‑1 (MQ=255)
atggCGGCCAGCTTGGTGCCGGGGATATGCGCATTACCAGCCCGGAAGAAAATGTCTGGATCCAACAGCGTATCGATTCTGTTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAATCGAACTGACCGCCGCGGaa < 2:518712/140‑1 (MQ=255)
gCCAGATTGGGCCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 1:472668/143‑1 (MQ=255)
tGCCGAGTATATGCACATTACCAGCACCGAAGACAGGAACTAAAATCATCGGCGGATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc > 1:146021/1‑143 (MQ=255)
aGTATATGCACATGACCAGCACCGAAGCAAAACTCTGGCTCCAACAGCGTATCGAGTCTGGTCGCGCGCCTTTCCATAGCGAAGAGCGAATACGCGTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGCACGTTACCTCGgc > 2:628404/1‑143 (MQ=255)
gatGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaa < 2:563415/142‑1 (MQ=255)
gCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc > 1:60332/1‑46 (MQ=255)
gCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 2:60332/46‑1 (MQ=255)
cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc > 2:11776/1‑40 (MQ=255)
cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 1:11776/40‑1 (MQ=255)
acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc > 1:709219/1‑143 (MQ=255)
ccttcccAGCACCGAAGAAGAACTCTGGATCCCACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGCCCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 1:403701/138‑1 (MQ=255)
aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAGAATTCCCTGGCGCAAAACGc > 1:569424/1‑143 (MQ=255)
aaaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtc > 1:453185/1‑143 (MQ=255)
|
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTC > NC_000913/759110‑759386
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
