Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F87 I1 R1 26 57.4 2238216 89.7% 2007679 140.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 759,250 G→T R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,2500GT92.3% 135.8 / ‑5.5 53R182L (CGC→CTC) sucAsubunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (26/22);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT  >  NC_000913/759110‑759385
                                                                                                                                            |                                                                                                                                       
ccAGGAGACCTTCACCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                       <  2:1015867/143‑1 (MQ=255)
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                       <  2:784970/143‑1 (MQ=255)
     agaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACGCTGGATc                                                                                                                                  >  1:903448/1‑143 (MQ=255)
     agaCCTTCAACGTCGGTTCAGTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATc                                                                                                                                  <  1:727085/143‑1 (MQ=255)
      gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATcc                                                                                                                                 <  1:333688/143‑1 (MQ=255)
            cAACGTCGGTTCATTTGCCAGCGGCGAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAGACGCTGGCTCCGACAGc                                                                                                                           >  1:1035313/1‑143 (MQ=255)
              aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATCAGTGCCGAGTATATGGACCTTACCAGCACCGACGAAAACCCCTGGATACGACAACgc                                                                                                                         >  1:677399/1‑142 (MQ=255)
                 tCGGTTCATGTGCCCGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc                                                                                                                      <  1:946019/143‑1 (MQ=255)
                    gTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGTGCCGAGTATATGCACATTACCAGCGCCGAAGCAAAACGCTGGATCCAACAGCGCACCGAg                                                                                                                   >  2:647980/1‑143 (MQ=255)
                     ttCATATGCCAGCCGCAGAGAAACCATGAAACTCCGCGACGTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt                                                                                                                  <  2:521836/143‑1 (MQ=255)
                       cATTTGCCAGCCGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCt                                                                                                                <  1:132240/143‑1 (MQ=255)
                         tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTgg                                                                                                              >  2:749302/1‑143 (MQ=255)
                         gtacccAGCGGCAAAGACACCATGAACCTCGGCGAGCGGCGGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTgg                                                                                                              <  1:648402/139‑1 (MQ=255)
                           tGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATACAAAAGCGTATCGAGTGTGGTc                                                                                                            >  2:208257/1‑143 (MQ=255)
                             ccAGCGGCAAAGAACACATGGAAGTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgc                                                                                                          <  2:829944/143‑1 (MQ=255)
                             ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACTCTGGATCCAACAGCGTATCAAGTCTGGTagc                                                                                                          >  1:443082/1‑143 (MQ=255)
                                  ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc                                                                                                     <  1:612861/143‑1 (MQ=255)
                                   gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACt                                                                                                    >  1:607325/1‑143 (MQ=255)
                                    cgaagaaaccaagaaaccCGGCGAGGTGCTGGAAGCCCGCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtt                                                                                                   <  1:298557/141‑1 (MQ=255)
                                    cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGGTCGCGCGACtt                                                                                                   >  1:841682/1‑143 (MQ=255)
                                     aaaGAAACTATGAAGCTCGGCGGGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt                                                                                                  <  2:419606/143‑1 (MQ=255)
                                     aaaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTATAGATACATCAGCGTATCGAGTCTGGTCGCGCGACttt                                                                                                  >  2:229340/1‑143 (MQ=255)
                                      aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAGATGCACATTACCAGCACCGCAGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc                                                                                                 >  1:996253/1‑143 (MQ=255)
                                           aCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAg                                                                                            <  1:966799/143‑1 (MQ=255)
                                               tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCTGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa                                                                                        >  1:116299/1‑143 (MQ=255)
                                                gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTGTGGATCCAGCAGCGTATCGAGTCTGGTCGCGCGACTTTCAATCGCGagg                                                                                       >  1:421255/1‑141 (MQ=255)
                                                 aaaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAaga                                                                                      <  1:889064/143‑1 (MQ=255)
                                                   actgcgCGAGCCGCTGCAAGCCCTCAACCAAACCTCCTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCACCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAgaga                                                                                    <  2:1017347/138‑1 (MQ=255)
                                                   aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACACTACCAGCACCGAAGAAAAACTCTGGCTCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagagc                                                                                    >  2:39311/1‑142 (MQ=255)
                                                        tctaGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTGCCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa                                                                               <  1:983009/140‑1 (MQ=255)
                                                                ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt                                                                       <  1:1086524/143‑1 (MQ=255)
                                                                ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAAACTCTGGGTCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGGAACAACGcttctt                                                                       >  1:899570/1‑143 (MQ=255)
                                                                 tgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGcttcttc                                                                      >  1:1025364/1‑142 (MQ=255)
                                                                   gAAGCCCTCACGCAAACCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg                                                                    <  1:1012014/143‑1 (MQ=255)
                                                                       cccTCAAGCACACCGACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa                                                                <  2:1013694/143‑1 (MQ=255)
                                                                         cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt                                                              >  1:107905/1‑143 (MQ=255)
                                                                          tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTAACAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTGCTTAAGCGAACTg                                                             >  1:1075962/1‑143 (MQ=255)
                                                                               cAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAGCAGCGTATCGAGTCTGGTCGCGCGACTGTCAATAGCGAAGAGAAAAAACGCTTATTGAGCGACATGAcaac                                                        >  1:482040/1‑140 (MQ=255)
                                                                                    cTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTg                                                   >  2:1116074/1‑143 (MQ=255)
                                                                                       cTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCAGCGATGGGAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                <  2:1025364/143‑1 (MQ=255)
                                                                                         gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGTAGTGAAAAAACGCTTCTGAAGGGAACTGACCGCCGCTCAAGGt                                              >  1:243169/1‑143 (MQ=255)
                                                                                                     gTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAc                                  >  1:805231/1‑143 (MQ=255)
                                                                                                           agcatacGCACATCACACGCACCGAACAAAAACTCTGGATCCAACCGCGTATCTAGTCTGGTCGCGCGACCTTCAATAGCGAAGAGAAAAACCGCCTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc                            <  1:882472/140‑1 (MQ=255)
                                                                                                                      aTTACCAGCACCGAAGAAAAACTCTAGATCCAACAGCGTATCGAGTCTGGTAGCGCGACTATCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTAAAGGTCTTGAACGTTACCTCGGCGAaaaaacacc                 >  2:47956/1‑138 (MQ=255)
                                                                                                                         gccAGCACCGAAGAAACGCTCTGGATCCAACAGCGTGTCGAGTCTGGTCTCGCGACTTTCAATAGCGAAGAGAAAAACCGCTTCTTAAGCGAACTGACCGCCGCTCAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTgg              <  2:873040/142‑1 (MQ=255)
                                                                                                                           cAGCACCGAAGAAAAACTCTGGATCTAACAGCGTATCGAGGCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGCCAGTTACCTCGGCGCCGAATTCCCTGgcg            >  2:802510/1‑143 (MQ=255)
                                                                                                                           cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGGAGAGACAAAACTCATCTTACAAGGCAGGACCTCCGCGGAAGATCTTGACCGTTACATCGGCGCAAAATTCCCTGgcg            >  2:1086077/1‑143 (MQ=255)
                                                                                                                            accACCGAAGAAAAACGCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgc           <  2:1070519/141‑1 (MQ=255)
                                                                                                                              cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCGCGGCGCACAACTCCCTGGCGCac         >  2:754279/1‑142 (MQ=255)
                                                                                                                              cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcgcgc         >  1:69822/1‑141 (MQ=255)
                                                                                                                                ccGAGGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAACCGCTGCTTAAGCGAACTGACCGCCGCTGACGGTCATGAACGCCACCTCGGCGCAAAATTCCCTGGCGCaaaa       <  2:677399/143‑1 (MQ=255)
                                                                                                                                 cGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAc      >  1:13754/1‑143 (MQ=255)
                                                                                                                                   aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGc    >  2:387533/1‑143 (MQ=255)
                                                                                                                                   aagaaAAACTCCGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGc    <  1:647980/143‑1 (MQ=255)
                                                                                                                                     gaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt  >  1:596367/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                       
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTT  >  NC_000913/759110‑759385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A