Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F61 I1 R1	22	65.1	2541758	91.4%	2323166	141.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,250	G→T	R182L (CGC→CTC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,250	0	G	T	98.0%	138.3 / NA	50	R182L (CGC→CTC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (27/21); total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG  >  NC_000913/759110‑759389

ccagcggaCTTTCTACGTCGTTTCACTGGGTGGCGGCAAAGAAACCATGAATCTCGGCGAGCTGCTGGAAGCCCGCAAGCAATCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACActct                                                                                                                                             <  2:357758/137‑1 (MQ=255)

ccAGGAGCCCTTCGACGTCGGTTCAGTTGCCAGCGGCAAAGAAACCATGAATCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct                                                                                                                                             <  2:1055592/143‑1 (MQ=255)

 cgacagcccTTCGACTTCGTTTCATTGTCCATCGGCAAAGAAACCATGACACTCGGCGAGCTCCTGGAAGCCATCAATCAAACCTACCGCTGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAATACTCTg                                                                                                                                            <  2:173072/136‑1 (MQ=255)

   ggggaCCTTCAACGTCTGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGa                                                                                                                                          <  1:933721/140‑1 (MQ=255)

          ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa                                                                                                                                   >  1:245007/1‑143 (MQ=255)

              aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGATCTGCTGGGAGCCCTCAAGCAAACATGCTGGGGCCCGATTGGGGCCGAGTATAGGAGCATTAGCAGCACCGGAGAAAATCTCTGGATCCTGCAGCga                                                                                                                               >  2:6590/1‑142 (MQ=255)

                      tCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTc                                                                                                                       <  1:9814/143‑1 (MQ=255)

                               aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑Tc‑gcgc                                                                                                            <  1:17692/143‑1 (MQ=255)

                               aGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAACCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGACGCAAAACTCTGGATCCAACAGCGTCCCGCCTCTAG‑Tc‑gcgc                                                                                                            >  2:232010/1‑143 (MQ=255)

                                 cGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGa                                                                                                          <  1:1026363/143‑1 (MQ=255)

                                  ggCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTGAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGAc                                                                                                         <  2:1000091/143‑1 (MQ=255)

                                      aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTCGATCCAACAGCGTCTCGAGTCTGG‑TC‑GCGCGCCttac                                                                                                     >  2:861392/1‑141 (MQ=255)

                                        gAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAACCTCTGGAACCAACAGCGTCTCGAGCCTGG‑TC‑GCGCGACTCTCaa                                                                                                   >  2:484633/1‑143 (MQ=255)

                                            ccATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGc                                                                                               <  1:625319/143‑1 (MQ=255)

                                                     tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGG‑AC‑GCGCGACTTGCAATAGCGAAGAGaaa                                                                                      >  2:1161968/1‑143 (MQ=255)

                                                        gctaGCTGCTGCAAGCCGTCAAGCAACGCTCCTGCGGCCGGATGGGTGCCGAATATAGGCACATTACGCGCACCGACGAAAAACTCTGAATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGaaaaaa                                                                                   <  2:1185083/140‑1 (MQ=255)

                                                         cgggctgctGGAAGCCCCCGCGCCAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAAc                                                                                  <  2:822802/140‑1 (MQ=255)

                                                             ctgctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGctt                                                                              <  1:196508/143‑1 (MQ=255)

                                                                ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt                                                                           >  1:664118/1‑143 (MQ=255)

                                                                cgggAAGCACTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt                                                                           <  1:575066/141‑1 (MQ=255)

                                                                  ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa                                                                         <  1:1032202/143‑1 (MQ=255)

                                                                   gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCCCCTTACTAGCGCCGAAGCCACACGCCGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTGCAATAGCGAAGAGACAAAACGCTTCGTAAg                                                                        >  2:1235252/1‑143 (MQ=255)

                                                                   gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAGACTCTGGATCCAACAGCGTATCGAGTCTGG‑TG‑GCGCGACTCTCAATAGCGAAGAGAAAAAACGCTTCGTAAg                                                                        >  1:224174/1‑143 (MQ=255)

                                                                        ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGCGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TA‑GCGCGACTTTCAATAGAGACGAGAAAAAACGCTTCTGAAGCAAAc                                                                   >  2:823274/1‑143 (MQ=255)

                                                                        ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAAc                                                                   >  1:907221/1‑143 (MQ=255)

                                                                         cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt                                                                  >  1:29386/1‑143 (MQ=255)

                                                                              gCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTGAAGCGAACTGAccg                                                             >  2:660208/1‑143 (MQ=255)

                                                                                       cTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                    <  1:563443/143‑1 (MQ=255)

                                                                                        tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGCGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAgg                                                   >  2:186915/1‑143 (MQ=255)

                                                                                         gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTACTTACGCGAACTGACAGCCGCTGAAGGt                                                  >  2:536836/1‑143 (MQ=255)

                                                                                          cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑GC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc                                                 >  1:129424/1‑143 (MQ=255)

                                                                                              cctaTTTGTGCCGAGTCTATGCACGGTACCAGCACCGAAGAAAAACTCTGGATCTAACAGCGTATCGAGTCTGG‑CC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGTCCGCCGCTGAAGGTCTTGa                                             <  2:62461/140‑1 (MQ=255)

                                                                                               cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TA‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTCCTTAAGCGAACTGACCGCCGCTGAAGGTCTTgca                                            >  2:1135573/1‑141 (MQ=255)

                                                                                                 aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGATGACGGTCATGAACg                                          >  1:463641/1‑143 (MQ=255)

                                                                                                      tGCCGAGTATATGCACATTACCAGCATCGAAGAAATTCTTTTGATCTAACATTGTATCGAGTCTGG‑TC‑GCGCGAGTTTCAAGAGCGAAGGGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc                                     <  1:781892/143‑1 (MQ=255)

                                                                                                      tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑GC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGCACTGACCGCCCCCGCAGGTCTTGCACGTTAcc                                     >  2:462645/1‑143 (MQ=255)

                                                                                                         ggggTATAGGCAGATTACGAGCACCGAAGAAAAGGTGTGGAGCCAACAGGGTGTAGAGTCGTG‑TC‑GCGCGACGTTAAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGACGCTGAAGGTCTTGAACGTTACCTCg                                  <  2:300630/140‑1 (MQ=255)

                                                                                                         cGAGTCTATCCACCTTACCTCCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATCGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAATGTCTTGAACGTTACCTCg                                  <  2:1244986/143‑1 (MQ=255)

                                                                                                          gAGTATATGCACATTACCAGCACCGAAGAAAAACTAAGGATCCAACAGCGTACCAAGTCTGG‑TC‑GAACGACGAGCAATAGCGTAGAGGCAAAACGCTACTACAGCGAAGTGACCACGGCTGGAGGTCTTAAACGTTATCTCgg                                 >  2:1028374/1‑143 (MQ=255)

                                                                                                           aGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGCCTTTCAATAGCGAAGAGAAAAAACGCTTCTTCAGCGAACTGACCGCCCCTGAAGGTCTTGCACGTTCACTCGgc                                >  2:737686/1‑143 (MQ=255)

                                                                                                           aGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc                                <  1:1245275/143‑1 (MQ=255)

                                                                                                             catatGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGAACAGAGCGAC‑‑CGGAGAGGGGAGAGAGCAAACCCCTCTTAACCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgcgc                              <  1:294770/142‑1 (MQ=255)

                                                                                                                  gCACATTACCAGCACCGAAGAAAAACCATGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAACGATACCTCGGAGCAACAt                         >  2:1110388/1‑143 (MQ=255)

                                                                                                                             gCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGACAAAACGCTTCTTAAGCGAACTGACCGCCGCCGAAGGTCGTGAACGTTACCTCGGCGAAAAACTCCCTGgcgcc              >  2:1246390/1‑142 (MQ=255)

                                                                                                                              cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                    <  2:4243/104‑1 (MQ=255)

                                                                                                                              cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg                                                    >  1:4243/1‑104 (MQ=255)

                                                                                                                                ccGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaaaa           >  2:368547/1‑143 (MQ=255)

                                                                                                                                 cGAAGAAAAACTCTGGATCCAACAGCGTAACGAGTGTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAc          >  1:872516/1‑143 (MQ=255)

                                                                                                                                  gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGAGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAACTCCCTGGCGCAAAACg         >  2:944234/1‑143 (MQ=255)

                                                                                                                                     gaaAAACTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt      >  1:315867/1‑143 (MQ=255)

                                                                                                                                       aaaaCTCTGGATCCAACAGCGTATCGAGTCTGG‑TT‑GATCTACGTGCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtct    <  1:859955/143‑1 (MQ=255)

                                                                                                                                         aaCTCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCCTGAACGTTACCTCGGCGCCAAATTCCCTGGGGCACAACGCTTCCCg  >  2:725871/1‑143 (MQ=255)

CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGG‑TC‑GCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG  >  NC_000913/759110‑759389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A