Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F21 I1 R1
|
22 |
41.7 |
1602136 |
90.4% |
1448330 |
141.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 97.1%
| 92.8
/ NA
| 36 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (17/17); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACG > NC_000913/759111‑759382
|
cAGGAGACCTTCAACGTCGCTTGATTCGCGAGCGAGACAGAAATCAGGAAACTCGGCGGGCTGCTGGAGGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGGGTATATGCACATTACCAGCACCGAAGAAAAACTCTg < 2:159405/143‑1 (MQ=255)
cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAAc > 1:259915/1‑143 (MQ=255)
ttCAAGGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 2:458131/143‑1 (MQ=255)
cAATGTCGGTTCATTTGCCAGCCGCAAAGAAATCATGAAACTCGGAGAGCTGCTGTAAGCCATCAAGCAATCCTACTTCGGCCCGATTGGTGCCGAGTATATGCACATTTGCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 2:368896/143‑1 (MQ=255)
cAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGAGCCAACAGc > 1:380962/1‑143 (MQ=255)
cATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGATGCCCTCAAGCAAACCTACTGCGGCGCGATTGGTGCCGAGTATAGGCACATTACCAGGAGCGAAGAAAAACTCTGGATGAGACAGCGTGTCGAGGCt > 1:403990/1‑143 (MQ=255)
aTTTGCCAGCGGCAAAGACACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTg < 1:314372/143‑1 (MQ=255)
cggtggCAAAGAAACCATGAAACTCGGCGCGCCGCTGAAAGCCCTCAAGCAAACCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTcgcg < 1:216035/139‑1 (MQ=255)
cAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAACACACTGGATCCAACATCGTATCGAGTCTGGTCGCGCCACtt > 1:541116/1‑143 (MQ=255)
aggcaaCCATGAAACTCGGCGGGCTGCGGGAAGCCCTCAATCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc < 2:348165/139‑1 (MQ=255)
cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAAACTACTGCGGCACGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTGTCGAGACTGGTCGCGCGCCTGTCAATcgcg > 1:244030/1‑143 (MQ=255)
aTGGAACTCGGCGACCTGCGGGAAGCCCTCAAGCTAACCTAATACGGCCCGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGa < 2:476028/143‑1 (MQ=255)
gAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAag > 1:62656/1‑143 (MQ=255)
gagcccggcggGCTGGTGGGGGTGCTCGAGCATACCTACTGCGGCCCGAGTGGTGCGGCGTATATGCACATTACCAGCGCCGAAGAAAAACGCTGGATACAACAGCGGATCGAGGCTGGTCGCGCGACTTTCAATAGCGAaga < 2:516814/138‑1 (MQ=255)
gcGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTAGTCGCGCGACTTTCAATAGCGAAGAGaaa < 2:655119/142‑1 (MQ=255)
cGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTCGTCGCGCGACTGTCAATAGCGAAGAGgaaaaaa > 2:118302/1‑142 (MQ=255)
ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCCAGTCTGGTAGCGCGACTTTCAATAGAGAAGAGAAAAAACGCTTCTTaa > 2:243465/1‑143 (MQ=255)
gAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg > 2:699347/1‑143 (MQ=255)
ccccccaGCAACCCTACCGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATTCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 1:517637/137‑1 (MQ=255)
cccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCCACAGCGTCTCGAGTCTGGTCGCGCGACTTTCCATAGCGAAGAGAAAAAACGCGCCTTAAGCGaa > 1:65101/1‑143 (MQ=255)
cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGGACt > 1:81526/1‑143 (MQ=255)
aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 1:542781/143‑1 (MQ=255)
aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGACGAGAAAAAACGCTTCTTAAGCGAACTGAcc > 1:308084/1‑143 (MQ=255)
aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACcgccgc < 2:259915/143‑1 (MQ=255)
aCTGCGGGCCGATTGGGGCCGAGTATATGCACATTAGCGGGAACGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGaa < 2:353265/143‑1 (MQ=255)
aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCCAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCGGAAGGGCGTGAGCg > 2:258588/1‑143 (MQ=255)
aTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGAGTATCGGGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGCAATGACCGCCGTTGAAGGTGTTGAGCg > 1:330384/1‑143 (MQ=255)
ggTGCCGAGTATATCCACAGTACGAGCCCCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 2:330384/143‑1 (MQ=255)
gTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAAATATGGATACAAGAGGGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGTAGGTCTGGAACGttgc > 1:338597/1‑141 (MQ=255)
cGAGTATATGCACATTACCCGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg < 1:112099/143‑1 (MQ=255)
cGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg > 1:447169/1‑143 (MQ=255)
caTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 2:62656/143‑1 (MQ=255)
ttgccAGCACCGAAGAATAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCt < 2:648342/140‑1 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg > 1:288487/1‑143 (MQ=255)
gaagaaACGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACg < 2:709936/143‑1 (MQ=255)
gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCAGGTCACGCGACTTTCAATAGCCAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGACCGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACg > 2:607945/1‑143 (MQ=255)
|
CAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACG > NC_000913/759111‑759382
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
