Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F104 I0 R1
|
40 |
45.6 |
1767846 |
90.1% |
1592829 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
100% |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 100.0%
| 66.2
/ ‑6.7
| 28 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (15/12); total (16/12) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA > NC_000913/759127‑759380
|
tCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACGC‑GGCGAGCTGCGGGAGGCCCTCAAGCAAACCTACTGCGGCCCGATGGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc < 2:300979/143‑1 (MQ=255)
gTTCATTTGCCAGCGGCAAAGAAACCATGAAACGC‑CGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg > 1:605128/1‑143 (MQ=255)
ttCATGTGCCAGGGGCAAGGAAACCA‑GTAAGTCGGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGGGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGt < 2:760465/143‑1 (MQ=255)
agttGCCACCGTCAACGAACCCATGAAACTC‑GGCGAGCTGCGGGAAGCCCTCCAGCACACCTACTGCGGCCTGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTg < 1:78995/141‑1 (MQ=255)
cAAAGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCCCCGAAGGAAGACTCTGGATCCAACAGCGTACCCAGGCTCGTCGCCCGACtt > 2:511062/1‑143 (MQ=255)
aaGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc > 1:593705/1‑143 (MQ=255)
aaGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGCACCGAGTAGATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGCCGTTc > 2:351928/1‑143 (MQ=255)
ccATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc < 2:654510/143‑1 (MQ=255)
aaaCTC‑GGCGAGCTGCTCGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTCGATCCCACAGCGTCGCCACGCTGGTCGCGCGACTTTCAATAGCGAaga < 1:839159/143‑1 (MQ=255)
c‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCGGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaa < 2:777382/143‑1 (MQ=255)
gacgCCCTCAAGCAAACGTACTGCCGCCCGATTGGTGCTGAGTATATGCACATTACCAGCACCGAAGAAAAGCTCGGAATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 2:405382/140‑1 (MQ=255)
aaGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGCACCCAACAGCGTATCGAGTCTGGACGCGCGACTTTCAATAGCGAAGAGAAAAAACACTTCTTAAGc > 2:527855/1‑143 (MQ=255)
aGCCCTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCg < 2:233365/143‑1 (MQ=255)
cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGGGCACt > 1:438998/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGCAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAGTAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 1:811035/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAAACGCTTCGTACGCGCACTg > 1:660609/1‑143 (MQ=255)
acgCAGACCTCCTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGCCTAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:764101/141‑1 (MQ=255)
aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc > 2:194645/1‑143 (MQ=255)
aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAcc < 1:616895/143‑1 (MQ=255)
aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAACGAACTGAca > 1:470989/1‑142 (MQ=255)
ccTACTGCGGCCCGATTGGTGCCGAGCATATGCACATTACCAGCACTGAAGGAAAACTCTGGATCCAACAGGGTAGCGAGTCTGGTCGCGCGGGTTTCAAAAGCGAAGAGAAACAACGCTGCTTAAGCGAACGGAcggccgag > 1:220970/1‑141 (MQ=255)
cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg > 1:215643/1‑143 (MQ=255)
ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGCCGCGCGACTGTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCCGAAGGCCATGAACGTTAccac > 2:341340/1‑141 (MQ=255)
cgCACATTACCACCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaaaa < 2:501922/142‑1 (MQ=255)
cacaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAAtt > 1:432493/1‑143 (MQ=255)
acacTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTc < 1:851360/143‑1 (MQ=255)
tACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTg > 2:454070/1‑143 (MQ=255)
ccGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTACAATAGCGACGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGGTACCTCGGCGCATACTTCCCTGGAGCataa > 1:106106/1‑143 (MQ=255)
|
TCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTC‑GGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAA > NC_000913/759127‑759380
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
