Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F20 I0 R1
|
35 |
38.1 |
1546666 |
86.7% |
1340959 |
142.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
100% |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 100.0%
| 91.4
/ ‑6.7
| 35 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (19/15); minor base A (0/1); total (19/16) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759117‑759387
|
aCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACCTTACCACCACCGAAGAAAAAATCTGGCTCCa > 2:322739/1‑143 (MQ=255)
cTTCCACGTCGGTTCATTTGCCAGCGGCCAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAAc < 2:478213/143‑1 (MQ=255)
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:196649/143‑1 (MQ=255)
ttCAACGTCGGGTCATTTGCCAGCGGCAACGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 2:572763/143‑1 (MQ=255)
atcagtgTCTGTTCAGTTGTCAGGGGCAAAGAAACTATGAAACTGGGCGAGCTGATGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:693334/137‑1 (MQ=255)
tCAACGTCGTTTAAATAGCCAGACGCAAAGAAACCATGAAACGCGGCGAGCGGCTGGAAGCCCTCAAGCAAACCCACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAg < 1:290007/143‑1 (MQ=255)
cGGTTCATTTGCCAGCGTCAAAGAAACCATGAATCTCGGCGAGCTGCTGGAAGCCCGCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCg < 1:657108/143‑1 (MQ=255)
aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGACTCGGGTCGAGCGACTTTc > 2:346238/1‑143 (MQ=255)
aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAGCTGCACATTACATGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGACTGGTcgcgccagtatc > 2:350777/1‑136 (MQ=255)
aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTAAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGGATCGAGTCTGGTCGCGCGAcgggc > 2:417768/1‑139 (MQ=255)
aaGAAACCATGAAACTCGGCGAGCTGCTCGACGTCGTTAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTGCCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCCAGCCTCGTCGCGCGACTGTc > 2:372246/1‑143 (MQ=255)
ccATGAAACTCTGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc < 1:191921/143‑1 (MQ=255)
cATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGGTTGGTGCCGCGTAGCTGCACCTTACCAGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCGGGTCGCGCGACTTTCAATAGca > 1:157931/1‑142 (MQ=255)
aCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACATACTGCGGACCGATTGGTGCCGAGTATATGCACATTAACAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATAGAGTCTGGTCGCGCAACATTCAATAGCGAAgaga > 2:211191/1‑143 (MQ=255)
acgccAAGCAAACCTACTGCGGCCCGATTGGGGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCCGGTCGCGCGACTTTCAAGAGCGAAGAGAAAAAACGCTTCTTAAGCGaa < 1:321034/139‑1 (MQ=255)
ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCATATCGAGTCTGGTCGCGCGACCTTCAATAGCGAAGAGCAAAAACACTTCTTACGCGAAc > 2:408328/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 1:84272/1‑143 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTg > 1:443368/1‑143 (MQ=255)
gCACACCTACTCCGGCCCGATTGGTGCCGAGTATCTGGACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTGTCGAGTCTGGTCTCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAccg < 2:324856/143‑1 (MQ=255)
aaCCTACTGTGGCTCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACGTTCAATAGCGAAGATAAAAAACGCTTCTTAAGCGAACTGAccgccg < 2:319296/143‑1 (MQ=255)
aCCTTCTGCTGCCTGATTGGTGCCGAGTATATGAACAAAACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACcgccgc < 1:364921/143‑1 (MQ=255)
ccTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACAAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGACTGGCCGCGCGACTCTCAATAGCGCAGCGAAAAAAGGCTCCTGAAGCGAACTGACcgccgcc > 2:763810/1‑142 (MQ=255)
cTGCGGCCCGGTTGGTGCTGAGTATATGCTTATTACCAGCTCAGAAGAAAAACACTGGAGCCAACAGCGTATCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:212766/143‑1 (MQ=255)
atGCGGCTCGATTGGTGCCGAGTACATGCACCTGACCCGCACCGAAGAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAg < 1:308417/142‑1 (MQ=255)
tGCGGCCCGATTGGTGCCGAGTATATGAACATTACCAGCACCGAAGAAAAACTCTGGATCTAAAAGCGGATCGAGTCTGGTAGCGCGACTTTAGAGAGGGAAGAGAACACACGCAACTTAAGCCAACCGAGCGCGGCTGAAgg > 2:15021/1‑143 (MQ=255)
ggccGATTGGTGCCGAGTATATGCACATTACCAGCACAGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 2:141197/141‑1 (MQ=255)
ggTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATACCGAAGAGAAACAACGCTTCTTAAGCAAACTAACCGAAGCTGAAGGTCTTGAACGTTa > 1:683623/1‑143 (MQ=255)
ggTGCCGAGCATATGCACACAACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTa < 1:559629/143‑1 (MQ=255)
tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAcc > 1:323249/1‑143 (MQ=255)
ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATAGAGTCTGGTAGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGAGAACTGACAGACGCTGAAGGTATTGAAGGTTAcata > 1:450567/1‑140 (MQ=255)
cGAGTACATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg < 1:364656/143‑1 (MQ=255)
tataTGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGGCTTGAACGTTACCTCGgcgc > 2:510373/1‑143 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCCCGGCGCACACTTCCCTGccg > 1:772338/1‑143 (MQ=255)
gCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAATCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACATTACCCCGGCGCAAAATTcccgagcccc > 2:467123/1‑136 (MQ=255)
aaaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACAGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATCGCCTGGAGCAAGACgagtcg > 2:77481/1‑138 (MQ=255)
|
ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCT > NC_000913/759117‑759387
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
