Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F23 I0 R1
|
55 |
48.2 |
1840046 |
90.8% |
1670761 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
100% |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 100.0%
| 83.9
/ ‑6.7
| 34 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (17/14); total (20/14) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388
|
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCTAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATCAGCAGGACGGAAGGAAAACACt > 1:460170/1‑143 (MQ=255)
ccAGGAGACCTTCAACGTCGGTTCATGTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 1:167/143‑1 (MQ=255)
aGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGTAGCCGCGTATATGCACATTACCAACCCCGCAGACAAACTCTgg > 1:77748/1‑143 (MQ=255)
ccTTCACCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCaa < 2:690742/143‑1 (MQ=255)
cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCCCATTACCAGCACCGCAGAAAAACTCTGGAACCAAc > 2:490942/1‑143 (MQ=255)
cacccgcGGTTTACTTGCCAGCGGCAAAGAAGCCACGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 2:472940/137‑1 (MQ=255)
cGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGCTGACGCGTATAGGCACATTACCACCGCCGAAGACAAACCCTGGACCCAACcgcgcaccg > 2:550554/1‑138 (MQ=255)
ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGa < 1:660882/143‑1 (MQ=255)
cATTTGCCAGCGGCAAAGAAACCATGGAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCt < 2:827068/143‑1 (MQ=255)
gCGGGAAAGAGGCCAGGAAACGCGGCGGGCTGCTGGAAGGCCTCAAGCAAACCTACGGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcgcg < 2:767558/143‑1 (MQ=255)
cGGCAAAGAAACCATGAAACTCGGCGGGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATCTGCACATTACCAGCCCCGAAGAACAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGa > 2:874668/1‑143 (MQ=255)
aaaGAAACCATGAAACTCGGCGGGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt > 1:253109/1‑143 (MQ=255)
aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAt < 1:99141/143‑1 (MQ=255)
aTGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGGCTTTCAATGGCGa > 1:494868/1‑143 (MQ=255)
tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTCCTGCGGCTCGTTTTGTTCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa < 1:385390/143‑1 (MQ=255)
tGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCgca > 1:530452/1‑141 (MQ=255)
aaCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAagag > 1:278701/1‑143 (MQ=255)
ccTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACTGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAAc > 1:461506/1‑143 (MQ=255)
cTCAAGCACACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGCAGAAAAACTCTTGATCCAACCGCGTATCGGGTCTGGTCGCGCGACGGTGAATAGCGAAGAGAACAAACGCCTCTGAAGGGAACt > 1:19475/1‑143 (MQ=255)
cTCAAGCAAACCAACTGCGGCCCGATTGGTGCCTGGTATATGCACATTACCAGCACCGAAGAAAAGCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCGATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt < 2:543582/143‑1 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTg > 1:793549/1‑143 (MQ=255)
cGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAACAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAGTCGCGAAGCGAAAAAGCGCTTCTTAAGCGAACTGACCGCCGCTGAGGGTc > 1:81332/1‑143 (MQ=255)
ccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCttca > 2:668073/1‑141 (MQ=255)
cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGaa < 2:278701/143‑1 (MQ=255)
caTTACCAGCACCGAACAAACACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTcc < 1:41539/143‑1 (MQ=255)
ttACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCt > 1:63543/1‑143 (MQ=255)
caCCAGCAGCGAAGAAAAGCTTTGGATCCAACAGCGTATCGAGTCTTGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTg < 2:829217/142‑1 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGTAAATACTCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg < 1:343592/143‑1 (MQ=255)
cAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTGTCAATAGCGAAGAGAGAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGCACGTTAGCTCGGCGCAAAATTCACTGgcg > 2:809694/1‑143 (MQ=255)
cGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAc > 1:847325/1‑143 (MQ=255)
cGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGCCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAc > 2:255186/1‑143 (MQ=255)
aagaaAAACTCTGGATCCAACAGCGTAACGAGCCTCAACGCACGACATTCAATAGCGAAGAGAAAAACCGCTTCTGAAGCGAACTGACCGCCGCTGAAGGTCTTTAACGTTACCTCGGCGCAAAATCCCCTCGCGCaagccgc > 2:233742/1‑138 (MQ=255)
agaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATTGCGAAGAGTAAAATCTCTTCTTATGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCt < 1:497170/143‑1 (MQ=255)
gaaAAACTCTGGAACCAACAGCTTATCAAGCCTGGTCGCGCGACTTTAAAAAAAAAAAAAAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCtt < 2:676636/143‑1 (MQ=255)
aaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTGAAGCGAACTGACCGCCGCTGAAGGCCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAACACGCTtctc > 2:2244/1‑143 (MQ=255)
|
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
