Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A19 F91 I1 R1
|
26 |
61.7 |
2321240 |
90.8% |
2107685 |
142.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
759,250 |
G→T |
R182L (CGC→CTC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 759,250 | 0 | G | T | 92.0%
| 115.1
/ ‑5.3
| 51 | R182L (CGC→CTC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (15/31); total (19/32) |
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388
|
ccAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAActct < 1:981290/143‑1 (MQ=255)
agaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGCGACGAGCATATGCACATCACCAGCACCGAAGAAAAACGATGAATc > 2:806764/1‑143 (MQ=255)
gaCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAACCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATcc < 2:47942/143‑1 (MQ=255)
cgtcgaCGGCGGTTCGTTTGCCGGCGGCAGAGAAACCATGGAACTCGGCGAGCTGCTGGGAGCCCTCAATCATGCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCTGCACCGAAGAAAAACTCTGGATCCAAc < 2:1050058/138‑1 (MQ=255)
cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGGATATGCACATTACCAGGACCGAAGAAAAACGCTGGATCCAAc > 2:736066/1‑143 (MQ=255)
ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCCTGCCCCTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:11219/143‑1 (MQ=255)
gtCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa < 1:506766/142‑1 (MQ=255)
ggagggcGGTTGATTTGCGAGCGGCAAAGAGACGATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAGCCTCCTGCGGCCCGATTGGTGCCGTGTATCTGCGTCGTTCCCGCGCCGAAGAAAAACTCTGGATCCAACAGc < 2:356707/137‑1 (MQ=255)
caccgccGGTGCATTTGCCAGCGGCACAGAAAACATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGc < 2:41834/137‑1 (MQ=255)
aCGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGt < 2:847775/143‑1 (MQ=255)
gTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTAAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACCTTACCAGCACCGCAGGACAACTCTGGATCCAACAGCGtct > 1:542472/1‑141 (MQ=255)
cGGTTCATTTGCCAGCGGCGAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCg < 1:596335/143‑1 (MQ=255)
gTTCATTCGCCAGCGGCAAAGAAACCATTAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAg < 2:616446/143‑1 (MQ=255)
ttCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCGCATTACCAGCCCCGAAGCAAAGCTCTGCATCCAACGACGTATCgcgc > 2:39676/1‑140 (MQ=255)
tttGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCttg > 1:509210/1‑141 (MQ=255)
ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATAGGTGCCGCGAATATGCACATTACCAGCACCGAAGAAAGACGATGCATCCAACAGCGTATAGAGTCTGGTcac > 2:1054160/1‑141 (MQ=255)
ccAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACATACTGCGGCCAGATTGGTGCCGAGTCTACGCACATTACCAGCACCGAAGAAAAACTCAAGATCCAACAGCGTATCGAGTCTGGTcgc > 2:505795/1‑143 (MQ=255)
ggCAAAGAAACCATGAAACGCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGAc < 1:205100/143‑1 (MQ=255)
gCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTAGATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTcgcgcgccg > 1:799460/1‑140 (MQ=255)
gccGGCGTGCTGCTGGCCGCACTGTAGCAAACCTACCGCGGCCCGATNGGTGCTGAGTACAGCCACATCACCAGCACCGAAGACAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaa < 2:73001/141‑1 (MQ=255)
tCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaa > 1:895300/1‑143 (MQ=255)
gCGAGCGGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTACCGAGCCTGATCACGCGACTCTCAATAGCGACGAGaaaaaa > 2:471748/1‑143 (MQ=255)
ccgttgtaaGTCCGCAATCAAACCTTCTGCGGCCCGATTGGTGTCGAGTATATGTACATTATCAGCACTGAGGTAAACCTCTGTATCAAATAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGctt < 2:33849/136‑1 (MQ=255)
gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCTATTGGTGCCGAGTATATGCATATAACCAGCAAAGCCAATGTACTCTGGATCCAACAGCGTATCGACTCTGGTCGCGCGCCTTTCCATAGCGAAGAGCAAAACCGcttct > 2:822647/1‑143 (MQ=255)
gctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCCGCACCGAAGAAAAACTCTGGATCCAACAGCGCATCGAGTCTGGCCGCGCCACTCTCAATACCGAAGCGAAAAAACGcttct > 2:534095/1‑143 (MQ=255)
cgggAAGCCCGCAACCAAACCTACTGCGGCTGGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGcttctt < 1:374602/141‑1 (MQ=255)
ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 1:1034866/143‑1 (MQ=255)
ggAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTaa < 1:1078667/143‑1 (MQ=255)
gAAGCCCTCAAACAACCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAg < 1:1086352/143‑1 (MQ=255)
aaGCCCTCAAGCAAACCTCCCGCCGCCCGAGTGGTGCCGAGTATCTGGGTGTGACCACCACCAAAGATAAGCTCGTTATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGc < 2:1126839/143‑1 (MQ=255)
cTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACt < 1:872298/143‑1 (MQ=255)
tCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACCCTGGACCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCAAAcct > 2:495469/1‑141 (MQ=255)
aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATAGGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc < 2:662608/143‑1 (MQ=255)
gCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGt < 1:948684/143‑1 (MQ=255)
cGGCGCTATCGGTGCCGAATATACGCACAGTACCAGCACCGAAGAAAAACTCTTGATCCAACAGCGTATCGAGTCTGGTCGCGCCACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTc < 1:912399/143‑1 (MQ=255)
ggCCCGATTGGTGCCGAGGATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCt < 1:1136210/143‑1 (MQ=255)
acccGATTGCCGCCGAGTATACGCACATTCCCAGCACCGAATAAAAACTCTGGATCCAACAGCGCATCCAGTCTCGACGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCtt < 1:163657/142‑1 (MQ=255)
cccGATTGGGGACGAGTAACTGCACAGTACCGCCACCGGAGAAAAACTCTGGAGCCAACAGCGTATCGAGTCTGGTCGCGCGACTATCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg < 2:1142804/143‑1 (MQ=255)
caTTGGTTCCGAGTATATGCACATTCCCAGCACCGAAGAAAATCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc < 2:596377/142‑1 (MQ=255)
tGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGtt > 1:454367/1‑143 (MQ=255)
gTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTAc < 1:1054160/143‑1 (MQ=255)
ccGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTc < 1:622469/143‑1 (MQ=255)
gcgttgtatGCACAGTACCAGCGTCGGGGGAACACTCCGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAAGAGCGATGAGAAAAAACGCTTCTTAAGCGAACTGGCCGCCGCTGAAGGTCTTGAACGTTACCTCg < 2:185693/137‑1 (MQ=255)
gAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCgg > 2:43866/1‑143 (MQ=255)
agcatatGCACACTACCCGCCCCGAAGAAAAGCTCTGGACCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAAGAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGgc < 1:55130/140‑1 (MQ=255)
atatGCGCAGTACCGGCCGCGCAGAAAGACTCTGGAGCCAACAGCTTATCGAGTCTGGTCGCGCGACTTTCAATGGCGATGAGAAAACACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCa < 2:463434/143‑1 (MQ=255)
acaTTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGACGCAAAATTc > 2:487347/1‑143 (MQ=255)
cccgCACCGACGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAACAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc < 1:54827/140‑1 (MQ=255)
cAGCACCGAAGAAAGACTCTGGATCGAAAAGCGTATCGAGTCTGGTCTCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgcg < 2:1063798/143‑1 (MQ=255)
cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa > 2:403577/1‑143 (MQ=255)
aagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGGCGCAAAACGc > 2:54608/1‑143 (MQ=255)
aaaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCACTAGCGAAGAGAAAAACCGCTTCTTCAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTtccc > 2:695332/1‑141 (MQ=255)
|
CCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTC > NC_000913/759110‑759388
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
