Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F44 I0 R1	115	55.9	2109214	91.7%	1934149	142.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	759,250	G→T	100%	R182L (CGC→CTC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,250	0	G	T	100.0%	106.9 / NA	40	R182L (CGC→CTC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/26); total (14/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG  >  NC_000913/759117‑759389

aCCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATCTGCACATTACCAGCACCGAAGAAACACTCTGGATCCa                                                                                                                                    >  1:779921/1‑143 (MQ=255)

   ttCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACa                                                                                                                                 >  1:537817/1‑143 (MQ=255)

    tCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCCCCGCAGACAAACTCGGGATCCAGCac                                                                                                                                >  2:701202/1‑142 (MQ=255)

          tCGGCTCATTGGCCTGTCGCAAAGAAACCATGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATc                                                                                                                          <  2:779353/143‑1 (MQ=255)

            ggTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGa                                                                                                                        <  1:735148/143‑1 (MQ=255)

                cATTTGCCAGCGGCAAAGAAAACATGAAACCCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCt                                                                                                                    <  1:25296/143‑1 (MQ=255)

                              aaaGAAACCATGAACGTCGGCGAGCTGCTGGAAGCCATCAAGCAAACCTACTGCGGCCCGACTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt                                                                                                      <  2:875165/143‑1 (MQ=255)

                              aaaGAAACCAGGGAGCTCGACGCGCTGCTGGAAGCCCTCAAGCAAATCTCCGGCGGCCCGAGTGGTGCCGAGTATATGCACATTAGCAGCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACttt                                                                                                      <  2:177322/143‑1 (MQ=255)

                               aaGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTc                                                                                                     <  1:543063/143‑1 (MQ=255)

                                aGAAGCCATGAAACTCGGCGAGCGGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACAGTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCa                                                                                                    <  2:392508/143‑1 (MQ=255)

                                aGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGCGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACtttaa                                                                                                    >  1:1014632/1‑141 (MQ=255)

                                  aaaCCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCact                                                                                                  >  1:434143/1‑141 (MQ=255)

                                     ccATGAACCGCGCCGAGCCGCTGGAGGCCCCCAAGCAAACCTACTGCCGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGc                                                                                               <  1:895863/143‑1 (MQ=255)

                                                 gCGTGCGGCTAGACGCCCTCAAGCAAACCCACAGCGGCCCGATTGGTGCCGAGTATATGCACATTACCCACACCGAAGAAAAACTCTGGATCCAACAGCGTATCAAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGaaaaaa                                                                                   <  2:227719/143‑1 (MQ=255)

                                                     gctgctGGAAGCCCTCAAGCAAACCTACTGCGGCCCGCTTGGCGCCGAGTATATGCACATTACCAGCACCGAAGACAAACTCTGGCTCCAACAGCGTCTCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAACAAAGGca                                                                               >  1:977659/1‑142 (MQ=255)

                                                         ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa                                                                                            <  1:649792/126‑1 (MQ=255)

                                                         ctgGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGaa                                                                                            >  2:649792/1‑126 (MQ=255)

                                                                  cTCAAGCAAACCTAATGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGGAGAGAAAAAACGCTTCTTAAGCGAACt                                                                  >  1:558312/1‑143 (MQ=255)

                                                                    cAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGNAAAAACGCTTCTTAAGCGCACTgg                                                                <  1:161222/143‑2 (MQ=255)

                                                                     tattaaaCCTACTGCGGCCCGAATGGGGCCGAGTATACGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc                                                               <  1:900145/139‑1 (MQ=255)

                                                                     aaGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTAGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGAc                                                               >  1:238421/1‑143 (MQ=255)

                                                                            ccTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGCCTGGTCGCGCGACTTCCAATAGCGAAGAGAACAAACGCTTCTTAAGCCAACTGACCGCCGCt                                                        >  2:81202/1‑143 (MQ=255)

                                                                                 tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGCAAAAACGCTTCTTAAGCGGACGGGCCGCCGCGGAAgg                                                   >  1:755536/1‑143 (MQ=255)

                                                                                    ggCCCGATTGATGCCGAGGATATGCACATGACCAGCAGCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCt                                                <  2:239295/143‑1 (MQ=255)

                                                                                      cccGATTGGTGCCGGGTAGATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                              <  2:548592/143‑1 (MQ=255)

                                                                                      cccGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                              <  2:177874/143‑1 (MQ=255)

                                                                                      cccGAGTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                              <  2:133558/143‑1 (MQ=255)

                                                                                      cccGACTGGTCCCGAGTAGATCGAAATCACCAGCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGTTCGCGCTACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTg                                              <  2:648148/143‑1 (MQ=255)

                                                                                        cGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCCGGTCGCGCGACTTTCAATAGCGAAGAGAAAAACCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCGTGaa                                            >  2:932745/1‑143 (MQ=255)

                                                                                         taTTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAACCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAAc                                           <  2:295604/142‑1 (MQ=255)

                                                                                            tGGTGCCGGGTATATGCACATTACCAGCACCGAAGAAAGACTCTTGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAACCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGtt                                        <  2:89708/143‑1 (MQ=255)

                                                                                            tGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGGCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGACCTGACCGCCGCTGAAGGTCTTGAACGtt                                        >  2:1005920/1‑143 (MQ=255)

                                                                                                  cGAGTATCTGCACATTACCAGCATCGAAGAAATACTCTGGATCTAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCg                                  <  2:81825/143‑1 (MQ=255)

                                                                                                        tatGCACATTACCAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCaa                            <  1:82700/143‑1 (MQ=255)

                                                                                                                   ccAGCACCGAAGAAAAACTCTGGCTCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc                 <  1:643019/143‑1 (MQ=255)

                                                                                                                   ccAGCACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGgc                 <  1:645149/143‑1 (MQ=255)

                                                                                                                       cACCGAAGAAAAACTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCaa             <  2:434143/143‑1 (MQ=255)

                                                                                                                           gaagaaAAACTCTGGATCCAACAGCGTATCGAGTCTGGGCCCGCGACTTTCGATAACAAACATAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACg         <  1:485795/143‑1 (MQ=255)

                                                                                                                             agaaAAACTCTGGATCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTTAGCGAACTGACCGTCGCTGAAGGTCTTGAACGTTACCTCTGCGCAAAATTCCCTGGCGCAAAACGCt       <  2:275233/143‑1 (MQ=255)

                                                                                                                                  aaCTCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGATGCTTGACCGGACTGACGGCCGCAGAAGGGCTTGAACGGTACCTCGGAGCAAAATTACCTGGGGCGCAACggtacaag  >  2:574589/1‑136 (MQ=255)

ACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACCATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG  >  NC_000913/759117‑759389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A