breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 9,907 A→C 14.0% intergenic (+14/+21) mog → / ← satP molybdopterin adenylyltransferase/acetate/succinate:H(+) symporter
RA 125,387 C→T 100% Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 639,685 Δ1 bp 14.7% intergenic (+177/‑68) ahpC → / → ahpF alkyl hydroperoxide reductase, AhpC component/alkyl hydroperoxide reductase, AhpF component
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 759,250 G→T 100% R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 786,458 Δ1 bp 100% coding (826/1053 nt) aroG → 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
RA 1,068,231 A→G 11.7% S51G (AGT→GGT)  ymdF → conserved protein YmdF
RA 1,098,045 G→C 9.8% G54R (GGG→CGG)  ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,098,054 C→T 10.9% L57F (CTT→TTT)  ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,111,164 G→T 32.1% R101L (CGT→CTT)  opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,406,853 C→A 11.3% A315S (GCC→TCC)  dgcM ← diguanylate cyclase DgcM
RA 1,764,427 G→A 12.4% intergenic (‑41/+286) sufA ← / ← rydB iron‑sulfur cluster insertion protein SufA/small regulatory RNA RydB
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,321,822 T→C 12.3% F594L (TTC→CTC)  atoS → sensory histidine kinase AtoS
RA 2,399,516 C→A 16.9% R217L (CGT→CTT)  nuoG ← NADH:quinone oxidoreductase subunit G
JC 2,399,711 Δ123 bp 11.6% coding (333‑455/2727 nt) nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,134,229 T→C 8.2% Y33Y (TAT→TAC yghT → putative ATP‑binding protein YghT
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,051,262 A→C 8.4% noncoding (227/245 nt) csrC → small regulatory RNA CsrC
RA 4,159,147 C→G 16.9% R220G (CGC→GGC)  oxyR → DNA‑binding transcriptional dual regulator OxyR
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,296,060 C→T 30.1% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,391,920 G→C 15.6% G106A (GGT→GCT)  orn → oligoribonuclease

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366265 366398 134 12 [11] [11] 12 [lacZ] [lacZ]
* * ÷ NC_000913 3423763–3424234 3424536–3424238 5–774 12 [11] [11] 13 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 315520NA (NA)3 (0.080) 3/250 NT NA noncoding (292/1255 nt) IS3 repeat region
?NC_000913 = 315537 NA (NA)noncoding (309/1255 nt) IS3 repeat region
* ? NC_000913 = 392272NA (NA)5 (0.140) 5/258 NT NA noncoding (695/1258 nt) IS3 repeat region
?NC_000913 = 392297 NA (NA)noncoding (670/1258 nt) IS3 repeat region
* ? NC_000913 566777 =NA (NA)1798 (48.410) 253/262 NT 98.3% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 32 (0.860)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 57247132 (0.860)8 (0.290)
+34 bp
6/196 NT 30.2% coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
?NC_000913 = 2170235 18 (0.480)noncoding (65/1258 nt) IS3 repeat region
* ? NC_000913 1080908 =40 (1.070)4 (0.120) 3/240 NT 9.7% intergenic (+95/‑448) putP/efeU proline:Na(+) symporter/inactive ferrous iron permease EfeU, N‑terminal fragment
?NC_000913 2229207 = 38 (1.120)intergenic (+142/+231) yohP/dusC putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
* ? NC_000913 1207790 =5 (0.130)24 (0.730) 20/232 NT 75.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 11 (0.330)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078054 (0.110)16 (0.490) 15/232 NT 68.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 11 (0.330)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1437201 =28 (0.750)3 (0.080) 3/250 NT 10.3% noncoding (81/109 nt) micC small regulatory RNA MicC
?NC_000913 1437225 = 26 (0.730)noncoding (105/109 nt) micC small regulatory RNA MicC
* ? NC_000913 = 4254000NA (NA)4 (0.110) 4/250 NT NA noncoding (32/34 nt) other REP317c
?NC_000913 = 4254030 NA (NA)noncoding (2/34 nt) other REP317c
* ? NC_000913 4628744 =NA (NA)3 (0.080) 3/250 NT NA noncoding (1/37 nt) other REP354b
?NC_000913 4628775 = NA (NA)noncoding (32/37 nt) other REP354b