breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 125,083 C→T 37.6% Y689Y (TAC→TAT aceE → pyruvate dehydrogenase E1 component
RA 125,387 C→T 100% Q791* (CAG→TAG)  aceE → pyruvate dehydrogenase E1 component
MC JC 257,908 Δ776 bp 100% insB9[crl] insB9, insA9, [crl]
RA 371,064 C→G 9.4% A263G (GCC→GGC)  mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase
RA 371,067 T→A 9.7% I264N (ATT→AAT)  mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase
RA 371,070 C→G 8.0% A265G (GCC→GGC)  mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase
RA 496,171 C→T 7.0% A351V (GCG→GTG)  htpG → chaperone protein HtpG
RA 657,531 T→G 7.1% intergenic (+30/+24) cspE → / ← crcB transcription antiterminator and regulator of RNA stability/F(‑) channel
RA 657,533 A→T 6.9% intergenic (+32/+22) cspE → / ← crcB transcription antiterminator and regulator of RNA stability/F(‑) channel
RA 696,328 A→C 12.9% intergenic (+52/+102) ubiF → / ← glnX 2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase/tRNA‑Gln
RA 696,470 C→T 100% noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 710,170 C→T 9.6% intergenic (+54/+30) chiQ → / ← fur lipoprotein ChiQ/DNA‑binding transcriptional dual regulator Fur
RA 710,173 A→G 12.0% intergenic (+57/+27) chiQ → / ← fur lipoprotein ChiQ/DNA‑binding transcriptional dual regulator Fur
RA 710,595 G→T 100% P18T (CCT→ACT)  fur ← DNA‑binding transcriptional dual regulator Fur
RA 733,768 G→A 5.7% V1396M (GTG→ATG)  rhsC → rhs element protein RhsC
RA 759,250 G→T 100% R182L (CGC→CTC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 1,018,983 A→T 9.5% intergenic (+46/+30) matP → / ← ompA macrodomain Ter protein/outer membrane porin A
RA 1,196,220 C→T 100% H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T 100% T370T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% insH21 insH21
RA 1,498,892 A→T 8.2% intergenic (+17/‑46) ydcH → / → rimL protein YdcH/ribosomal‑protein‑L12‑serine acetyltransferase
RA 1,498,894 C→G 8.2% intergenic (+19/‑44) ydcH → / → rimL protein YdcH/ribosomal‑protein‑L12‑serine acetyltransferase
RA 1,498,896 A→T 8.3% intergenic (+21/‑42) ydcH → / → rimL protein YdcH/ribosomal‑protein‑L12‑serine acetyltransferase
RA 1,563,410 C→G 9.9% G775A (GGA→GCA)  dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,778,367 C→G 8.0% intergenic (+20/+23) ydiO → / ← ydiP putative acyl‑CoA dehydrogenase/putative DNA‑binding transcriptional regulator YdiP
RA 1,850,715 Δ1 bp 11.4% intergenic (+22/‑145) sppA → / → ansA protease IV, a signal peptide peptidase/L‑asparaginase 1
RA 1,850,719:1 +T 12.5% intergenic (+26/‑141) sppA → / → ansA protease IV, a signal peptide peptidase/L‑asparaginase 1
RA 1,864,758 G→A 9.9% intergenic (+24/+24) yeaD → / ← yeaE putative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE
RA 1,864,763 A→T 9.5% intergenic (+29/+19) yeaD → / ← yeaE putative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE
RA 1,864,768 T→C 11.2% intergenic (+34/+14) yeaD → / ← yeaE putative aldose 1‑epimerase YeaD/methylglyoxal reductase YeaE
MC JC 1,978,503 Δ776 bp 100% insB‑5insA‑5 insB‑5, insA‑5
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA‑5 ← / → uspC IS1 protein InsA/universal stress protein C
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,173,359 Δ1 bp 11.9% pseudogene (920/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,173,361 Δ2 bp 100% pseudogene (917‑918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,311,616 C→T 7.0% intergenic (‑82/+30) ftp ← / ← ompC FAD:protein FMN transferase/outer membrane porin C
RA 2,311,619 A→G 8.1% intergenic (‑85/+27) ftp ← / ← ompC FAD:protein FMN transferase/outer membrane porin C
RA 2,402,140 G→A 44.6% P569L (CCG→CTG)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,403,813 C→T 10.5% W11* (TGG→TGA nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,414,689 C→G 9.5% intergenic (+17/‑58) ackA → / → pta acetate kinase/phosphate acetyltransferase
RA 2,561,205 A→T 9.9% intergenic (+307/‑163) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein YffL/CPZ‑55 prophage; uncharacterized protein YffM
RA 2,561,206 A→T 9.1% intergenic (+308/‑162) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein YffL/CPZ‑55 prophage; uncharacterized protein YffM
RA 2,641,353 T→A 49.3% E151V (GAA→GTA) ‡ ispG ← (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin)
RA 2,641,354 C→A 50.5% E151* (GAA→TAA) ‡ ispG ← (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin)
RA 2,661,969 C→G 100% V55L (GTT→CTT)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,761,620 T→G 7.2% K641Q (AAG→CAG)  abpB ← CP4‑57 prophage; putative helicase YfjK
RA 2,761,623 C→A 6.9% V640L (GTA→TTA)  abpB ← CP4‑57 prophage; putative helicase YfjK
RA 3,041,632 T→A 100% V107E (GTG→GAG)  ygfZ → folate‑binding protein
RA 3,259,122 T→G 8.6% E176D (GAA→GAC) ‡ tdcG ← L‑serine deaminase III
RA 3,259,124 C→G 8.1% E176Q (GAA→CAA) ‡ tdcG ← L‑serine deaminase III
RA 3,259,126 C→A 9.7% G175V (GGT→GTT)  tdcG ← L‑serine deaminase III
RA 3,271,825 T→A 8.7% intergenic (‑55/+42) garK ← / ← garR glycerate 2‑kinase 1/tartronate semialdehyde reductase
RA 3,560,455:1 +G 100% pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,637,645:1 +T 100% coding (4/1500 nt) pitA → metal phosphate:H(+) symporter PitA
RA 3,720,802 A→C 8.8% T41T (ACA→ACC insJ → insertion element IS150 protein InsA
RA 3,798,666 A→T 12.4% L216* (TTA→TAA)  waaU ← putative ADP‑heptose:LPS heptosyltransferase 4
MC JC 3,815,859 Δ82 bp 100% [rph] [rph]
RA 3,856,594 A→C 10.6% K60N (AAA→AAC yidI → putative inner membrane protein
RA 3,980,726 G→A 7.9% intergenic (+30/‑161) rffM → / → yifK UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase/putative transporter YifK
JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,019,665 Δ1 bp 25.6% coding (42/606 nt) ubiJ → ubiquinone biosynthesis accessory factor UbiJ
RA 4,056,531 A→G 6.1% intergenic (‑192/+94) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,532 A→C 6.1% intergenic (‑193/+93) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,539 T→A 6.0% intergenic (‑200/+86) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,540 T→A 6.2% intergenic (‑201/+85) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,547 G→T 6.9% intergenic (‑208/+78) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,056,548 C→T 5.8% intergenic (‑209/+77) glnL ← / ← glnA sensory histidine kinase NtrB/glutamine synthetase
RA 4,100,573 A→G 13.7% intergenic (‑48/‑237) rhaT ← / → sodA rhamnose/lyxose:H(+) symporter/superoxide dismutase (Mn)
RA 4,125,097 C→T 100% E572K (GAG→AAG)  priA ← primosome factor N'
RA 4,159,126 G→A 12.5% A213T (GCG→ACG)  oxyR → DNA‑binding transcriptional dual regulator OxyR
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase subunit beta
RA 4,196,268 Δ1 bp 10.8% intergenic (‑169/+64) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,196,269 Δ1 bp 10.8% intergenic (‑170/+63) thiC ← / ← rsd phosphomethylpyrimidine synthase/regulator of sigma D
RA 4,296,060 C→T 29.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,294 A→T 12.3% L169Q (CTG→CAG)  alsK ← D‑allose kinase
RA 4,307,298 G→A 14.2% P168S (CCC→TCC)  alsK ← D‑allose kinase
RA 4,335,654 T→A 7.6% intergenic (‑64/+40) eptA ← / ← adiC phosphoethanolamine transferase EptA/arginine:agmatine antiporter
RA 4,335,655 T→A 9.5% intergenic (‑65/+39) eptA ← / ← adiC phosphoethanolamine transferase EptA/arginine:agmatine antiporter
RA 4,470,951 A→C 11.9% intergenic (‑38/+35) ridA ← / ← pyrI enamine/imine deaminase, redox regulated chaperone/aspartate carbamoyltransferase, PyrI subunit
RA 4,470,952 T→C 10.2% intergenic (‑39/+34) ridA ← / ← pyrI enamine/imine deaminase, redox regulated chaperone/aspartate carbamoyltransferase, PyrI subunit
RA 4,470,957 G→A 11.5% intergenic (‑44/+29) ridA ← / ← pyrI enamine/imine deaminase, redox regulated chaperone/aspartate carbamoyltransferase, PyrI subunit
RA 4,470,958 G→T 11.2% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, redox regulated chaperone/aspartate carbamoyltransferase, PyrI subunit
RA 4,472,437 T→C 8.1% N33D (AAT→GAT)  pyrL ← pyrBI operon leader peptide
RA 4,481,820 G→A 13.2% A673V (GCG→GTG)  valS ← valine‑‑tRNA ligase
RA 4,532,534 G→C 10.0% A217G (GCC→GGC)  yjhP ← KpLE2 phage‑like element; putative methyltransferase YjhP

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196245 1211455 15211 21 [18] [19] 21 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2066277–2066633 2066633 1–357 20 [18] [19] 21 [insH‑6] [insH‑6]
* * ÷ NC_000913 3423729–3424530 3424530 1–802 20 [19] [19] 21 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 22874427 (0.570)13 (0.290) 9/252 NT 33.5% intergenic (+82/‑12) rrlH/rrfH 23S ribosomal RNA/5S ribosomal RNA
?NC_000913 2726224 = NA (NA)intergenic (‑36/+57) rrfG/rrlG 5S ribosomal RNA/23S ribosomal RNA
* ? NC_000913 = 315642NA (NA)8 (0.170) 4/260 NT NA noncoding (414/1255 nt) IS3 repeat region
?NC_000913 = 315712 NA (NA)noncoding (484/1255 nt) IS3 repeat region
* ? NC_000913 315642 =NA (NA)7 (0.150) 7/254 NT NA noncoding (414/1255 nt) IS3 repeat region
?NC_000913 315671 = NA (NA)noncoding (443/1255 nt) IS3 repeat region
* ? NC_000913 = 315689NA (NA)3 (0.060) 3/258 NT NA noncoding (461/1255 nt) IS3 repeat region
?NC_000913 = 315751 NA (NA)noncoding (523/1255 nt) IS3 repeat region
* ? NC_000913 315843 =NA (NA)6 (0.130) 5/258 NT NA noncoding (615/1255 nt) IS3 repeat region
?NC_000913 315886 = NA (NA)noncoding (658/1255 nt) IS3 repeat region
* ? NC_000913 342552 =56 (1.180)3 (0.070) 3/252 NT 5.4% intergenic (+45/‑332) yahJ/yahK putative deaminase with metallo‑dependent hydrolase domain/aldehyde reductase, NADPH‑dependent
?NC_000913 342569 = 52 (1.150)intergenic (+62/‑315) yahJ/yahK putative deaminase with metallo‑dependent hydrolase domain/aldehyde reductase, NADPH‑dependent
* ? NC_000913 566777 =NA (NA)2112 (44.970) 251/262 NT 97.7% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 = 572471 49 (1.040)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 = 56684130 (0.630)9 (0.260)
+34 bp
8/196 NT 23.5% noncoding (65/1258 nt) IS3 repeat region
?NC_000913 = 572471 49 (1.040)coding (6/456 nt) ybcN DLP12 prophage; DNA base‑flipping protein
* ? NC_000913 958761 =42 (0.890)3 (0.070) 3/248 NT 7.6% intergenic (+20/‑51) serC/aroA phosphoserine/phosphohydroxythreonine aminotransferase/3‑phosphoshikimate 1‑carboxyvinyltransferase
?NC_000913 958782 = 34 (0.770)intergenic (+41/‑30) serC/aroA phosphoserine/phosphohydroxythreonine aminotransferase/3‑phosphoshikimate 1‑carboxyvinyltransferase
* ? NC_000913 1061939 =41 (0.870)3 (0.070) 3/248 NT 7.2% coding (141/600 nt) torD trimethylamine‑N‑oxide reductase‑specific chaperone
?NC_000913 1061960 = 39 (0.880)coding (162/600 nt) torD trimethylamine‑N‑oxide reductase‑specific chaperone
* ? NC_000913 1239530 =35 (0.740)4 (0.090) 3/252 NT 10.8% coding (652/1071 nt) dadX alanine racemase 2
?NC_000913 1239563 = 33 (0.730)coding (685/1071 nt) dadX alanine racemase 2
* ? NC_000913 1456809 =41 (0.870)3 (0.060) 3/260 NT 7.2% coding (380/1071 nt) paaE phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
?NC_000913 1456830 = 37 (0.790)coding (401/1071 nt) paaE phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
* ? NC_000913 = 146052139 (0.820)4 (0.090) 3/250 NT 8.9% coding (51/423 nt) paaI phenylacetyl‑CoA thioesterase
?NC_000913 = 1460536 45 (1.000)coding (66/423 nt) paaI phenylacetyl‑CoA thioesterase
* ? NC_000913 = 162176347 (0.990)4 (0.090) 4/258 NT 7.8% coding (432/1188 nt) ydeE dipeptide exporter
?NC_000913 = 1621777 48 (1.040)coding (446/1188 nt) ydeE dipeptide exporter
* ? NC_000913 = 174484855 (1.160)4 (0.090) 4/244 NT 7.1% intergenic (+18/+23) mdtK/ydhQ multidrug efflux pump MdtK/adhesin‑like autotransporter YdhQ
?NC_000913 = 1744854 54 (1.240)intergenic (+24/+17) mdtK/ydhQ multidrug efflux pump MdtK/adhesin‑like autotransporter YdhQ
* ? NC_000913 = 204573255 (1.160)3 (0.070) 3/250 NT 5.3% coding (795/7077 nt) yeeJ inverse autotransporter adhesin
?NC_000913 = 2045738 55 (1.230)coding (801/7077 nt) yeeJ inverse autotransporter adhesin
* ? NC_000913 = 204890444 (0.930)5 (0.110) 5/250 NT 11.5% coding (3967/7077 nt) yeeJ inverse autotransporter adhesin
?NC_000913 = 2048914 35 (0.780)coding (3977/7077 nt) yeeJ inverse autotransporter adhesin
* ? NC_000913 = 247246955 (1.160)3 (0.060) 3/258 NT 5.2% coding (413/495 nt) yfdN CPS‑53 (KpLE1) prophage; protein YzyA
?NC_000913 = 2472503 55 (1.190)coding (379/495 nt) yfdN CPS‑53 (KpLE1) prophage; protein YzyA
* ? NC_000913 2529006 =46 (0.970)3 (0.070) 3/252 NT 6.4% coding (1171/2016 nt) ligA DNA ligase
?NC_000913 2529035 = 44 (0.970)coding (1142/2016 nt) ligA DNA ligase
* ? NC_000913 = 303632946 (0.970)5 (0.110) 5/250 NT 10.3% intergenic (‑47/+44) prfB/recJ peptide chain release factor RF2/ssDNA‑specific exonuclease RecJ
?NC_000913 = 3036346 44 (0.980)intergenic (‑64/+27) prfB/recJ peptide chain release factor RF2/ssDNA‑specific exonuclease RecJ
* ? NC_000913 = 3114511NA (NA)3 (0.070) 3/250 NT NA intergenic (‑159/+39) pppA/yghJ prepilin peptidase/putative lipoprotein YghJ
?NC_000913 = 3114541 NA (NA)noncoding (29/34 nt) other REP219c
* ? NC_000913 = 316167253 (1.120)3 (0.060) 3/258 NT 5.3% coding (998/1413 nt) ftsP cell division protein required during stress conditions
?NC_000913 = 3161700 55 (1.190)coding (970/1413 nt) ftsP cell division protein required during stress conditions
* ? NC_000913 = 334184829 (0.610)4 (0.090) 4/252 NT 12.4% coding (583/987 nt) kdsD D‑arabinose 5‑phosphate isomerase KdsD
?NC_000913 = 3341856 29 (0.640)coding (591/987 nt) kdsD D‑arabinose 5‑phosphate isomerase KdsD
* ? NC_000913 = 3639355NA (NA)2 (0.050)
+CGAGACTGATTACA
3/236 NT NA intergenic (+214/+30) pitA/uspB metal phosphate:H(+) symporter PitA/putative universal stress (ethanol tolerance) protein B
?NC_000913 = 4137897 NA (NA)intergenic (‑240/+35) yijF/gldA conserved protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
* ? NC_000913 3810274 =NA (NA)3 (0.070) 3/250 NT NA coding (450/480 nt) coaD pantetheine‑phosphate adenylyltransferase
?NC_000913 3810291 = NA (NA)coding (467/480 nt) coaD pantetheine‑phosphate adenylyltransferase
* ? NC_000913 = 3944978NA (NA)4 (0.090) 3/252 NT 15.4% noncoding (1275/2904 nt) rrlC 23S ribosomal RNA
?NC_000913 = 4038811 23 (0.490)noncoding (1293/2905 nt) rrlA 23S ribosomal RNA
* ? NC_000913 = 397743047 (0.990)3 (0.070) 3/250 NT 6.3% coding (1153/1251 nt) wzxE lipid III flippase
?NC_000913 = 3977447 45 (1.000)coding (1170/1251 nt) wzxE lipid III flippase
* ? NC_000913 = 416834118 (0.380)3 (0.070) 3/244 NT 14.8% intergenic (+141/‑31) rrsB/gltT 16S ribosomal RNA/tRNA‑Glu
?NC_000913 = 4168363 18 (0.410)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA/tRNA‑Glu
* ? NC_000913 = 428921255 (1.160)5 (0.110) 5/250 NT 8.5% intergenic (+12/‑33) nrfA/nrfB cytochrome c552 nitrite reductase/periplasmic nitrite reductase penta‑heme c‑type cytochrome
?NC_000913 = 4289220 55 (1.230)intergenic (+20/‑25) nrfA/nrfB cytochrome c552 nitrite reductase/periplasmic nitrite reductase penta‑heme c‑type cytochrome