Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F107 I0 R1
|
44 |
28.8 |
1140136 |
90.5% |
1031823 |
142.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
100% |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 100.0%
| 58.8
/ ‑6.7
| 22 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/10); minor base C (1/0); total (12/10) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACG > NC_000913/760359‑760611
|
aaGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGGCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCt > 2:178159/1‑143 (MQ=255)
tGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTAc < 1:341584/143‑1 (MQ=255)
gAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGGCGGTGAGCGACTGGTCGCCTGGAGCGGTGCGGAAAACCTCGCTTACGCCAc > 1:128431/1‑143 (MQ=255)
cTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGACTTATGGCGATCGCCAGGCGATGGCTGCCGGGGAGAAACTGGTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCCCGCt > 2:411542/1‑143 (MQ=255)
aaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTCCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgat < 2:363883/143‑1 (MQ=255)
cACGGGGCCGGAAGCAGTGGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTc < 2:129748/143‑1 (MQ=255)
cGGAAGCAGTTGAAACGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc < 2:463154/143‑1 (MQ=255)
cAGTTGGAATGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCg < 2:415494/143‑1 (MQ=255)
gTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg > 1:140025/1‑143 (MQ=255)
aTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGa < 1:568490/143‑1 (MQ=255)
aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCAGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTAGCGGTACctt > 1:382931/1‑143 (MQ=255)
aTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGGATTCCGGTGCGCCTGTCGGGTGAAGACTCCGGCGGCGGGTCcgtctt > 1:9011/1‑143 (MQ=255)
ttATGGCGATCGCCAGGCAATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCGTACGACACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGCAGCCTCCGGTCGCGGTACcttctgcg > 1:133028/1‑140 (MQ=255)
aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 2:410357/143‑1 (MQ=255)
tGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAcc > 1:176645/1‑143 (MQ=255)
ggggacggcggcCGGTGAGAAACGGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa < 1:437532/137‑1 (MQ=255)
ggCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTGCCTTCTTCCACCGCCACGCGGTGATCcaca > 1:253675/1‑143 (MQ=255)
gcgggTGAGCAGCTGTGCGACTCGAGCGGTGCGGAAAACCTCGCTTCCGCCACGCTGGTTGATGAAGGCCTCCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa < 2:287600/140‑1 (MQ=255)
ggTGAGCAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCGAccaccaacc > 2:534043/1‑140 (MQ=255)
ggTGAGAAACTGTTCGACTGGAGCGGTGCGGGAAACCTCGCGTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGAGGTACCTTCTTACACCACCACGCGGTGATCCACAACCAggc > 1:215949/1‑141 (MQ=255)
agaAACGGGTCGACTGTAGCGGTGCGGAAAACCGCGCGTACGCCACGCTGGTCGATGAAGGCATTCCGGTTCGCCTGTCGGTTGAACACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAAc < 1:436107/143‑1 (MQ=255)
gaAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATGCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACg > 2:441390/1‑143 (MQ=255)
|
AAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACG > NC_000913/760359‑760611
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
