Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F71 I0 R1	83	59.3	2271602	90.4%	2053528	142.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	760,485	G→A	100%	G594S (GGC→AGC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	760,485	0	G	A	96.2%	110.7 / ‑5.2	44	G594S (GGC→AGC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (20/21); minor base C (2/0); total (22/22)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTAC  >  NC_000913/760351‑760622

ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCACAACATGTCACTC‑AAGGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCg                                                                                                                                   >  1:519335/1‑143 (MQ=255)

          ggcgcTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCTGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg                                                                                                                         <  2:383447/139‑1 (MQ=255)

                gccggAGCTGGCGACACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg                                                                                                                   <  1:287031/140‑1 (MQ=255)

                 cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑GAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGGGGTGCGGAAAACGTCGCTTACGc                                                                                                                  >  1:367588/1‑143 (MQ=255)

                  aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGGTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAGACTGTTCGACTGGAGCGGTGCGGGAAACCTCGCTTACGcc                                                                                                                 >  1:942258/1‑143 (MQ=255)

                  aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAACACCCCGCTTACGcc                                                                                                                 >  1:142376/1‑143 (MQ=255)

                        tGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTg                                                                                                           >  1:238664/1‑143 (MQ=255)

                             aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTAGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgg                                                                                                      >  1:38508/1‑142 (MQ=255)

                               aCGCATCAGCACGGTGCCGGAAGCCGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg                                                                                                    <  2:922109/143‑1 (MQ=255)

                               aCGCATCAGCACGGTGCCGGAAGCATTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg                                                                                                    <  1:172996/143‑1 (MQ=255)

                               aCGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatg                                                                                                    <  2:463257/143‑1 (MQ=255)

                                cGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGAGCTGGCTGACGCCACGCTGGCtgatga                                                                                                   >  1:998025/1‑143 (MQ=255)

                                 gCATCAGCACGGTGCCGGAAGCAGTTGATATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa                                                                                                  <  2:392686/143‑1 (MQ=255)

                                 gCATCAGCACGGTGCCGGAAGCAGTGGAAATGCAGGCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa                                                                                                  <  1:27694/143‑1 (MQ=255)

                                  tagcAGCACGGTGCGGGAAGCAGTTGAAATGCACTCTCGCGCTGC‑CAAGATTTATGGCGGTCGCCGGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAg                                                                                                 <  2:881013/140‑1 (MQ=255)

                                  cATGAGCACGGTGCCGTAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg                                                                                                 <  2:472171/143‑1 (MQ=255)

                                            gTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCAATGGCTGCCGGTGAGAAACTGTTCGAATGCAGCGGTGCGGAAAACCTCGGTTACGCGACGCTGGATGCTGAGAGCGTTGCGGt                                                                                       >  2:311407/1‑143 (MQ=255)

                                                cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                                   <  2:624259/143‑1 (MQ=255)

                                                cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                                   >  1:265387/1‑143 (MQ=255)

                                                cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                                   <  1:961311/143‑1 (MQ=255)

                                                cGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                                   >  1:166026/1‑143 (MQ=255)

                                                     gCAGTTGAAATGCAGACTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGGTCGACTGGAGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTTGCTCCAGGCATTCCGGTTCGCCTGTc                                                                              >  2:953789/1‑143 (MQ=255)

                                                       ggTTGAAAGGCAGTCGCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg                                                                            <  2:1071146/142‑1 (MQ=255)

                                                       aGTGGAACTGCAGCCGCGCCTTGC‑CAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg                                                                            <  2:1037791/143‑1 (MQ=255)

                                                        ggtGAAATGCAGTCTCGCGTTGC‑CAAGATTTGTGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTTTCggg                                                                           <  2:939150/141‑1 (MQ=255)

                                                        gTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGTGATCGCCAGGCGATGGCTGCCGGTGAGAGACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCggg                                                                           >  2:1113832/1‑143 (MQ=255)

                                                                 cAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCACTCCGGCTCGCCTGTCGGGTGCGGACTc                                                                  >  2:55324/1‑143 (MQ=255)

                                                                  aGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGAGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTcc                                                                 >  1:732221/1‑143 (MQ=255)

                                                                    tctcGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTCATGACGCCCCTCCGGTTCCCCGCTCGGGGGAACCCTcccg                                                               >  2:1114458/1‑141 (MQ=255)

                                                                    tctcGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGCTGAAGGCATCCCGGCTCGCCTGTCGGGCGAACACTcccg                                                               >  2:120575/1‑141 (MQ=255)

                                                                        gcgTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGAGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgc                                                           >  1:423500/1‑143 (MQ=255)

                                                                         cgTTGC‑CAAGATTCATGGCGAGCGCCAGGCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgcg                                                          <  1:345914/143‑1 (MQ=255)

                                                                           ttGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTCCGGCTTCCCTCCCGTATGGGCGGAATGAACAAGAAGGCATTCCGGTTCGCCCGTCGGGTGCAGACcgcggtcgcggt                                                        >  2:1103167/1‑143 (MQ=255)

                                                                                     tttATTGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc                                               <  2:829921/143‑1 (MQ=255)

                                                                                        aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGCGAAGACTCCGGTCGCGGTACCTTCTTCCAc                                            <  1:784213/143‑1 (MQ=255)

                                                                                                 gCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGAGGGGTTGCGCCCTCACGCCGCGCATGGCGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg                                   <  2:238664/143‑1 (MQ=255)

                                                                                                     ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa                               <  1:1029165/143‑1 (MQ=255)

                                                                                                                         aaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACACGGTGATCCACAACCAGTCTAACGGt           >  2:371622/1‑143 (MQ=255)

                                                                                                                              ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGGAGGCATTCCGGTTCGCCTGTCGGGTGAGGACTCCGGTCGCGGTGCCGTCTTCCACCGCGACGCGGTGGTCCACAACCAGTCTAACCGGTccgc      >  2:101917/1‑141 (MQ=255)

                                                                                                                              ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCAc      >  2:24314/1‑143 (MQ=255)

                                                                                                                              ttCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTACGGTCGCGGTACCTTCTTCCACCGCCACCCGGTGAACCACAGCCGATCTAAACGTCCCAc      >  2:925086/1‑143 (MQ=255)

                                                                                                                               tCGACGGGGGCGGGGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGGAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACTCGGTGATCCACAACCAGTCTAACGGTTCCACt     <  2:589924/143‑1 (MQ=255)

                                                                                                                                 gACGGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCCCGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa   <  1:311111/143‑1 (MQ=255)

                                                                                                                                  aCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTac  <  1:483774/143‑1 (MQ=255)

TTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGC‑CAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTAC  >  NC_000913/760351‑760622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A