Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A21 F59 I1 R1
|
23 |
54.9 |
2144786 |
90.8% |
1947465 |
141.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 91.9%
| 93.3
/ NA
| 37 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (13/21); total (15/22) |
| Fisher's exact test for biased strand distribution p-value = 5.54e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760361‑760621
|
gcggcggCAGGAGCTGGCGAGACGCATCAGGACGGTGCCGGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCg < 2:485594/137‑1 (MQ=255)
gcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCt < 1:71491/143‑1 (MQ=255)
aGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACg > 1:896101/1‑143 (MQ=255)
cTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCt < 1:669538/143‑1 (MQ=255)
gCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCGAGATGGATGGCGATCGCCAGGCGAGGGCTGCCGGAGAGAGACGGCTCGACTGGGGGGGCGCGGAAGAACCCGCTTACGCCACGCTggg > 2:982137/1‑142 (MQ=255)
cGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGtt < 2:492254/143‑1 (MQ=255)
gAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTCCGCCACGCTGGTtg > 1:189358/1‑143 (MQ=255)
aaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtggt > 1:927206/1‑141 (MQ=255)
gCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGaa < 1:171627/143‑1 (MQ=255)
gCATCAGCACGGTGCCGGAAACAGTTGAAATGCAGTCTCGCGTTGCCGGGAATTATGGCGGTCGGGAGGCGATGGCTGCCGGTGAGAAACTGTTGGACTGGGGCGGTGCGGAAAATATCGATTACGCCACGCTGGTTGATGaa > 2:572141/1‑143 (MQ=255)
aTCAGCACGGTGCCGGAAGCAGTGGAAATGCAGTCTCGCGTTGCCAAGATTTAGGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAgg < 1:973941/143‑1 (MQ=255)
tCAGCACGGTGCCGAAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGAACGCCAGGCGATGGCTGCCCGAAAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCACGCTTACGCCACGCAGGTTGATAAAGGc > 2:339299/1‑143 (MQ=255)
gTGCCCGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATCTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGTCACGCTGGTTGATGAAGGCATTCCGGt < 1:856231/143‑1 (MQ=255)
gCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGAGAGGCGATGGCTGCCGGTGAGAAACTGTTAGACTGGAGCGGTGCGGAAAACGTCGCTTACGCCAGGCTGGTTGGTGAAGGCATTCCGGttg > 2:543094/1‑142 (MQ=255)
ccGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCg > 1:911914/1‑143 (MQ=255)
cAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCg < 1:756107/143‑1 (MQ=255)
agctGCGGTCTCGCGTGTCGAAGAGTGATGGCGTTCGCCTGGCGATGGCTGCCGGTGAGAAGCTGTTCGACTGGAGCCGTGCGTAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:159527/140‑1 (MQ=255)
aaaTGCAGTCTCGCGTGGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAATCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGaa < 2:376580/143‑1 (MQ=255)
ccgccgcgcgTTGCCAAGAGTGATGGCGATCGCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTc < 2:768222/137‑1 (MQ=255)
gttgtctcgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTcc < 1:1067965/136‑1 (MQ=255)
gTTGCCAAGATTGATGGCGCTCGCCAGGCGATGGCTGCCGGTGAACGATGGGGCGGCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCgg < 1:1012955/143‑1 (MQ=255)
aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCTGAAAACCTCGTTTACGGTACGCTGGTTGATGAAGGCATTCCTGTTCGCCCGTCGGGTGAAGACTCCGGTCGCGGTACctt > 1:780380/1‑143 (MQ=255)
aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCAGTCCGGATCGCCTCACGCGTGAAAACTCTGGTggcgatgactc > 2:711132/1‑136 (MQ=255)
ttgaTGGCGATCGCGAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc < 2:1047104/140‑1 (MQ=255)
gggCGGTCGGGAGGCGATGGTTGCTCGTTAGAGCTGGTTCGGCTGGGGCGGTGCGGAATGCCCCGCTTACGCCACGCTTGTTGATGAAGGCATTCCGGTTCGCCGGTCGGGTGAAGTCTTCGGTCGCTGTACCTGCTTCCAcc < 2:654244/142‑1 (MQ=255)
ggtggtCGCGAGGCGATGGCTGCTGGTGAGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTGCGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCg < 2:1067938/138‑1 (MQ=255)
cGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTGCTTCCACCGcc > 1:447690/1‑143 (MQ=255)
ccAGGCGATGGCTGCCGGTGAGAAACTGTTCGCCTGGAGCGAAGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCCTCTTCCACCGCCAcgcgc > 2:873088/1‑142 (MQ=255)
cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGt > 1:603282/1‑143 (MQ=255)
ggCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGa < 1:1049104/143‑1 (MQ=255)
gATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCCGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATcc < 1:688634/143‑1 (MQ=255)
tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGGTGACGGCATTCCGCCGCGCCGGGCGGGTGAAGACTCCGCTCGCGGCACCTTCCTCCACCGCCCCCCGATCATCCACAAcc > 2:123578/1‑143 (MQ=255)
gCCGGTGTTAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa < 2:984076/143‑1 (MQ=255)
aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg < 2:269174/79‑1 (MQ=255)
aaaCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg > 1:269174/1‑79 (MQ=255)
gTTCGACTGGAGCGGTGCGGAGAAGCCCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCa < 1:912328/143‑1 (MQ=255)
gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTa < 1:42910/143‑1 (MQ=255)
|
GCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA > NC_000913/760361‑760621
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
