Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F111 I1 R1
|
23 |
51.1 |
2003682 |
90.7% |
1817339 |
142.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 94.1%
| 77.5
/ NA
| 35 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (19/13); total (21/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC > NC_000913/760345‑760624
|
aCAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGCTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGCGAGAAAATGTTCGACTGgcgc > 2:280772/1‑143 (MQ=255)
gTTGAGAGGGGGCGCCGGCAGGGGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGc < 2:801591/143‑1 (MQ=255)
ttGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGGGCg > 1:850158/1‑143 (MQ=255)
agaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGCTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaa > 1:544313/1‑143 (MQ=255)
agaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaa > 2:955658/1‑143 (MQ=255)
gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAGGGAGTTGAAATGCAGTGTCGCGTTGCCAAGATTTATGGCGATGGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGGGCGGTGCGGAGAGCCTCg > 2:213894/1‑143 (MQ=255)
cgcCTGCATGAGCTGGCGAAACGCACCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGc < 2:216405/143‑1 (MQ=255)
cccgCAGGACCTGTCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCtt < 1:290653/140‑1 (MQ=255)
tGCAGGCGCTGGCGAGACGCATCAGGACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTAc < 1:552594/143‑1 (MQ=255)
gCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGACACTGTGCGGCAGCCCTCGCTTagg > 2:744323/1‑141 (MQ=255)
gCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGc > 1:292043/1‑143 (MQ=255)
gCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGCTGCGGACAACCTCGCTTACGTCAGGCTGGt > 2:908743/1‑143 (MQ=255)
cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGCAAACCTCGCTTGCGCCACGCTGGTTGGTGAAGGCCTTc > 1:24279/1‑143 (MQ=255)
cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGACAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGCAGGCATTc > 1:165982/1‑143 (MQ=255)
tGCCGGAAGCAGTGGAGATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGtt < 2:275250/143‑1 (MQ=255)
gTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGGTCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAGAACCTCGCTTACGCCACGCGGGTTGATGAAGGCATTCCGGTTCGCGCGTCgag > 2:587535/1‑141 (MQ=255)
tggaaTGCAGTCTCGCGTTGCCAAGATTGATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTg < 2:629117/140‑1 (MQ=255)
ggaaTGCAGCCGCGCGTTGCCAAGATGTATGGCGATCGCCGGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGa < 2:689495/141‑1 (MQ=255)
gAAATGCAGTCTCGCGTTGCCAAGCTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGCGCGGAAACCCTCAGGTACGCCACGAGGGTTGGTGAAGGCATTAAGGTTCGCCTGTCGGGGGa > 2:785718/1‑143 (MQ=255)
tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATTGCTGCCGGGGAGAAACTGTTCGACTCGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCATGTCGTGAGAAGAc > 2:71562/1‑143 (MQ=255)
cgcgTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTCCCGGTGAGGCACTGTTCGACTGCAGCGGTGCGGAACACCTCGCTTACGCCACGCTGGTTGATGGAGGCAGTCCGGCTCCGCTGTCGCGTGCACCCTCCGGTcg > 2:814109/1‑143 (MQ=255)
tGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa < 1:666346/143‑1 (MQ=255)
gagggtttAGGGCGCTCGCCAGGCGATGGCGGGCGCGGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCGACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACct < 2:379357/138‑1 (MQ=255)
aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 1:930619/143‑1 (MQ=255)
aTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAc < 2:415488/143‑1 (MQ=255)
cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTGACGCCACGCTGGCTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGt > 1:573264/1‑143 (MQ=255)
aGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTg > 1:110498/1‑143 (MQ=255)
gATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATcc > 1:825635/1‑143 (MQ=255)
tGGCTGCCGGTGAGAAGCTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTGACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCcac < 2:633365/143‑1 (MQ=255)
tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTACGCCTGACGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCCCGCGGTGATCCACAAcc > 2:373370/1‑143 (MQ=255)
tGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTGCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTa > 1:345546/1‑143 (MQ=255)
gagaAACTGGTCGACTGGAGCGGTGCGGAAAGCCTCGCTTACGCCACGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTaa < 2:178696/143‑1 (MQ=255)
aCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCCGCCGGGTGAAGACTCCGGTCGCGGTACCTTCGTCCACCGGCACGCGCGGAGCCACAACCAGGCGAACgcct > 2:24444/1‑140 (MQ=255)
gACTGGAGCGTTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGGAGGCGTTCCGGTTCGCCTGTCGGCTGAAGACTCCGGACGCGGTCGCGTCTTCCACCGCCACGCGGCGATCCCCACCCAGCCGAACCGTTCCAcgcc > 2:494212/1‑140 (MQ=255)
gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGCAGACTCCGGTCGCGGTGCCTTCTCCCACCGCCTAGCGGTGTTCCACGACCAGTCTAGCGGTTGCACTTa > 1:197792/1‑143 (MQ=255)
tGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTacac < 2:528568/143‑1 (MQ=255)
|
ACAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTACAC > NC_000913/760345‑760624
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
