Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F66 I1 R1
|
21 |
40.5 |
1611144 |
86.0% |
1385583 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
760,485 |
G→A |
G594S (GGC→AGC) |
sucA → |
subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 760,485 | 0 | G | A | 96.5%
| 64.5
/ NA
| 29 | G594S (GGC→AGC) | sucA | subunit of E1(0) component of 2‑oxoglutarate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/14); minor base C (1/0); total (15/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACT > NC_000913/760354‑760619
|
agaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGCTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaa > 1:396235/1‑143 (MQ=255)
gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATAGCGATCGCCAGGCGATGACTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa > 1:10937/1‑143 (MQ=255)
gAAGCGACTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAcc < 1:384677/143‑1 (MQ=255)
ggcgcTTGCAGTGGCGGGCGACACGCAGCGGTGCGGGGCCGGAAGCAGTTGAAATGCAGTGTCGTGTTGCCAAGCATTATGGCTATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTc < 2:195344/142‑1 (MQ=255)
gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGCGAGAAACTGTTCGACTGCAGCGGTGCGGCAAAccccc > 2:319175/1‑140 (MQ=255)
aGCTGGCGAAACGCATCAGCAAGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCGGGCGAGAAACTGAACGACTGGAGCGGTGAGCGAAACCTCGCATACGCCagc > 2:320685/1‑141 (MQ=255)
ggCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACACTgg > 1:721342/1‑143 (MQ=255)
cAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCa > 1:789208/1‑143 (MQ=255)
gCACGGTGCCGGAATCAGTTGAAATGCAGTCTCGCTTTGCCAAGTTTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCAtt < 1:256437/143‑1 (MQ=255)
agTGCCGGAAGCAGTTGAAGTGCAGTCTGTCGTTGCCAGGTTTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAGGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCgg < 1:796158/142‑1 (MQ=255)
gTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGt > 2:388499/1‑143 (MQ=255)
ggAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCGCGCCTACGCCACGCTGGTTGAGGAAGGCATTCCGGTCCGcc > 2:563562/1‑143 (MQ=255)
aGTTCAAGTGCAGTCTCGCGTAGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCgg < 2:133287/143‑1 (MQ=255)
aaaTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCAACAACCGATGGCAGCAGATGAGAAACTGTTCGACTGAAGCGATGCGGAAAAACAGGCTAACGCCACACTGGATAATGAAGGCATGCAGGTTCGGCTGGCggtttaa > 2:54391/1‑138 (MQ=255)
tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTACGGAAAACATCGCTTACGCAACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGAc > 1:648135/1‑143 (MQ=255)
gcgTTGCCACGATTTATGGCGATCGCGAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTcgc < 2:583010/143‑1 (MQ=255)
tGCCAAGCTTCCTGGTAATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa < 1:490845/143‑1 (MQ=255)
tGCCAAGATTTATTGCTATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTa < 1:215704/143‑1 (MQ=255)
aaGATTTATGGCGATCGCCAAGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGATTCGCCAGTCGGGTGAAGACTCCGGTCGCGGTACctt > 2:501853/1‑143 (MQ=255)
aGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCAGTCGCGGTACcttc > 1:645888/1‑143 (MQ=255)
gATTTATGGCTATCGCGAGGCGATGGCTGGCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACcttct < 2:105649/143‑1 (MQ=255)
atcccgaGGCGATGGATGCCGGTGAGACCGTGTTCGTCTGGAGCGCTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAc < 2:460790/137‑1 (MQ=255)
aTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAc < 2:434550/143‑1 (MQ=255)
cGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGAGGAAGGCATTCCGGTTCGCGTGTGGGGGGGAGACGCCGGTCGCGGTCCCTCCTTCCACCGCCgcgc > 1:326179/1‑143 (MQ=255)
aGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTg > 1:779560/1‑143 (MQ=255)
ctgttgCTGTCGGTGATAAACTGTTCGACTGGAGAGGTGCGGAAACCCTCGCTTACGCCACGCTGGTTGCTGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc < 1:498274/138‑1 (MQ=255)
ccGGTGAGAAAGTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAg < 1:600943/143‑1 (MQ=255)
aCTGTTCGACTGGAGCGGGGGGGAAGTGCGCGGTTACGGCTCGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGACGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGtt < 2:147775/143‑1 (MQ=255)
tCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGGGGTGCTCCACAGCCCGAATGACAGTTCCACt > 2:731460/1‑143 (MQ=255)
|
AGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACT > NC_000913/760354‑760619
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
