Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F21 I1 R1 19 60.0 2284426 90.7% 2071974 142.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 760,485 G→A G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913760,4850GA97.8% 121.9 / NA 47G594S (GGC→AGC) sucAsubunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (19/26);  minor base C (0/1);  total (19/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA  >  NC_000913/760346‑760621
                                                                                                                                           |                                                                                                                                         
cAAGGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCg                                                                                                                                        <  1:262258/143‑1 (MQ=255)
cAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCg                                                                                                                                        >  2:757970/1‑143 (MQ=255)
      tGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGGAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCgg                                                                                                                                  <  1:279697/143‑1 (MQ=255)
         gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaac                                                                                                                               >  1:633729/1‑142 (MQ=255)
         gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa                                                                                                                               <  1:934491/143‑1 (MQ=255)
         gaTGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGaaa                                                                                                                               >  1:579351/1‑143 (MQ=255)
                     gCAGGGGCTGGCGAATCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCTGTCTCGCGTTGCCACGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg                                                                                                                   <  2:404358/143‑1 (MQ=255)
                     gCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGTCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACg                                                                                                                   <  1:571273/143‑1 (MQ=255)
                               gCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACACCCTCGCTCCCGCCACGCTGGt                                                                                                         >  2:250289/1‑143 (MQ=255)
                                 gAAACGCAACAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtg                                                                                                       <  2:119804/143‑1 (MQ=255)
                                  aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga                                                                                                      <  1:1134186/143‑1 (MQ=255)
                                     cGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgatga                                                                                                   <  1:972977/143‑1 (MQ=255)
                                       cATCAGCACGGTGCCGGAAGCAGTTGAAATGAAGTCTCGCGTTGCCAAGATTTATGGCGATCGCGAGGCGATGGATGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGGAAACCTAGCTTACGTCAAGCTGGTTGATGAAg                                                                                                 >  1:501221/1‑143 (MQ=255)
                                         tCAGCACGGTTCCGGAAGCAGTTGAAATGCAGTCTCGCATTGCCAGCATTTATGGCGATAGCCAGGCGATGCCTGCCGCTGAGAAACTGTTCGACTGGAGCGGTGCGGAACAACTCGCTTACGCTACGATGGTagctgagcgc                                                                                               >  2:1028133/1‑139 (MQ=255)
                                             cACGGTGCCGGAAGCAGTTGCAATGCAGTCTCACGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGCTGATGAAGGCATTc                                                                                           >  2:595740/1‑143 (MQ=255)
                                                  cgCCCGAAGCAGTTGAAATGCAGGCTCGCGGTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAACCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGt‑t                                                                                     <  1:229636/142‑1 (MQ=255)
                                                    ccGGAAGCAGTTGAAAGGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCg                                                                                   <  2:1127264/143‑1 (MQ=255)
                                                     cGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGGAACTGTTGGGCTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGC‑TCGc                                                                                  >  2:43807/1‑143 (MQ=255)
                                                     cGGAAGCAGGTGAAATGCAGGCGCGCGTTGCCAAGAGTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGc                                                                                  <  1:387940/143‑1 (MQ=255)
                                                            aGTTGAAATGCAGTCTCGCGTTGCCAAGATCTATGGCGTGCGTGAGGTGGTGTCGGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCgg                                                                           >  1:1083920/1‑143 (MQ=255)
                                                              ttGAAATGCAGTCGCGGGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCGGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGt                                                                         <  2:57367/143‑1 (MQ=255)
                                                              ttGAAATGCAGTCGCGCGGTGCCAAGATTTATGGCGAGCGCCAGGCGATGGCTGCCGGTGAGAAGCGGTTCGACGGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGt                                                                         <  2:925090/143‑1 (MQ=255)
                                                                 acgtGCGGTCTCGCCGTGCCAGGGTTTACGGCCATCGCCAGGCGGTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTACCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGaa                                                                      <  2:243523/140‑1 (MQ=255)
                                                                 aagtGCTGTCTCGCGTTGCCAATATTTATGGCGATCGCGAGGCGCTGGCTGCCGGTGAGAACCTGTGCGACTGGGGCGGTGCGGGAACCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGaa                                                                      <  2:30641/140‑1 (MQ=255)
                                                                         tctcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGGAAACCTCGCTTACGCCACGCTGGTTGATGGAGGCATTCCGGT‑TCGCCTGTCGGGTGAGGACTCCgg                                                              >  1:991628/1‑143 (MQ=255)
                                                                           tcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTc                                                            >  2:643886/1‑143 (MQ=255)
                                                                             gcgTTGCCAAGATTTATGACGATCGCCAGGCGATGGCTGCCGGTGAGACACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCATACGCCACGCTGGGTGATGGAGGCAGCCCGGT‑TCCCCTGCCGGGTGGAGACCCCgggagc                                                          >  2:613254/1‑139 (MQ=255)
                                                                               gTTGCCAAGATTTGTTGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCgg                                                        <  2:1000389/143‑1 (MQ=255)
                                                                                    cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGGAGACTCCGGGCGCGGTACct                                                   >  2:969730/1‑143 (MQ=255)
                                                                                      aGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGC‑TCGCCTGCCGGGTGAAGACTCCGGTCGCGGTACctgc                                                 >  2:518111/1‑141 (MQ=255)
                                                                                         tttATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTTCGGAAACCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc                                              <  2:582087/143‑1 (MQ=255)
                                                                                         tttATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCA‑TCAGTTAAAGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCttcttc                                              >  2:802478/1‑143 (MQ=255)
                                                                                             tGGCGATCGCCCGGGGATGGCTGCCGGTGAGATACTGTTCGACTGGAGCGGTGCGGCGCTTCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGTCTCCGTTCGCGGTACCTTCTTCCAcc                                          <  2:131665/143‑1 (MQ=255)
                                                                                             tGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCAcc                                          <  1:395392/143‑1 (MQ=255)
                                                                                                     gCCAGGCGATGGCTGGCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg                                  <  2:1124651/143‑1 (MQ=255)
                                                                                                     gCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAATCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGCCTGACGACGCCTGTCGTGCTACCTTCTTCCACCGCCAcgcg                                  >  2:500216/1‑143 (MQ=255)
                                                                                                     gCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCAcgcg                                  <  2:1023267/143‑1 (MQ=255)
                                                                                                          gCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGAt                             <  1:757970/143‑1 (MQ=255)
                                                                                                              tGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCcac                         >  1:1106087/1‑143 (MQ=255)
                                                                                                               ggCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCCGTGATCcaca                        <  2:579351/143‑1 (MQ=255)
                                                                                                                 cTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc                      <  1:643886/143‑1 (MQ=255)
                                                                                                                 cTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAAc                      <  1:629301/143‑1 (MQ=255)
                                                                                                                     cGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCCTTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCGTCTTCCACCGCCCCGAGGTGATCCACAACCAGt                  >  1:462138/1‑143 (MQ=255)
                                                                                                                        tGATAAACTGTTCGACTTGCGCGGTGTGGAAATCCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCTGT‑TCGCCTGTCGGCTGATGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTa               <  2:306997/143‑1 (MQ=255)
                                                                                                                                 gtgcGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCa      <  1:161514/140‑1 (MQ=255)
                                                                                                                                   tCGACGGGAGCCGTGCCGAAATCCTCGCTCACGCCACGCTGGTGGATGACGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACt    <  2:471555/143‑1 (MQ=255)
                                                                                                                                     gACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCCCAACCAGTCTAACGGTCCCACtcc  >  2:329677/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CAAAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGT‑TCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCCACTTA  >  NC_000913/760346‑760621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A