Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F59 I0 R1 60 41.1 1578944 90.5% 1428944 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 760,485 G→A 100% G594S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913760,4850GA100.0% 101.0 / ‑6.7 43G594S (GGC→AGC) sucAsubunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/17);  minor base C (1/1);  total (25/18)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCC  >  NC_000913/760348‑760616
                                                                                                                                         |                                                                                                                                   
gaGTTGAGAGGAAGCGCCTGCAGGAGCGGGAGAAACGGATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGt                                                                                                                                <  2:468966/142‑1 (MQ=255)
          gAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAAcc                                                                                                                      >  1:361040/1‑143 (MQ=255)
             gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACcgcg                                                                                                                   >  1:393809/1‑143 (MQ=255)
             gcgcCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAGACCTCg                                                                                                                   >  1:688419/1‑143 (MQ=255)
                    cAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCCGGCGATGGCTGCCGGTGAGAAACTGTTAGACTGGAGCGGTGCGGAAGACCTCGCTTACGc                                                                                                            >  2:594501/1‑143 (MQ=255)
                     aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAATACCTCGCTTACGcc                                                                                                           >  1:153139/1‑143 (MQ=255)
                     aGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGAGCGGCAGGCGATGGCTGCCGGGGAGACACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGcc                                                                                                           >  1:545312/1‑143 (MQ=255)
                        aGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACg                                                                                                        <  2:618446/143‑1 (MQ=255)
                               gAAACGCATCAGCACGGTGCCCGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGCTtg                                                                                                 >  1:317337/1‑143 (MQ=255)
                                agaCGCAGCAGCACGGTGCCGGAAGCAGTTGAAATGCAGCCTCGAATCGCTGCACTTCATGGCGTTCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga                                                                                                <  1:573166/141‑1 (MQ=255)
                                aaaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtga                                                                                                <  1:770356/143‑1 (MQ=255)
                                 aaCGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGGCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTtgat                                                                                               <  1:636697/143‑1 (MQ=255)
                                     cATCAGGACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAg                                                                                           <  1:223056/143‑1 (MQ=255)
                                      aTCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCAAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGACACACTCACTTAAGCTGCGCTGATTGATGaaag                                                                                          >  2:3864/1‑141 (MQ=255)
                                          gCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAACACCTCGCTTACGCCTCGCTGGTTGATGAAGGCAtt                                                                                      >  1:347912/1‑143 (MQ=255)
                                          gCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGACGTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTAGGCCACGCTGGTTGATGAAGGCAtt                                                                                      >  1:498539/1‑143 (MQ=255)
                                           cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGCACACCTCGCTTACGCCACGCTGGCGGACGACGGCATTc                                                                                     >  2:400567/1‑143 (MQ=255)
                                           cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCCTTc                                                                                     >  1:524894/1‑143 (MQ=255)
                                           cACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGAGAGAAACTGTTCGACTGGAGCGGTGAGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCAtgc                                                                                     >  2:604804/1‑141 (MQ=255)
                                                   cgcaaGCCGTTTAAATGCAGTCTCGCGTTGCTCTGATTTCTGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGAGTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGc                                                                             <  2:211021/140‑1 (MQ=255)
                                                     gAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGATAAACTGTTCGACTGAAGCGGTGCGGAAAACCTCGCTTACGACACGCTGGTTGATGAAGGAATTCCGGTTCGCCt                                                                           >  2:535383/1‑143 (MQ=255)
                                                            ttGAACTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGt                                                                    <  2:653222/143‑1 (MQ=255)
                                                                 aTGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGa                                                               >  1:286021/1‑143 (MQ=255)
                                                                  tGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGACGGCATTCCCGTTCGCCCGTCGCGtcacgcc                                                              >  1:364407/1‑137 (MQ=255)
                                                                         tcGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTc                                                       >  1:392193/1‑143 (MQ=255)
                                                                                gCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGCGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAc                                                <  2:241264/143‑1 (MQ=255)
                                                                                 ccAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTAcc                                               >  1:381816/1‑143 (MQ=255)
                                                                                  cAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACct                                              >  1:121291/1‑143 (MQ=255)
                                                                                   aaGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGCAACTGTTCGACTGGAGCGGTGCGGACAACGTCGCTTACGCCCCGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGCCTCCGGGCGCGGTGCctt                                             >  2:93502/1‑143 (MQ=255)
                                                                                                     cAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGCGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGGCTCCGGGCGCGGTACCTTCTTCCACCGCCACGCGGt                           >  2:303229/1‑143 (MQ=255)
                                                                                                         cggtGGCTGCCTGTGAGAAACTGTTCGACGGGAGCGGTGCGGAAACCCTCGCTTACGCCGCGCTGGTTGATGGAGGCATTCCGGTTCGCCTGTCGGGTGCAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc                       <  2:180975/140‑1 (MQ=255)
                                                                                                         cgcgggCTGCCCGTGAGAAACTGTTTGACTGGCGCGGTGCGGACACCCTCGCTTACGCCACGCGGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc                       <  2:181237/139‑1 (MQ=255)
                                                                                                         cGATGGCTGCCGGTGAGAAACTGTGCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAGGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATc                       <  2:516234/143‑1 (MQ=255)
                                                                                                           aTGGCTGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCCCCCGGTGATccg                     >  1:16156/1‑142 (MQ=255)
                                                                                                                tGCCGGTGAGAAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTGCCTTCTTCCACCGCCACGCGGTGGTCCACAAcc                >  1:93253/1‑143 (MQ=255)
                                                                                                                 cccGGTGCGAAGCTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCa               <  1:594501/142‑1 (MQ=255)
                                                                                                                  ccGGTGAGAAACTGATCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACCCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACCACCAg              >  2:285952/1‑143 (MQ=255)
                                                                                                                   cGGTGAGAGACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTGACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGt             <  2:6036/143‑1 (MQ=255)
                                                                                                                    gtcgagaAGCTGTTCGGCTGGATCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTc            <  2:361040/140‑1 (MQ=255)
                                                                                                                        agaAACTGTACGACCGGAGCGGAGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAAc        <  1:749722/143‑1 (MQ=255)
                                                                                                                         gaAACTGTTCGACTGGAGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACg       >  1:449972/1‑143 (MQ=255)
                                                                                                                            atttttCGACTGTAGTTGTGCTGAAATTCTTTCTTACGCCACTCTGGTTGATGATGGCATTCCTGTTCGCCTGTCGGGTGAAGTCTCCGGTCGCGGTATCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGtt    <  2:619286/139‑1 (MQ=255)
                                                                                                                              tGTTCGACTGGAGCGGTGCGGCAGACCTCGCTTACGCCACGCTGGTTGATGAAGGCAGTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTcc  <  2:498539/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
AAGTTGAGATGAAGCGCCTGCAGGAGCTGGCGAAACGCATCAGCACGGTGCCGGAAGCAGTTGAAATGCAGTCTCGCGTTGCCAAGATTTATGGCGATCGCCAGGCGATGGCTGCCGGTGAGAAACTGTTCGACTGGGGCGGTGCGGAAAACCTCGCTTACGCCACGCTGGTTGATGAAGGCATTCCGGTTCGCCTGTCGGGTGAAGACTCCGGTCGCGGTACCTTCTTCCACCGCCACGCGGTGATCCACAACCAGTCTAACGGTTCC  >  NC_000913/760348‑760616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A